尼罗罗非鱼盐碱选育F_3、F_4、F_5代鱼苗盐碱耐受性评估
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摘要
【目的】对尼罗罗非鱼(Oreochromis niloticus)盐碱选育三、四和五代(F_3、F_4和F_5)鱼苗的盐碱耐受性进行评估,以期为盐碱选育尼罗罗非鱼提供基础资料。【方法】采用急性致死试验方法,分别测定了尼罗罗非鱼盐碱选育F_3、F_4和F_5代对盐、碱(NaHCO_3)以及混合盐碱的耐受指标。【结果】在急性致死条件下,F_3、F_4和F_5代鱼苗96 h盐度半致死浓度分别为22.473、24.468、23.833 g/L。96 h碱度半致死浓度分别为8.256、8.443、8.453 g/L。混合盐碱试验中,盐度为10 g/L时,无死亡情况出现;盐度为15 g/L时,96 h碱度半致死浓度分别为4.612、4.538、4.883 g/L;盐度为20 g/L时,96 h碱度半致死浓度分别为1.861、1.875、1.782 g/L。各急性试验下,F_4和F_5的半致死浓度值差异均不显著;急性盐度试验,F_4和F_5盐度半致死浓度显著高于F_3;急性碱度试验和盐碱混合试验,F_4和F_5半致死浓度与F_3差异不显著。【结论】尼罗罗非鱼盐碱选育F_5代鱼苗盐碱耐受性趋于稳定。
[Objective]The salinity and alkalinity tolerance of the third, fourth and fifth generation (F_3, F_4 and F_5) of Nile tilapia(Oreochromis niloticus) was evaluated in order to provide basic data for the salinity and alkalinity breeding of Nile tilapia.[Method]The acute lethal test method was used to determine the tolerance of salt, alkali(NaHCO_3) andsalinityalkalinity to the F_3, F_4 and F_5 generation of Nile tilapia. [Result]The results showed that under the acute lethal condition,the semi-lethal concentrations of 96 h salinity of F_3, F_4 and F_5 generations were 22.473 g/L, 24.468 g/L and 23.833 g/L,respectively; while the semi-lethal concentrations of 96 h alkalinity were 8.256 g/L, 8.443 g/L and 8.453 g/L, respectively.In the salinity-alkalinity experiment, when the salinity was 10 g/L, there was no death. When the salinity was 15 g/L, the semilethal concentrations of 96 h alkalinity were 4.612 g/L, 4.538 g/L and 4.83 g/L, respectively. When the salinity was 20 g/L, the semi-lethal concentrations of 96 h alkalinity were 1.861 g/L, 1.875 g/L and 1.782 g/L, respectively. Under the acute test, there was no significant difference between F_4 and F_5 selected generations in semi-lethal concentrations of salinity, alkalinity and salinity-alkalinity. Under the acute salinity test, salinity semi-lethal concentrations of F_4 and F_5 were significantly higher than those of F_3.Under the acute alkalinity test and the salinity-alkalinity test, the semi-lethal concentrations of F_4 and F_5 were not significantly different from those of F_3.[Conclusion]The results showed that the salt and alkali tolerance of F_5 selected generation of Nile tilapia was stable.
[Objective]The salinity and alkalinity tolerance of the third, fourth and fifth generation (F_3, F_4 and F_5) of Nile tilapia(Oreochromis niloticus) was evaluated in order to provide basic data for the salinity and alkalinity breeding of Nile tilapia.[Method]The acute lethal test method was used to determine the tolerance of salt, alkali(NaHCO_3) andsalinityalkalinity to the F_3, F_4 and F_5 generation of Nile tilapia. [Result]The results showed that under the acute lethal condition,the semi-lethal concentrations of 96 h salinity of F_3, F_4 and F_5 generations were 22.473 g/L, 24.468 g/L and 23.833 g/L,respectively; while the semi-lethal concentrations of 96 h alkalinity were 8.256 g/L, 8.443 g/L and 8.453 g/L, respectively.In the salinity-alkalinity experiment, when the salinity was 10 g/L, there was no death. When the salinity was 15 g/L, the semilethal concentrations of 96 h alkalinity were 4.612 g/L, 4.538 g/L and 4.83 g/L, respectively. When the salinity was 20 g/L, the semi-lethal concentrations of 96 h alkalinity were 1.861 g/L, 1.875 g/L and 1.782 g/L, respectively. Under the acute test, there was no significant difference between F_4 and F_5 selected generations in semi-lethal concentrations of salinity, alkalinity and salinity-alkalinity. Under the acute salinity test, salinity semi-lethal concentrations of F_4 and F_5 were significantly higher than those of F_3.Under the acute alkalinity test and the salinity-alkalinity test, the semi-lethal concentrations of F_4 and F_5 were not significantly different from those of F_3.[Conclusion]The results showed that the salt and alkali tolerance of F_5 selected generation of Nile tilapia was stable.
引文
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[3]史为良.我国某些鱼类对达里湖碳酸盐型半咸水的适应能力[J].水生生物学集刊,2005,7(3):359-369.SHI W L.The adaptability of some fishes in China to carbonate brackish water in Lake Darry[J].Acta Hydrobiologica Sinica,2005,7(3):359-369.
[4]LemariéG,Baroiller J F,Clota F,Lazard J,Dosdat A.A simple test to estimate the salinity resistance of fish with specific application to O.niloticus and S.melanotheron[J].Aquaculture,2004,240(1-4):575-587.doi:10.1016/j.aquaculture.2004.07.014.
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[6]赵岩,吴俊伟,孟森,王燕,吴昊,赵金良.碳酸盐碱度胁迫对尼罗罗非鱼血清pH、游离氨浓度及相关基因表达的影响[J].南方农业学报,2016,47(6):1032-1038.doi:10.3969/j:issn.2095-1191.2016.06.1032.ZHAO Y,WU J W,MENG S,WANG Y,WU H,ZAHO J L.Effects of carbonate alkalinity on serum pH,ammonia concentration and related gene expression in Oreochromis niloticus[J].Journal of Southern Agriculture,2016,47(6):1032-1038.doi:10.3969/j:issn.2095-1191.2016.06.1032.
[7]么宗利,王慧.罗非鱼咸水养殖研究进展[J].海洋渔业,2006,28(3):251-256.doi:10.3969/j.issn.1004-2490.2006.03.014.YAO Z L,WANG H.Advances on the studies of tilapia cultured in saline water[J].Marine Fisheries,2006,28(3):251-256.doi:10.3969/j.issn.1004-2490.2006.03.014.
[8]Alfredo M H,Hector S L.Blood gasometric trends in hybrid-red tilapia Oreochromis niloticus(Linnaeus)×O.mossambicus(Peters)while adapting to increasing salinity[J].Journal of Aquaculture in the Tropics,2002,2:101-112.
[9]叶畹初,陈大刚,马琳.尼罗罗非鱼(Oreochromis nilotica Linn)幼鱼渗透压调节的初步研究[J].中国海洋大学学报:自然科学版,1989,19(2):88-98.doi:10.16441/j.cnki.hdxb.1989.s2.012.YE W C,CHEN D G,MA L.Preliminary studies on osmotic regulation in the juvenile Oreochromis nilotica(Linn)[J].Periodical of Ocean University of China,1989,19(2):88-98.doi:10.16441/j.cnki.hdxb.1989.s2.012.
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