三疣梭子蟹蟹黄油的降血糖作用
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摘要
为研究三疣梭子蟹蟹黄油(egg oil from Portunus trituberculatus,Pt-egg oil)对糖尿病大鼠高血糖症的改善作用及机制,本文采用注射链脲佐菌素的方法建立糖尿病大鼠模型,给予100和400 mg/kg·bw的Pt-egg oil 60 d。实验结束后,检测大鼠空腹血糖和胰岛素水平,检测葡萄糖耐受性,采用实时荧光定量PCR方法检测Pt-egg oil对糖尿病大鼠骨骼肌胰岛素信号通路关键基因m RNA表达水平,采用Western blotting法检测Pt-egg oil对糖尿病大鼠胰岛素信号通路关键蛋白表达的影响。结果显示,经Pt-egg oil饲喂后,大鼠的空腹血糖水平显著地降低了36.52%(p<0.01),血清胰岛素浓度增加了40.21%(p<0.01),葡萄糖耐受性显著改善,骨骼肌磷脂酰肌醇-3-羟激酶(phosphatidylinositol 3-hydroxy kinase,PI3K)、蛋白激酶B(protein kinase B,Akt)和葡萄糖转运蛋白4(glucose transporter 4,Glut4)基因m RNA分别显著增加了114.52%、83.17%和90.06%(p<0.01),蛋白表达分别显著提高了1.54倍、1.61倍和1.37倍(p<0.01),细胞膜上Glut4蛋白表达提高了1.27倍(p<0.01)。表明Pt-egg oil能通过激活PI3K/Akt胰岛素信号通路起到降低糖尿病大鼠血糖的作用。
The aim of the present study is to investigate the effects and mechanism of egg oil from Portunus trituberculatus(Pt-egg oil) on hyperglycaemia improvement in diabetic mice. The diabetes model rats were established by intraperitoneal injection of streptozotocin. The diabetic rats were given Pt-egg oil at 100 and 400 mg/(kg·body weight) for 60 days, respectively. The fasting blood glucose level, serum insulin concentration, and glucose tolerance were determined. The mRNA expression of the key genes in insulin signaling in skeletal muscle of diabetic rats was measured by the quantitative real time PCR assays. The proteins expression levels in insulin signaling in skeletal muscle of diabetic rats was analyzed by Western blotting. Results showed that Pt-egg oil could significantly reduce fasting blood glucose level by 36.52%(p<0.01),increase serum insulin concentration by 40.21%(p<0.01), and improve glucose tolerance. The phosphatidylinositol 3-hydroxy kinase(PI3 K),protein kinase B(Akt), and glucose transporter 4(Glut4) gene mRNA expression in skeletal muscle increased by 114.52%, 83.17%, and 90.06%,respectively. The protein expression of these three genes increased by 1.54-fold, 1.61-fold, and 1.37-fold, respectively. Glut4 protein expression in membrane was also enhanced by 1.27-fold(p<0.01). These indicated that Pt-egg oil might exhibit the effects on hyperglycaemia improvement via activation of PI3 K/Akt insulin signal pathway in diabetic rats.
The aim of the present study is to investigate the effects and mechanism of egg oil from Portunus trituberculatus(Pt-egg oil) on hyperglycaemia improvement in diabetic mice. The diabetes model rats were established by intraperitoneal injection of streptozotocin. The diabetic rats were given Pt-egg oil at 100 and 400 mg/(kg·body weight) for 60 days, respectively. The fasting blood glucose level, serum insulin concentration, and glucose tolerance were determined. The mRNA expression of the key genes in insulin signaling in skeletal muscle of diabetic rats was measured by the quantitative real time PCR assays. The proteins expression levels in insulin signaling in skeletal muscle of diabetic rats was analyzed by Western blotting. Results showed that Pt-egg oil could significantly reduce fasting blood glucose level by 36.52%(p<0.01),increase serum insulin concentration by 40.21%(p<0.01), and improve glucose tolerance. The phosphatidylinositol 3-hydroxy kinase(PI3 K),protein kinase B(Akt), and glucose transporter 4(Glut4) gene mRNA expression in skeletal muscle increased by 114.52%, 83.17%, and 90.06%,respectively. The protein expression of these three genes increased by 1.54-fold, 1.61-fold, and 1.37-fold, respectively. Glut4 protein expression in membrane was also enhanced by 1.27-fold(p<0.01). These indicated that Pt-egg oil might exhibit the effects on hyperglycaemia improvement via activation of PI3 K/Akt insulin signal pathway in diabetic rats.
