力生长因子与成骨细胞
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摘要
骨组织对所处的力学环境有极强的适应能力.力学因素及非力学因素共同参与调节骨组织的生长、发育与适应性变化,但是关于两者之间的关联调控目前知之甚少.在骨骼肌肉系统中,IGF-I是一种与力刺激密切相关的骨生长因子.在力刺激作用下,IGF-I的pre-mRNA能选择性的剪接成为力生长因子(Mechano-growth factor,MGF),MGF具有促进成肌干细胞和成骨细胞增殖,并抑制其终末分化的作用.在力刺激下,MGF参与骨骼肌肉的重建和修复过程.目前初步研究显示MGF主要分布在细胞核,其信号传导不通过IGF-IR介导.但关于MGF的细胞受体,作用靶点、作用机制和信号传导还未得到有效阐明;本文就MGF与骨骼的力学调控之间的关系进行综述.
Bone has potential adaptive capacity to different mechanical stimulation.The growth,development and adaption of bone were regulated by mechanical factors and nonmechanial factors,but the correlative regulation was poorly understood.IGF is a growth factor which is tightly related with mechanical stimulation in skeletalmuscle system.Pre-mRNA of IGF-I can be alternatively spiliced into mechano-growth factor under mechanical stimulation conditions.MGF could activate satellite cells,promote the proliferation of osteoblast and inhibit their terminal differentiation.Primary studies show that MGF mainly localize in the nuclear and IGF-IR did not mediate the action of MGF.Till now,the receptor,target and mechanism of MGF signaling is not very clear.In present,we summaried the relationship between MGF and mechanical regulaiton of bone.
Bone has potential adaptive capacity to different mechanical stimulation.The growth,development and adaption of bone were regulated by mechanical factors and nonmechanial factors,but the correlative regulation was poorly understood.IGF is a growth factor which is tightly related with mechanical stimulation in skeletalmuscle system.Pre-mRNA of IGF-I can be alternatively spiliced into mechano-growth factor under mechanical stimulation conditions.MGF could activate satellite cells,promote the proliferation of osteoblast and inhibit their terminal differentiation.Primary studies show that MGF mainly localize in the nuclear and IGF-IR did not mediate the action of MGF.Till now,the receptor,target and mechanism of MGF signaling is not very clear.In present,we summaried the relationship between MGF and mechanical regulaiton of bone.
引文
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[30]辛娟,林福春.生长因E肽对成骨细胞增殖及基因表达的影响[J].中国生物工程杂志,2011,31(10):1-6.
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[33]廖保强,邓墨渊,傅亚,等.力生长因子对兔桡骨骨折愈合的作用[J].中国组织工程研究与临床康复,2010(02):245-248.
[34]陈再鸿,李松英,尹美女,等.张应力对骨髓基质细胞分化和力生长因子表达的影响[J].现代口腔医学杂志,2011(03):203-205.
[35]邱敏,李大军,唐丽灵.力生长因子对成骨细胞增殖和分化行为的影响[J].医用生物力学,2009(S1):87-88.
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[37]李大军.力信号调控的大鼠成骨细胞力生长因子表达及其对成骨细胞生理行为的影响[D].重庆大学硕士论文.2008.
[38]Tan D S,Cook A,Chew S L.Nucleolar localization of an isoform of the IGF-I precursor[J].BMC Cell Biol,2002,3:1-6.
[39]Reimers,et al.NoBP,a nuclear fiboblast growth foctor3bindmg protem is cell cycle regulated and promotes cell regulated and promotes cell growth[J].Md Cell Biol,2001,21:4996-5007.
[2]高书明.防骨质疏松运动比补钙更重要[J].中国保健营养,2004(9):54-56.
[3]Dai Z Q,Wu F.IGF-IEc expression,regulation and biological function in different tissues[J].Growth Hormone and IGF Research,2010(20):275-281.
[4]Cowin S C,Hegedus D H.Bone remodeling I:Theory of adaptive elasticity[J].Elasticity,1976(6):313-326.
[5]Hegedus D H,Cowin S C.Bone remodeling II:Small strain adaptive elasticity[J].Elasticity,1976(6):337-352.
[6]Cowin S C,Nachlinger R R.Bone remodeling III.Uniqueness and stability in adaptive elasticity theory[J].Elasticity,1978(8):285-295.
[7]Carter D R.Mechanical loading histories and cortical bone remodeling[J].Calcif Tissue Int,1984,36:S19-S24.
[8]Carter D R.Mechanical loading histories and skeletal biology[J].Biomech,1987,20:1095-1109.
[9]Carter D R,Orr T E,Fyhrie D P,et al.Fluences of mechanical stress on prenatal and postnatal skeletal development[J].Clin Orthop,1987,219:237-250.
[10]Carter D R,Orr T E,Fyhrie D P.Relationships between loading history and femoral cancellous bone architecture[J].Biomech,1989,22:231-234.
[11]王常德.流体剪切力对成骨细胞影响的研究进展[J].临床骨科杂志,2009.12(3):4.
[12]胡颖,王小平.组织工程骨的研究及应用现状[J].口腔领面外科杂志.2004,14(4):367-369.
