结合力生长因子探讨骨肉不相亲理论与骨质疏松症
|
摘要
骨肉不相亲理论是中医学的经典理论,本文基于骨肉不相亲理论,由骨骼肌的机械应力刺激引出力生长因子,从分子生物学的角度论述了骨肉不相亲理论的科学内涵,进一步探讨了骨骼与肌肉的关系,并解释完善了运用骨肉不相亲理论防治骨质疏松症的作用机制,为中医药防治骨质疏松症提供了理论依据。
The theory of disharmony between bone and muscle is a classical theory of traditional Chinese medicine. Based on this theory,this paper draws mechanical growth factor from the mechanical stress stimulation of skeletal muscle and discusses the scientific connotation of this theory from the view of molecular biology. The paper further explores the relationship between the bone and muscle and explains the mechanism of prevention and treatment of osteoporosis using this theory,and provides a theoretical basis for prevention and treatment of osteoporosis with Chinese medicine.
The theory of disharmony between bone and muscle is a classical theory of traditional Chinese medicine. Based on this theory,this paper draws mechanical growth factor from the mechanical stress stimulation of skeletal muscle and discusses the scientific connotation of this theory from the view of molecular biology. The paper further explores the relationship between the bone and muscle and explains the mechanism of prevention and treatment of osteoporosis using this theory,and provides a theoretical basis for prevention and treatment of osteoporosis with Chinese medicine.
引文
[1]张智海,刘忠厚,李娜,等.中国人骨质疏松症诊断标准专家共识(第三稿·2014版).中国骨质疏松杂志,2014,20(9):1007-1010.Zhang ZH,Liu ZH,Li N,et al.Expert consensus on the diagnosis of osteoporosis in Chinese population(the third draft·2014edition).Chin J Osteoporos,2014,20(9):1007-1010.(in Chinese)
[2]Taddei R,Queiroz Junior CM,Moura AP,et al.The effect of CCL3 and CCR1 in bone remodeling induced by mechanical loading during orthodontic tooth movement in mice.Bone,2013,52(1):259-267.
[3]戚孟春,梁永强,孙红,等.张应力下成骨性转录因子在骨髓间充质干细胞中的表达.实用口腔医学杂志,2008(5):654-658.Qi MC,Liang YQ,Sun H,et al.Gene expression of osteogenic transcription factors in rat bone marrow mesenchymal stem cells after a single period of mechanical strain.Journal of Practical Stomatology,2008(5):654-658.(in Chinese)
[4]Haapasalo H,Kontulainen S,Sievanen H,et al.Exercise-induced bone gain is due to enlargement in bone size without a change in volumetric bone density:a peripheral quantitative computed tomography study of the upper arms of male tennis players.Bone,2000,27(3):351-357.
[5]Leblanc A D,Schneider V S,Evans H J,et al.Bone mineral loss and recovery after 17 weeks of bed rest.Journal of Bone and Mineral Research,1990,5(8):843-850.
[6]高平,徐苓,秦明伟,等.骨质疏松与运动的关系——运动员骨密度的横断面研究.中国医学科学院学报,2000(1):61-66.Gao P,Xu L,Qin MW,et al.Bone mineral density and exercises:a cross-sectional study on chinese athletes.Acta Academiae Medicinae Sinicae,2000(1):61-66.(in Chinese)
[7]秦朗.运动对骨密度的影响分析.现代预防医学,2011,10:1886-1887,1889.Qin L.Analysis on the effect of sports on the bone mineral density.Modern Preventive Medicine,2011,10:1886-1887,1889.(in Chinese)
[8]谭思洁,曹立全,王健雄.老年男性肌肉力量、身体成分与骨密度的关联性.中国老年学杂志,2013,01:13-15.Tan SJ,Cao LQ,Wang JX.Association between muscle strength,body composition and bone mineral density of older male.Chinese Journal of Gerontology,2013(1):13-15.(in Chinese)
[9]Mc Koy GL,Ashley W,Mander J,et al.Expression of insulin growth factor-1 splice variants and structural genes in rabbit skeletal muscle induced by stretch and stimulation.J Physiol,1999,516(Pt 2):583-592.
