南方红豆杉组织培养提取物对α-糖苷酶活性的影响
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摘要
目的:研究南方红豆杉枝叶及其愈伤组织萃取紫杉醇后剩余部分对α-糖苷酶活力的影响。方法:将萃取紫杉醇后剩余部分分别加入α-淀粉酶、α-麦芽糖酶、α-葡萄糖苷酶不同微量反应体系中,分别用Bernfeld法、PNPG法、GOD法测定其酶活性,并采用Dixon作图法研究其动力学性质。结果:枝叶及其愈伤组织对α-麦芽糖酶和α-葡萄糖苷酶有明显的抑制作用,与拜唐苹的作用一致,其抑制存在量效关系,抑制α-麦芽糖酶活性[IC50=(218.49±18.24)mg/mL和(91.04±5.43)mg/mL]呈可逆性、竞争性抑制,抑制α-葡萄糖苷酶活性[IC50=(7.99±1.57)mg/mL和(19.87±2.52)mg/mL]呈不可逆性、竞争性抑制。结论:南方红豆杉枝叶及其愈伤组织萃取紫杉醇后剩余部分对α-糖苷酶均有一定的抑制活性,愈伤组织较优于枝叶。
Objective: To investigate the effects of the extracts from the branches and leaves of Taxus chinensis var. mairei and callus on alpha-glycosidase activity. Methods: The activity of different micro reaction systems like α-amylase, α-glucosidase and α-maltase, which were added with the extracts from the branches and leaves of Taxus chinensis var. mairei and callus, was determined by Bernfeld, PNPG and GOD methods. Kinetic parameters were determined by Dixon plot. Results: The aqueous extract, the branches and leaves of Taxus chinensis var. mairei and callus showed signii cant inhibition on α-maltase's and α-glucosidase's activity with dose-effect relationship, consistent with the role of acarbose, the inhibition from α-maltase [IC50=(218.49±18.24)mg/mL and(91.04±5.43)mg/mL] was reversible and competitive. The inhibition from α-glucosidase was [IC50=(7.99±1.57)mg/mL and(19.87±2.52)mg/mL] reversible and competitive. Conclusion: The extracts from the branches and leaves of Taxus chinensis var. mairei and callus all have activity against alpha-glycosidase, and callus is better.
Objective: To investigate the effects of the extracts from the branches and leaves of Taxus chinensis var. mairei and callus on alpha-glycosidase activity. Methods: The activity of different micro reaction systems like α-amylase, α-glucosidase and α-maltase, which were added with the extracts from the branches and leaves of Taxus chinensis var. mairei and callus, was determined by Bernfeld, PNPG and GOD methods. Kinetic parameters were determined by Dixon plot. Results: The aqueous extract, the branches and leaves of Taxus chinensis var. mairei and callus showed signii cant inhibition on α-maltase's and α-glucosidase's activity with dose-effect relationship, consistent with the role of acarbose, the inhibition from α-maltase [IC50=(218.49±18.24)mg/mL and(91.04±5.43)mg/mL] was reversible and competitive. The inhibition from α-glucosidase was [IC50=(7.99±1.57)mg/mL and(19.87±2.52)mg/mL] reversible and competitive. Conclusion: The extracts from the branches and leaves of Taxus chinensis var. mairei and callus all have activity against alpha-glycosidase, and callus is better.
