樟树叶化学成分的研究
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摘要
本文研究了樟树叶的化学成分。主要采用硅胶、Sephadex LH-20凝胶和聚酰胺等柱层析方法对樟树叶的化学成分进行分离纯化,并根据理化性质、波谱数据(1H NMR,13C NMR)鉴定化合物的结构。从樟树叶的甲醇提取物中分离鉴定出9个化合物,分别为山柰酚-3-O-β-D-葡萄糖苷(1)、槲皮素-3-O-β-D-鼠李糖苷(2)、槲皮素-3-O-β-D-葡萄糖苷(3)、异鼠李素-3-O-β-D-葡萄糖苷(4)、黑色五味子单体苷(5)、山奈酚-3-O-β-D-芸香糖苷(6)、异鼠李素-3-O-β-D-芸香糖苷(7)、新芝麻脂素(8)和maculatin(9)。化合物1为首次从樟属中分离得到,化合物4、5、8为首次从樟科植物中分离得到。
To investigate the chemical constituents of the leaves of Cinnamomum camphora. Silica gel,Sephadex LH-20and Polyamide column chromatography were applied for isolation of the constituents. Nine compounds( 1-9) were isolated and their structures were identified by spectroscopic analysis as following: kaempferol-3-O-β-D-glucopyranoside( 1),quercetin-3-O-β-D-rhamnopyranoside( 2),quercetin-3-O-β-D-glucopyranoside( 3),isorhamnetin-3-O-β-D-glucopyranoside( 4),schizandriside( 5),kaempferol-3-O-β-D-rutinoside( 6),isorhamnetin-3-O-β-D-rutinoside( 7),neo-sesamin( 8) and maculatin( 9). Compound 1 was isolated from Cinnamomum spp. for the first time. Compounds 4,5 and 8 were isolated from Lauraceae for the first time.
To investigate the chemical constituents of the leaves of Cinnamomum camphora. Silica gel,Sephadex LH-20and Polyamide column chromatography were applied for isolation of the constituents. Nine compounds( 1-9) were isolated and their structures were identified by spectroscopic analysis as following: kaempferol-3-O-β-D-glucopyranoside( 1),quercetin-3-O-β-D-rhamnopyranoside( 2),quercetin-3-O-β-D-glucopyranoside( 3),isorhamnetin-3-O-β-D-glucopyranoside( 4),schizandriside( 5),kaempferol-3-O-β-D-rutinoside( 6),isorhamnetin-3-O-β-D-rutinoside( 7),neo-sesamin( 8) and maculatin( 9). Compound 1 was isolated from Cinnamomum spp. for the first time. Compounds 4,5 and 8 were isolated from Lauraceae for the first time.
引文
1 Tao GF(陶光复),Phytochemical resources of camphor oils in Hubei,China.Resources and Environment in The Yangtze Basin(长江流域资源与环境),2003,12:124-129.
2 Li AM(李爱民),Tang YQ(唐永勤),Qing YB(卿玉波).Study on the extraction and antimicrobial activity of camphor oil.J Fujian Forestry Sci Tech(福建林业科技),2006,33:121-123.
3 Hu RH(胡仁火),Ren GY(任国祥),Xu H(徐辉),et al.The inhibitory and bactericidal effect of the 8 campus green plants'volatile substances.J Anhui Agri Sci(安徽农业科学),2007,35:9128-9129.
4 Wen CF(文赤夫),Zeng CW(曾超文),Huang ZC(黄早成),et al.The antibacterial activity of the red pigment in the fruit of Cinnamomum camphora.Food and Fermentation Industries(食品发酵与工业),2007,33(4):23-25.
5 Lu LB(卢凌彬),Meng F(孟方).Study on the new fruit and vegetable's preservative.Guangzhou Chem(广州化学),2002,27(3):33-36.
6 Zhou Q(周琼),Liang GW(梁广文).Plant ethanol extraction affects on vegetable aphids and aphid parasitoids.Chin J App Eco(应用生态学报),2003,14:249-252.
7 Yao AQ(姚安庆),Liang DH(梁德华).The biological activity on cabbage butterfly larvae of of the extract from Cinnamomum camphora and Hemerocallis citrina Baroni.Mod Agr(现代农药),2004,3(2):28-29.