[10]Yang S,Alnaqeeb M,Simpson H,et al.Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch.Muscle Res Cell Motil,1996,17(4):487-495.
[11]Hameed M,Orrell RW,Cobbold M,et al.Expression of IGF-I splice variants in young and old human skeletal muscle after high resistance exercise.Physiol,2003,547(Pt 1):247-254.
[12]张冰玉,宋关斌,罗庆,等.力生长因子的表达及其在组织修复与再生中的作用.中国修复重建外科杂志,2012(5):617-620.Zhang BY,Song GB,Luo Q,et al.Expression of mechano-growth factor and its roles in tissue repairs and regeneration.Chin J Repar Reconst Surg,2012(5):617-620.(in Chinese)
[13]张兵兵,鲜成玉,罗彦凤,等.拉伸刺激下MGF在成骨细胞中的表达及亚细胞定位分析.中国科学(C辑:生命科学),2009(5):518-524.Zhang BB,Xian CY,Luo YF,et al.Expression of MGF in osteoblast under stretching stimulation and subcellular localization analysis.Science in China Serices C,2009(5):518-524.(in Chinese)
[14]Deng M,Zhang B,Wang K,et al.Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits.Int Orthop,2011,35(7):1099-1106.
[15]郭梁.成骨细胞、破骨细胞及OPG/RANKL/RANK轴与骨质疏松症.中医正骨,2010,22(7):41-44,47.Guo L.Association of osteoblast,osteoclast and OPG/RANKL/RANK axis with osteoporosis.The Journal of Traditional Chinese Orthopedics and Traumatology,2010,22(7):41-44,47.(in Chinese)
[16]宋文静,戴钟铨,吴峰,等.力生长因子与成骨细胞.首都师范大学学报(自然科学版),2013,01:41-46.Song WJ,Dai ZQ,Wu F,et al.Relationship between mechanogrowth factor and osteoblast.Journal of Capital Normal University(Natural Science Edition),2013(1):41-46.(in Chinese)
[17]Peng Q,Qiu J,Sun J,et al.The nuclear localization of MGF receptor in osteoblasts under mechanical stimulation.Mol Cell Biochem,2012,369(1-2):147-156.
[18]辛娟,林福春,张兵兵,等.力生长因子E肽对成骨细胞增殖及基因表达的影响.中国生物工程杂志,2011,10:1-6.Xin J,Lin FC,Zhang BB,et al.Effect of MGF-Ct24E on the proliferation of osteoblastic cells and gene expression.J Chin Biotechnol,2011,10:1-6.(in Chinese)
[19]Deng M,Liu P,Xiao H,et al.Improving the osteogenic efficacy of BMP2 with mechano growth factor by regulating the signaling events in BMP pathway.Cell Tissue Res,2015,361(3):723-731.
[20]Xin J,Wang Y,Wang Z,et al.Functional and transcriptomic analysis of the regulation of osteoblasts by mechano-growth factor E peptide.Biotechnol Appl Biochem,2014,61(2):193-201.
[21]Wang C,Meng H,Wang X,et al.Differentiation of bone marrow mesenchymal stem cells in osteoblasts and adipocytes and its role in treatment of osteoporosis.Med Sci Monit,2016,21;22:226-233.
[22]Li H,Lei M,Luo Z,et al.Mechano-growth factor enhances differentiation of bone marrow-derived mesenchymal stem cells.Biotechnol Lett,2015,37(11):2341-2348.
[23]Tong Y,Feng W,Wu Y,et al.Mechano-growth factor accelerates the proliferation and osteogenic differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway.BMC Biochem,2015,16:1.
