黄芩苷对CIA大鼠足关节中炎症因子表达的影响
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摘要
目的:探讨黄芩苷(Baicalin)治疗大鼠胶原诱导性关节炎(CIA)的可能作用机制。方法:制备CIA大鼠模型,分为正常对照组、CIA模型组、黄芩苷组(50mg·kg~(-1)·d~(-1))、甲氨蝶呤组(0.9mg·kg~(-1)·w~(-1)),观察膝关节切片进行HE染色及病理改变,免疫组化法检测关节中炎症因子TNF-α、IL-17、PGE2的表达。结果:与CIA模型组比较,黄芩苷大鼠组关节破坏程度明显减轻,关节炎症因子TNF-α、IL-17、PGE2表达明显降低(P<0.01)。结论:黄芩苷能明显减少CIA大鼠关节中相关炎症因子的表达,减轻关节病理损伤。
Objective:To investigate the possible mechanisms of Baicalin on treating Collagen-induced arthritis in rats.Methods:CIA models were duplicated by SD rats using bovine collagen type 2,then rats were divided into control group,CIA group,baicalin group(50 mg·kg~(-1)·d~(-1))and methotrexate group(0.9 mg·kg~(-1)·w~(-1))randomly.Rats in each gourp recieve appropriate drug administration.The rats were sacrificed after 35 days administration,joints slice were made to get HE staining and immunohistochemical staining.The pathological changes of the cartilage and bone were observed and inflammatory cytokines TNF-alpha,IL-17,PGE2 expression on cartilage and bone were measured.Results:Compared with the CIA group,the arthritis factor TNF-alpha,PGE2,IL-17 expression on rats cartilage and bone in Baicalin group were significantly reduced(P <0.01).There is no significant difference between Baicalin gourp and methotrexate goup(P >0.05).Conclusion:Baicalin can significantly reduce the expression of associated inflammatory factors,as well as could reduce joint pathological damage in CIA rats joints.
Objective:To investigate the possible mechanisms of Baicalin on treating Collagen-induced arthritis in rats.Methods:CIA models were duplicated by SD rats using bovine collagen type 2,then rats were divided into control group,CIA group,baicalin group(50 mg·kg~(-1)·d~(-1))and methotrexate group(0.9 mg·kg~(-1)·w~(-1))randomly.Rats in each gourp recieve appropriate drug administration.The rats were sacrificed after 35 days administration,joints slice were made to get HE staining and immunohistochemical staining.The pathological changes of the cartilage and bone were observed and inflammatory cytokines TNF-alpha,IL-17,PGE2 expression on cartilage and bone were measured.Results:Compared with the CIA group,the arthritis factor TNF-alpha,PGE2,IL-17 expression on rats cartilage and bone in Baicalin group were significantly reduced(P <0.01).There is no significant difference between Baicalin gourp and methotrexate goup(P >0.05).Conclusion:Baicalin can significantly reduce the expression of associated inflammatory factors,as well as could reduce joint pathological damage in CIA rats joints.
引文
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[15]胡军林,欧阳桂林,高华利,等.单用重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白及联合甲氨蝶呤对胶原诱导性关节炎大鼠关节骨破坏影响的实验研究[J].中华风湿病学杂志,2011,15(12):843-845
[16]BETTELLI E,CARRIER Y,GAO W,et al.Reciprocal developmental pathways for the generation of pathogenic effector TH17and regulatory T cells[J].Nature,2006,441(7090):235-238.
[17]HONORATI MC,NERI S,CATTINI L,et al.Interleukin-17,a regulator of angiogenic factor release by synovial fibroblasts[J].Osteoarthritis Cartilage,2006,14(4):345-352.
[18]VAN LG,BEYAERT R.Negative regulation of NF-kappaB and its involvement in rheumatoid arthritis[J].Arthritis Res Ther,2011,13(3):221.
[19]SONG S S,ZHANG L L,WEI W.Establishment of animal model of experimental arthritis and research progress of pathological mechanism[J].Chin Pharmacol Bull,2011,27(12):1648-53.
[2]梁永林,柳春,任真.中医治疗类风湿关节炎用药规律研究[J].中华中医药杂志,2013,28(12):3548-3551.
[3]李俊杰,文学平,徐凌云.黄芩苷新剂型及其药理学研究进展[J].中国新药杂志,2017(17):2046-2051
[4]门素珍,刘巍.黄芩苷在心肌纤维化中的作用[J].新医学,2017,48(12):829-832.
[5]王超,聂浩,李侃,等.黄芩苷固体分散体对小鼠D-氨基半乳糖急性肝损伤的保护作用[J].中国中西医结合杂志,2014,34(1):71-74.
[6]姬春香.黄芩外用治疗急性湿疹的疗效观察及其药理分析[J].实用中西医结合临床,2017,17(11):97-98.
[7]葛永亮,陶金华,吴木军.黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究[J].南京中医药大学学报,2017,33(6):613-617
[8]李少滨,方泓,成颜琦,等.黄芩苷、黄芪甲苷对铜绿假单胞菌生物膜的体外干预实验研究[J].上海中医药杂志,2017,51(11):63-67,83.
[9]文学平,刘德俊,裴忆雪,等.黄芩苷抗急性痛风性关节炎的实验研究[J].中药材,2017(8):1953-1956
[10]朱磊,沈洪,顾培青,等.黄芩苷对溃疡性结肠炎模型大鼠炎性反应、凋亡的影响及与PI3K/AKT通路的关系[J].中华中医药杂志,2017,32(9):4001-4004.
[11]张前,杨浩,陈博,等.黄芩苷防治中枢神经系统疾病的研究进展[J].中国药师,2017,20(2):327-331.
[12]金鹏,许海舰,徐宝欣,等.黄芩苷研究现状及其镁盐研究前景[J].中国实验方剂学杂志,2017,23(20):228-234.
[13]孔薇.Ⅱ型胶原诱导关节炎大鼠模型的制备、评价及部分机制的研究[D].合肥:安徽医科大学,2008.
[14]陈纪藩,叶柳忠,陈光星,等.通痹灵对CIA大鼠关节软骨病变影响的实验研究[J].中国中医基础医学杂志,2003(5):32-35.
[15]胡军林,欧阳桂林,高华利,等.单用重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白及联合甲氨蝶呤对胶原诱导性关节炎大鼠关节骨破坏影响的实验研究[J].中华风湿病学杂志,2011,15(12):843-845
[16]BETTELLI E,CARRIER Y,GAO W,et al.Reciprocal developmental pathways for the generation of pathogenic effector TH17and regulatory T cells[J].Nature,2006,441(7090):235-238.
[17]HONORATI MC,NERI S,CATTINI L,et al.Interleukin-17,a regulator of angiogenic factor release by synovial fibroblasts[J].Osteoarthritis Cartilage,2006,14(4):345-352.
[18]VAN LG,BEYAERT R.Negative regulation of NF-kappaB and its involvement in rheumatoid arthritis[J].Arthritis Res Ther,2011,13(3):221.
[19]SONG S S,ZHANG L L,WEI W.Establishment of animal model of experimental arthritis and research progress of pathological mechanism[J].Chin Pharmacol Bull,2011,27(12):1648-53.