引文
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[3]Yang Y,Li W,Liu Y,et al.Alpha-lipoic acid attenuates insulin resistance and improves glucose metabolism in high fat diet-fed mice[J].Acta Pharmacologica Sinca,2014,35:1285-1292
[4]农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴2018[M].北京:中国农业出版社,2018
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[7]邵利平,夏文水,姜启兴,等.蟹黄油的理化性质及其营养功能成分分析[J].食品工业科技,2015,36(4):362-369SHAO Li-ping,XIA Wen-shui,JIANG Qi-xing,et al.Analysis of physicochemical properties and nutritive and function al composition of crab oil[J].Science and Technology of Food Industry,2015,36(4):362-369
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[10]Sadeghi A,Djafarian K,Mohammadi H,et al.Effect of omega-3 fatty acids supplementation on insulin resistance in women with polycystic ovary syndrome,Meta-analysis of randomized controlled trials[J].Diabetes and Metabolic Syndrome,2017,11(4-6):157-162
[11]Kwantes JM,Grundmann O.A brief review of krill oil history,research and the commercial market[J].Journal of Dietary Supplements,2015,12(1):23-35
[12]Ivanova Z,Bj?rndal B,Grigorova N,et al.Effect of fish and krill oil supplementation on glucose tolerance in rabbits with experimentally induced obesity[J].Europen Journal of Nutrition,2015,54(10):1055-1067
[13]Voss T S,Vendelbo M H,Kampmann U,et al.Acute hypoglycemia in healthy humans impairs insulin-htimulated glucose uptake and glycogen synthase in skeletal muscle,a randomized clinical study[J].Diabetes,2017,66(9):2483-2494
[14]蔡晟宇.基于IRS-1/PI3K/GLUT4通路观察加味水陆二仙丹对2型糖尿病胰岛素抵抗的影响[D].北京:北京中医药大学,2016CAI Sheng-yu.Effects of Jiawei Shuilu Erxiandan on insulin resistance of type 2 diabetes mellitus through IRS-1/PI3K/GLUT4 pathway[D].Beijing:Beijing University of Chinese Medicine,2016
[15]Zhang M,Li X,Liang H,et al.Semen cassiae extract improves glucose metabolism by promoting Glut4translocation in the skeletal muscle of diabetic rats[J].Frontiers in Pharmacology,2018,9:235
[16]Albers P H,Pedersen A J,Birk J B,et al.Human muscle fiber type-specific insulin signaling,impact of obesity and type 2diabetes[J].Diabetes,2015,64(2):485-497
[17]Gao Y F,Zhang M N,Wang T X,et al.Hypoglycemic effect of D-chiro-inositol in type 2 diabetes mellitus rats through the PI3K/Akt signaling pathway[J].Molecular and Cellular Endocrinology,2016,433(9):26-34
[18]Xiong M,Huang Y,Liu Y,et al.Antidiabetic activity of ergosterol from Pleurotus ostreatus in KK-Ay mice with spontaneous type 2 diabetes mellitus[J].Molecular Nutrition and Food Research,2018,62(3):1700444
[2]Hatem-Vaquero M,Griera M,García-Jerez A,et al.Peripheral insulin resistance in ILK-depleted mice by reduction of GLUT4 expression[J].Journal of Endocrinology,2017,234:115-128
[3]Yang Y,Li W,Liu Y,et al.Alpha-lipoic acid attenuates insulin resistance and improves glucose metabolism in high fat diet-fed mice[J].Acta Pharmacologica Sinca,2014,35:1285-1292