[24]佟雁翔,冯卫,贾燕飞,等.力生长因子促进骨髓间充质干细胞向成骨细胞的分化.中国组织工程研究,2016,32:4717-4724.Tony YX,Feng W,Jia YF,et al.Mechano growth factor promotes the differentiation of bone marrow mesenchymal stem cells into osteoblasts.J Clin Rehabil Tis Eng Res,2016,32:4717-4724.(in Chinese)
[25]李海滨.力生长因子对骨髓间充质干细胞的生物学作用及机制研究.重庆大学,2015.Li HB.Biological Effects of mechano growth factors on bone marrow mesenchymal stem cells and its mechanism.Chongqing University.2015.(in Chinese)
[26]董万涛,吕泽斌,宋敏,等.“骨肉不相亲”理论发微—从脾肾论治原发性骨质疏松症的科学涵义.中国骨质疏松杂志,2014,20(6):714-717.(in Chinese)Dong WT,Lv ZB,Song M,et al.Scientific meaning of the treatment of primary osteoporosis from the spleen and kidney theory by the divergence of“disharmony between bone and muscle”.Chin J Osteoporos,2014,20(6):714-717.
[2]Taddei R,Queiroz Junior CM,Moura AP,et al.The effect of CCL3 and CCR1 in bone remodeling induced by mechanical loading during orthodontic tooth movement in mice.Bone,2013,52(1):259-267.
[3]戚孟春,梁永强,孙红,等.张应力下成骨性转录因子在骨髓间充质干细胞中的表达.实用口腔医学杂志,2008(5):654-658.Qi MC,Liang YQ,Sun H,et al.Gene expression of osteogenic transcription factors in rat bone marrow mesenchymal stem cells after a single period of mechanical strain.Journal of Practical Stomatology,2008(5):654-658.(in Chinese)
[4]Haapasalo H,Kontulainen S,Sievanen H,et al.Exercise-induced bone gain is due to enlargement in bone size without a change in volumetric bone density:a peripheral quantitative computed tomography study of the upper arms of male tennis players.Bone,2000,27(3):351-357.
[5]Leblanc A D,Schneider V S,Evans H J,et al.Bone mineral loss and recovery after 17 weeks of bed rest.Journal of Bone and Mineral Research,1990,5(8):843-850.
[6]高平,徐苓,秦明伟,等.骨质疏松与运动的关系——运动员骨密度的横断面研究.中国医学科学院学报,2000(1):61-66.Gao P,Xu L,Qin MW,et al.Bone mineral density and exercises:a cross-sectional study on chinese athletes.Acta Academiae Medicinae Sinicae,2000(1):61-66.(in Chinese)
[7]秦朗.运动对骨密度的影响分析.现代预防医学,2011,10:1886-1887,1889.Qin L.Analysis on the effect of sports on the bone mineral density.Modern Preventive Medicine,2011,10:1886-1887,1889.(in Chinese)
[8]谭思洁,曹立全,王健雄.老年男性肌肉力量、身体成分与骨密度的关联性.中国老年学杂志,2013,01:13-15.Tan SJ,Cao LQ,Wang JX.Association between muscle strength,body composition and bone mineral density of older male.Chinese Journal of Gerontology,2013(1):13-15.(in Chinese)
[9]Mc Koy GL,Ashley W,Mander J,et al.Expression of insulin growth factor-1 splice variants and structural genes in rabbit skeletal muscle induced by stretch and stimulation.J Physiol,1999,516(Pt 2):583-592.
[10]Yang S,Alnaqeeb M,Simpson H,et al.Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch.Muscle Res Cell Motil,1996,17(4):487-495.
[11]Hameed M,Orrell RW,Cobbold M,et al.Expression of IGF-I splice variants in young and old human skeletal muscle after high resistance exercise.Physiol,2003,547(Pt 1):247-254.