引文
[1]中国科学院植物研究所.中国植物志.北京:科学出版社,2005:437-447
[2]梅兴国.红豆杉细胞培养生产紫杉醇.武汉:华中科技大学出版社,2003:4-5
[3]徐颖扉,张晶,舒琦瑾,等.南方红豆杉水提物诱导人肺腺癌A549细胞凋亡与紫杉醇抑瘤机制不同的研究.中华中医药杂志,2012,27(6):1696-1699
[4]阚常榕,崔炯谟,曹家庆,等.朝鲜产东北红豆杉提取紫杉醇后残液的化学成分研究.中草药,2010,41(11):1785-1787
[5]张丽,李彩芳,李晓梅,等.加拿大蓬α-葡萄糖苷酶抑制作用.河南大学学报:医学版,2008,27(4):39-41
[6]梁敬钰,吴斐华,鲍官虎,等.一种防治糖尿病的植物提取物及其制备方法与药物用途.中国:CN02144302.5.2004-04-14
[7]张学玉,曲玮,梁敬钰.红豆杉属植物化学成分及药理作用研究进展.海峡药学,2011,23(6):5-9
[8]李良松,冯仲科,刘德庆.红豆杉名实与功用通考.中国中药杂志,2011,36(12):1260-1263
[9]张翔宇,杜亚填,龚雪元.南方红豆杉愈伤组织生长动力学及紫杉醇代谢动力学研究.中草药,2012,43(5):990-994
[9]张芳芳,王鹏,姬丹丹,等.南方红豆杉愈伤组织培养条件的优化及紫杉醇积累的基因表达效应分析.中草药,2010,41(12):2058-2062
[11]熊耀康,余陈欢,张春椿,等.高效液相色谱指纹图谱法分析南方红豆杉药材的氯仿提取物析.色谱,2010,28(11):1067-1070
[12]吴慧平,陈建伟,许婷婷,等.骨碎补与夜交藤组合物对α-糖苷酶活性的影响.中国实验方剂学杂志,2012,18(5):181-184
[13]吴慧平,王洪兰,张喆,等.夏枯草迷迭香酸成分分析及其对α-糖苷酶活性影响研究.中药材,2011,34(11):1712-1715
[14]刘新颖,富晓楠,鲍玲,等.楮树皮中生物碱对α-糖苷酶和小肠糖吸收抑制作用研究.中华中医药学刊,2012,30(4):711-712
[15]刘自琴,黄惠华.绿茶和红茶浸提液对α-淀粉酶的抑制作用研究.现代食品科技,2010,26(7):680-683
[16]康文艺,张丽,宋艳丽.茜草抑制α-葡萄糖苷酶活性成分研究.中国中药杂志,2009,34(9):1104-1107
[17]潘艺井,蒋文雯,聂昌平,等.7种贵州民族药材提取物对α-葡萄糖苷酶的抑制作用.中华中医药杂志,2012,27(2):385-388
[18]陈平.伏格列波糖片与阿卡波糖片治疗2型糖尿病的疗效及副作用比较.中国现代医生,2012,50(14):73-74
[2]梅兴国.红豆杉细胞培养生产紫杉醇.武汉:华中科技大学出版社,2003:4-5
[3]徐颖扉,张晶,舒琦瑾,等.南方红豆杉水提物诱导人肺腺癌A549细胞凋亡与紫杉醇抑瘤机制不同的研究.中华中医药杂志,2012,27(6):1696-1699
[4]阚常榕,崔炯谟,曹家庆,等.朝鲜产东北红豆杉提取紫杉醇后残液的化学成分研究.中草药,2010,41(11):1785-1787
[5]张丽,李彩芳,李晓梅,等.加拿大蓬α-葡萄糖苷酶抑制作用.河南大学学报:医学版,2008,27(4):39-41
[6]梁敬钰,吴斐华,鲍官虎,等.一种防治糖尿病的植物提取物及其制备方法与药物用途.中国:CN02144302.5.2004-04-14
[7]张学玉,曲玮,梁敬钰.红豆杉属植物化学成分及药理作用研究进展.海峡药学,2011,23(6):5-9
[8]李良松,冯仲科,刘德庆.红豆杉名实与功用通考.中国中药杂志,2011,36(12):1260-1263
[9]张翔宇,杜亚填,龚雪元.南方红豆杉愈伤组织生长动力学及紫杉醇代谢动力学研究.中草药,2012,43(5):990-994
[9]张芳芳,王鹏,姬丹丹,等.南方红豆杉愈伤组织培养条件的优化及紫杉醇积累的基因表达效应分析.中草药,2010,41(12):2058-2062
[11]熊耀康,余陈欢,张春椿,等.高效液相色谱指纹图谱法分析南方红豆杉药材的氯仿提取物析.色谱,2010,28(11):1067-1070
[12]吴慧平,陈建伟,许婷婷,等.骨碎补与夜交藤组合物对α-糖苷酶活性的影响.中国实验方剂学杂志,2012,18(5):181-184
[13]吴慧平,王洪兰,张喆,等.夏枯草迷迭香酸成分分析及其对α-糖苷酶活性影响研究.中药材,2011,34(11):1712-1715
[14]刘新颖,富晓楠,鲍玲,等.楮树皮中生物碱对α-糖苷酶和小肠糖吸收抑制作用研究.中华中医药学刊,2012,30(4):711-712
[15]刘自琴,黄惠华.绿茶和红茶浸提液对α-淀粉酶的抑制作用研究.现代食品科技,2010,26(7):680-683
[16]康文艺,张丽,宋艳丽.茜草抑制α-葡萄糖苷酶活性成分研究.中国中药杂志,2009,34(9):1104-1107
[17]潘艺井,蒋文雯,聂昌平,等.7种贵州民族药材提取物对α-葡萄糖苷酶的抑制作用.中华中医药杂志,2012,27(2):385-388
[18]陈平.伏格列波糖片与阿卡波糖片治疗2型糖尿病的疗效及副作用比较.中国现代医生,2012,50(14):73-74