[4]农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴2018[M].北京:中国农业出版社,2018
[5]Pan L,Hu D,Liu M,et al.Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress[J].Gene,2016,576:347-357
[6]Ng'ambi JW,Li R,Mu C,et al.Dietary administration of saponin stimulates growth of the swimming crab Portunus trituberculatus and enhances its resistance against Vibrio alginolyticus infection[J].Fish and Shellfish Immunology,2016,59:305-311
[7]邵利平,夏文水,姜启兴,等.蟹黄油的理化性质及其营养功能成分分析[J].食品工业科技,2015,36(4):362-369SHAO Li-ping,XIA Wen-shui,JIANG Qi-xing,et al.Analysis of physicochemical properties and nutritive and function al composition of crab oil[J].Science and Technology of Food Industry,2015,36(4):362-369
[8]孙甜甜.高品质南极磷虾虾油工业化生产技术研究[D].青岛:中国海洋大学,2013SUN Tian-tian.Studies on the industrialization technology of high quality Antarctic krill oil[D].Qingdao:Ocean University of China,2013
[9]Skorve J,Hilvo M,Vihervaara T,et al.Effects of krill oil on endothelial function and other cardiovascular risk factors in participants with type 2 diabetes,a randomized controlled trial[J].BMJ Open Diabetes Research and Care,2015,3:e000107
[10]Sadeghi A,Djafarian K,Mohammadi H,et al.Effect of omega-3 fatty acids supplementation on insulin resistance in women with polycystic ovary syndrome,Meta-analysis of randomized controlled trials[J].Diabetes and Metabolic Syndrome,2017,11(4-6):157-162
[11]Kwantes JM,Grundmann O.A brief review of krill oil history,research and the commercial market[J].Journal of Dietary Supplements,2015,12(1):23-35
[12]Ivanova Z,Bj?rndal B,Grigorova N,et al.Effect of fish and krill oil supplementation on glucose tolerance in rabbits with experimentally induced obesity[J].Europen Journal of Nutrition,2015,54(10):1055-1067
[13]Voss T S,Vendelbo M H,Kampmann U,et al.Acute hypoglycemia in healthy humans impairs insulin-htimulated glucose uptake and glycogen synthase in skeletal muscle,a randomized clinical study[J].Diabetes,2017,66(9):2483-2494
[14]蔡晟宇.基于IRS-1/PI3K/GLUT4通路观察加味水陆二仙丹对2型糖尿病胰岛素抵抗的影响[D].北京:北京中医药大学,2016CAI Sheng-yu.Effects of Jiawei Shuilu Erxiandan on insulin resistance of type 2 diabetes mellitus through IRS-1/PI3K/GLUT4 pathway[D].Beijing:Beijing University of Chinese Medicine,2016
[15]Zhang M,Li X,Liang H,et al.Semen cassiae extract improves glucose metabolism by promoting Glut4translocation in the skeletal muscle of diabetic rats[J].Frontiers in Pharmacology,2018,9:235
[16]Albers P H,Pedersen A J,Birk J B,et al.Human muscle fiber type-specific insulin signaling,impact of obesity and type 2diabetes[J].Diabetes,2015,64(2):485-497
[17]Gao Y F,Zhang M N,Wang T X,et al.Hypoglycemic effect of D-chiro-inositol in type 2 diabetes mellitus rats through the PI3K/Akt signaling pathway[J].Molecular and Cellular Endocrinology,2016,433(9):26-34
[18]Xiong M,Huang Y,Liu Y,et al.Antidiabetic activity of ergosterol from Pleurotus ostreatus in KK-Ay mice with spontaneous type 2 diabetes mellitus[J].Molecular Nutrition and Food Research,2018,62(3):1700444