8 Lee HJ,Hyun EA,Yoon WJ,et al.In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts.J Ethnopharmacol,2006,103:208-216.
9 Ling J,Liu WY.Cytotoxicity of two new ribosome-inactivating proteins,cinnamomin and camphorin,to carcinoma cells.Cell Biochem Funct,1996,14:157-161.
10 Kim SI,Na YE,Yi JH,et al.Contact and fumigant toxicity of oriental medicinal plant extracts against Dermanyssus gallinae(Acari:Dermanyssidae).Vet Parasitol,2007,145:377-382.
11 El-Shazly AM,Hassan AA,Soliman M,et al.Treatment of human Demodex folliculorum by camphor oil and metronidazole.J Egypt Soc Parasitol,2004,34:107-116.
12 Yi Y(易衍),Wu X(巫鑫),Wang Y(王英),et al.Study on the flavonoids from Rafflesia arnoldii.J Chin Med Mat(中药材),2011,34:712-716.
13 Wang KJ(王开金),Chen LZ(陈列忠),Li N(李宁),et al.The antioxidant and radical scavenging activity of flavonoids from Solidago canadensis.Chin Pharm J(中国药学杂志),2006,41:493-497.
14 Ma L(马麟),Zhou HL(周海玲),Gong YM(龚又明),et al.Study on flavonoids from Artemisia lavandulaefolia.Tradi Chin Dru Rese Clin Phar(中药新药与临床药理),2012,23:555-578.
15 Chen Y(陈雨),Feng X(冯煦),Jia XD(贾晓东),et al.Chemical constituents in the bud of Lonicera macranthoides Hand.-Mazz.Chin Tradit Herb Drugs(中草药),2008,39:823-825.
16 Dong L(董礼),Chen M(陈敏),Li M(李梅),et al.A new cyanogenic glycosides from Rhodiola bupleuroides.Acta Pharmaceutica Sinic,(药学学报),2009,44:1383-1386.
17 Fu W(付伟),Lei YF(雷永芳),Zhou DN(周道年),et al.Study on flavonoids from Parathelypteris nipponica.Chin Pharm J(中国药学杂志),2010,45:166-168.
18 Liu H(刘浩),Zhao JL(赵江林),Lv SQ(吕仕琼),et al.Flavonoids from Halostachys caspica and their antimicrobial and antioxidant activities.中国科技论文在线(http://www.paper.edu.cn)
19 Feng SX(冯世秀),Liu MF(刘梅芳),Wei XY(魏孝义),et al.Study on triterpenoids and flavonoids from Microcos paniculata Linn.J Trop Subtrop Botany(热带亚热带植物学报),2008,16:51-56.
20 Wang Z(王展),He ZS(何直昇).Studies on the chemical constituents of the seeds of Chinese dodder(Cuscuta chinensis).Chin Tradit Herb Drugs(中草药),1998,29:577-579.
21 Chimichi S,Cosirnelli B.Complete1H(600 MHz)and13C NMR Spectral Assignments of 3,4-Bis[(3,4-dimethoxyphenyl)-methyl]dihydro-(3R-trans)-2(3H)-furanone(Matairesinol Dimethyl Ether),a naturally occurring dibenzylbutyrolactone lignan.Magn reson chem,1993,31,1044-1047
22 Liao MC(廖矛川),Yang FY(杨芳云),Sha GP(沙光普),et al.Study on the chemical constituents from the leaves of Cinnamomum camphora.J South-Central Uni.Nat Sci Edi(中南民族大学学报,自科版),2012,31(3):52-55.
2 Li AM(李爱民),Tang YQ(唐永勤),Qing YB(卿玉波).Study on the extraction and antimicrobial activity of camphor oil.J Fujian Forestry Sci Tech(福建林业科技),2006,33:121-123.
3 Hu RH(胡仁火),Ren GY(任国祥),Xu H(徐辉),et al.The inhibitory and bactericidal effect of the 8 campus green plants'volatile substances.J Anhui Agri Sci(安徽农业科学),2007,35:9128-9129.
4 Wen CF(文赤夫),Zeng CW(曾超文),Huang ZC(黄早成),et al.The antibacterial activity of the red pigment in the fruit of Cinnamomum camphora.Food and Fermentation Industries(食品发酵与工业),2007,33(4):23-25.