[12]张冰玉,宋关斌,罗庆,等.力生长因子的表达及其在组织修复与再生中的作用.中国修复重建外科杂志,2012(5):617-620.Zhang BY,Song GB,Luo Q,et al.Expression of mechano-growth factor and its roles in tissue repairs and regeneration.Chin J Repar Reconst Surg,2012(5):617-620.(in Chinese)
[13]张兵兵,鲜成玉,罗彦凤,等.拉伸刺激下MGF在成骨细胞中的表达及亚细胞定位分析.中国科学(C辑:生命科学),2009(5):518-524.Zhang BB,Xian CY,Luo YF,et al.Expression of MGF in osteoblast under stretching stimulation and subcellular localization analysis.Science in China Serices C,2009(5):518-524.(in Chinese)
[14]Deng M,Zhang B,Wang K,et al.Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits.Int Orthop,2011,35(7):1099-1106.
[15]郭梁.成骨细胞、破骨细胞及OPG/RANKL/RANK轴与骨质疏松症.中医正骨,2010,22(7):41-44,47.Guo L.Association of osteoblast,osteoclast and OPG/RANKL/RANK axis with osteoporosis.The Journal of Traditional Chinese Orthopedics and Traumatology,2010,22(7):41-44,47.(in Chinese)
[16]宋文静,戴钟铨,吴峰,等.力生长因子与成骨细胞.首都师范大学学报(自然科学版),2013,01:41-46.Song WJ,Dai ZQ,Wu F,et al.Relationship between mechanogrowth factor and osteoblast.Journal of Capital Normal University(Natural Science Edition),2013(1):41-46.(in Chinese)
[17]Peng Q,Qiu J,Sun J,et al.The nuclear localization of MGF receptor in osteoblasts under mechanical stimulation.Mol Cell Biochem,2012,369(1-2):147-156.
[18]辛娟,林福春,张兵兵,等.力生长因子E肽对成骨细胞增殖及基因表达的影响.中国生物工程杂志,2011,10:1-6.Xin J,Lin FC,Zhang BB,et al.Effect of MGF-Ct24E on the proliferation of osteoblastic cells and gene expression.J Chin Biotechnol,2011,10:1-6.(in Chinese)
[19]Deng M,Liu P,Xiao H,et al.Improving the osteogenic efficacy of BMP2 with mechano growth factor by regulating the signaling events in BMP pathway.Cell Tissue Res,2015,361(3):723-731.
[20]Xin J,Wang Y,Wang Z,et al.Functional and transcriptomic analysis of the regulation of osteoblasts by mechano-growth factor E peptide.Biotechnol Appl Biochem,2014,61(2):193-201.
[21]Wang C,Meng H,Wang X,et al.Differentiation of bone marrow mesenchymal stem cells in osteoblasts and adipocytes and its role in treatment of osteoporosis.Med Sci Monit,2016,21;22:226-233.
[22]Li H,Lei M,Luo Z,et al.Mechano-growth factor enhances differentiation of bone marrow-derived mesenchymal stem cells.Biotechnol Lett,2015,37(11):2341-2348.
[23]Tong Y,Feng W,Wu Y,et al.Mechano-growth factor accelerates the proliferation and osteogenic differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway.BMC Biochem,2015,16:1.
[24]佟雁翔,冯卫,贾燕飞,等.力生长因子促进骨髓间充质干细胞向成骨细胞的分化.中国组织工程研究,2016,32:4717-4724.Tony YX,Feng W,Jia YF,et al.Mechano growth factor promotes the differentiation of bone marrow mesenchymal stem cells into osteoblasts.J Clin Rehabil Tis Eng Res,2016,32:4717-4724.(in Chinese)
[25]李海滨.力生长因子对骨髓间充质干细胞的生物学作用及机制研究.重庆大学,2015.Li HB.Biological Effects of mechano growth factors on bone marrow mesenchymal stem cells and its mechanism.Chongqing University.2015.(in Chinese)
[26]董万涛,吕泽斌,宋敏,等.“骨肉不相亲”理论发微—从脾肾论治原发性骨质疏松症的科学涵义.中国骨质疏松杂志,2014,20(6):714-717.(in Chinese)Dong WT,Lv ZB,Song M,et al.Scientific meaning of the treatment of primary osteoporosis from the spleen and kidney theory by the divergence of“disharmony between bone and muscle”.Chin J Osteoporos,2014,20(6):714-717.