[13]Nomura T,Mochida J,Okuma M,et al.Nucleus pulposus allograft retards intervertebral disc degeneration[J].Clin Orthop Relat Res,2001,389:94-101.
[14]谢利娜,潘巨利.骨生长因子的研究进展[J].北京口腔医学,2009,17(2):115-117.
[15]Molinelli A,Bonsignore A,Cicconi M,et al.IGF-1abuse in sport:clinical and medicolegal aspects[J].J Sports Med Phys Fitness,2010,50(4):530-535.
[16]Klapper D G,et al.Endoerinology[J].1983:2112-2215.
[17]Heymann G D.Groth hormone stimulatory effects on osteoclastic resorption are partly mediated by insulin-like growth factor-1:an in vitro study[J].Bone,1998,8(4):22-25.
[18]Murphy M,Bach M A,Plotkin D,et al.Oral administra of the growth hormone secretagogue MK-677in2creases markers of bone turnover in healthy and functional2ly impaired elderly adults.The MK-677Study Group[J].J Bone Miner Res,1999,14(7):182-188.
[19]Himpe E,Kooijman R.Insulin-like growth factor-I receptor signal transduction and the Janus Kinase/Signal Transducer and Activator of Transcription(JAK-STAT)pathway[J].Biofactors.2009;35(1):76-81.PMID:19319849.
[20]Goldspink G,Harridge S D.Growth factors and muscle ageing[J].Exp Gerontol,2004(39):52-58.
[21]Bell G I,Stempien M M,Fong N M,et al.Sequences of liver cDNAs encoding two different mouse insulin—like growth factor I precursors[J].Nucleic Acids Res,1986,14:7873-7882.
[22]Yang S,Alnaqeeb M,Simpson H,et al.Cloning and characterlzation of an IGF-1isoform expressed in skeletal muscle subjected to stretch[J].Muscle Res Cell Motil,1996,17:487-495.
[23]Tobin G,Yee D,Brünner N,et al.A novel human insulin-likegrowth factor I messenger RNA is expressed in normal and tumor cells[J].Mol Endocrinol,1990,4(12):1914-1920.
[24]Jansen E,Steenbergh P H,LeRoith D,et al.Identification of multiple transcription start sites in the human insulin-like growth factor-I gene[J].Mol Cell Endocrinol,1991,78(1～2):115-125.
[25]罗宗键.BMP-2和IGF-1共表达载体的构建及其转染对大鼠骨髓间充质干细胞成骨的影响[D].吉林:吉林大学,2008.
[26]Cheema U,Brown R,Mudera V,et al.Mechanical signals and IGF-I gene splicing in vitro in relation to development of skeletal mus-cle[J].Cell Physiol,2005,202(1):67-75.
[27]张兵兵,鲜成玉.拉伸刺激下MGF在成骨细胞中的表达及亚细胞定位分析[J].中国科学C辑:生命科学,2009(39):518～524.
[28]Lean J M.,Jagger C J,Chambers T J,et al.Increased insulin-like growth factor I mRNA expression in rat osteocytes in response to mechanical stress[J].Am J Physiol,1995,268(2pt1):E318-327.
[29]Tokimasa C,Kawata T,Fujita T,et al.Effects of insulin-like growth factor-I on the expression of osteoclasts and osteoblasts in the nasoprema xillary suture under different masticatory loading conditions in growing mice[J].Arch Oral Biol,2003,48(1):31～38.
[30]辛娟,林福春.生长因E肽对成骨细胞增殖及基因表达的影响[J].中国生物工程杂志,2011,31(10):1-6.
[31]鲜成玉,王远亮,张兵兵,等.应力作用下成骨细胞内IGF-I的剪接变异及表达[J].科学通报,2006,51(20):2399—2403.
[32]张兵兵,王远亮,等.力生长因子及其E肽对成骨细胞分化的影响[J].生物化学与生物物理进展,2010(03):304-312.
[33]廖保强,邓墨渊,傅亚,等.力生长因子对兔桡骨骨折愈合的作用[J].中国组织工程研究与临床康复,2010(02):245-248.
[34]陈再鸿,李松英,尹美女,等.张应力对骨髓基质细胞分化和力生长因子表达的影响[J].现代口腔医学杂志,2011(03):203-205.
[35]邱敏,李大军,唐丽灵.力生长因子对成骨细胞增殖和分化行为的影响[J].医用生物力学,2009(S1):87-88.
[36]张兵兵,江鹏,等.力生长因子在大肠杆菌中的表达及活性分析[J].生物工程学报,2008,24(7):1180-1185.
[37]李大军.力信号调控的大鼠成骨细胞力生长因子表达及其对成骨细胞生理行为的影响[D].重庆大学硕士论文.2008.
[38]Tan D S,Cook A,Chew S L.Nucleolar localization of an isoform of the IGF-I precursor[J].BMC Cell Biol,2002,3:1-6.
[39]Reimers,et al.NoBP,a nuclear fiboblast growth foctor3bindmg protem is cell cycle regulated and promotes cell regulated and promotes cell growth[J].Md Cell Biol,2001,21:4996-5007.