5 Lu LB(卢凌彬),Meng F(孟方).Study on the new fruit and vegetable's preservative.Guangzhou Chem(广州化学),2002,27(3):33-36.
6 Zhou Q(周琼),Liang GW(梁广文).Plant ethanol extraction affects on vegetable aphids and aphid parasitoids.Chin J App Eco(应用生态学报),2003,14:249-252.
7 Yao AQ(姚安庆),Liang DH(梁德华).The biological activity on cabbage butterfly larvae of of the extract from Cinnamomum camphora and Hemerocallis citrina Baroni.Mod Agr(现代农药),2004,3(2):28-29.
8 Lee HJ,Hyun EA,Yoon WJ,et al.In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts.J Ethnopharmacol,2006,103:208-216.
9 Ling J,Liu WY.Cytotoxicity of two new ribosome-inactivating proteins,cinnamomin and camphorin,to carcinoma cells.Cell Biochem Funct,1996,14:157-161.
10 Kim SI,Na YE,Yi JH,et al.Contact and fumigant toxicity of oriental medicinal plant extracts against Dermanyssus gallinae(Acari:Dermanyssidae).Vet Parasitol,2007,145:377-382.
11 El-Shazly AM,Hassan AA,Soliman M,et al.Treatment of human Demodex folliculorum by camphor oil and metronidazole.J Egypt Soc Parasitol,2004,34:107-116.
12 Yi Y(易衍),Wu X(巫鑫),Wang Y(王英),et al.Study on the flavonoids from Rafflesia arnoldii.J Chin Med Mat(中药材),2011,34:712-716.
13 Wang KJ(王开金),Chen LZ(陈列忠),Li N(李宁),et al.The antioxidant and radical scavenging activity of flavonoids from Solidago canadensis.Chin Pharm J(中国药学杂志),2006,41:493-497.
14 Ma L(马麟),Zhou HL(周海玲),Gong YM(龚又明),et al.Study on flavonoids from Artemisia lavandulaefolia.Tradi Chin Dru Rese Clin Phar(中药新药与临床药理),2012,23:555-578.
15 Chen Y(陈雨),Feng X(冯煦),Jia XD(贾晓东),et al.Chemical constituents in the bud of Lonicera macranthoides Hand.-Mazz.Chin Tradit Herb Drugs(中草药),2008,39:823-825.
16 Dong L(董礼),Chen M(陈敏),Li M(李梅),et al.A new cyanogenic glycosides from Rhodiola bupleuroides.Acta Pharmaceutica Sinic,(药学学报),2009,44:1383-1386.
17 Fu W(付伟),Lei YF(雷永芳),Zhou DN(周道年),et al.Study on flavonoids from Parathelypteris nipponica.Chin Pharm J(中国药学杂志),2010,45:166-168.
18 Liu H(刘浩),Zhao JL(赵江林),Lv SQ(吕仕琼),et al.Flavonoids from Halostachys caspica and their antimicrobial and antioxidant activities.中国科技论文在线(http://www.paper.edu.cn)
19 Feng SX(冯世秀),Liu MF(刘梅芳),Wei XY(魏孝义),et al.Study on triterpenoids and flavonoids from Microcos paniculata Linn.J Trop Subtrop Botany(热带亚热带植物学报),2008,16:51-56.
20 Wang Z(王展),He ZS(何直昇).Studies on the chemical constituents of the seeds of Chinese dodder(Cuscuta chinensis).Chin Tradit Herb Drugs(中草药),1998,29:577-579.
21 Chimichi S,Cosirnelli B.Complete1H(600 MHz)and13C NMR Spectral Assignments of 3,4-Bis[(3,4-dimethoxyphenyl)-methyl]dihydro-(3R-trans)-2(3H)-furanone(Matairesinol Dimethyl Ether),a naturally occurring dibenzylbutyrolactone lignan.Magn reson chem,1993,31,1044-1047
22 Liao MC(廖矛川),Yang FY(杨芳云),Sha GP(沙光普),et al.Study on the chemical constituents from the leaves of Cinnamomum camphora.J South-Central Uni.Nat Sci Edi(中南民族大学学报,自科版),2012,31(3):52-55.