不同饵料对大鲵血清生化、免疫酶和消化酶活性的影响
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摘要
文章研究了不同饵料(冰鲜杂鱼、活鱼饵)对大鲵血清生化指标、免疫酶和消化酶活性的影响,结果表明,冰鲜组大鲵血清甘油三脂(TG)和谷草转氨酶(ALT)均显著高于活鱼组(P<0.05),血清总蛋白(TP)显著低于活鱼组;冰鲜组肝脏超氧化歧化酶(SOD)显著高于活鱼组(P<0.05),血清碱性磷酸酶(ALP)低于活鱼组,血清酸性磷酸酶(ACP)两组间差异不显著(P>0.05);冰鲜组大鲵肠道胰蛋白酶、胃蛋白酶和肠道脂肪酶活性均显著高于活鱼组(P<0.05),两组大鲵肠道淀粉酶活性差异不显著(P>0.05)。冰鲜杂鱼会影响大鲵的肝脏代谢,从而间接影响大鲵的免疫力,但冰鲜杂鱼投喂大鲵消化酶活性相对较高。
This paper studies the effects of different diets(ice fresh fish and live bait respectively)on the serum biochemistry,immuno-enzyme and digestive enzyme activities of giant salamander(Andrias Davidianus). The results indicated that compared with the giant salamander in live bait group,on the aspect of serum biochemistry,the serum triglyceride and glutamic oxalacetic transaminase of giant salamander in ice fresh fish group were signifi cantly higher(P <0.05). On the aspect of serum immuno-enzyme activity,the serum total protein and alkaline phosphatase in ice fresh fi sh group were both significantly lower(P <0.05),the liver superoxide dismutase activity was significantly higher(P <0.05),and there was no signifi cant difference in the serum acid phosphatase activity between the two groups. On the aspect of serum digestive enzyme activity,the intestinal trypsin,lipase and pepsin activity were signifi cantly higher(P <0.05)in ice fresh fish group than that in live bait group,and there was no significant difference in intestinal amylase activity between the two groups. Taken together,although feeding with ice fresh fish will affect the liver metabolism of giant salamander,which may indirectly affect the immunity of giant salamander,the digestive enzymes activity of giant salamander increased after feeding with ice fresh fish.
This paper studies the effects of different diets(ice fresh fish and live bait respectively)on the serum biochemistry,immuno-enzyme and digestive enzyme activities of giant salamander(Andrias Davidianus). The results indicated that compared with the giant salamander in live bait group,on the aspect of serum biochemistry,the serum triglyceride and glutamic oxalacetic transaminase of giant salamander in ice fresh fish group were signifi cantly higher(P <0.05). On the aspect of serum immuno-enzyme activity,the serum total protein and alkaline phosphatase in ice fresh fi sh group were both significantly lower(P <0.05),the liver superoxide dismutase activity was significantly higher(P <0.05),and there was no signifi cant difference in the serum acid phosphatase activity between the two groups. On the aspect of serum digestive enzyme activity,the intestinal trypsin,lipase and pepsin activity were signifi cantly higher(P <0.05)in ice fresh fish group than that in live bait group,and there was no significant difference in intestinal amylase activity between the two groups. Taken together,although feeding with ice fresh fish will affect the liver metabolism of giant salamander,which may indirectly affect the immunity of giant salamander,the digestive enzymes activity of giant salamander increased after feeding with ice fresh fish.
引文
[1]贲玲芝,李亚卉,梁芸芝,等.不同配合饲料与冰鲜鱼的投喂对三疣梭子蟹生长性能和血液生化指标的影响[J].河北渔业,2018,11:13~17.
[2]陈军,邹鹏,王煜恒,等.不同饵料对大鲵稚体生长性能、体组成和消化酶活性的影响[J].动物营养学报,2017,29(10):3726~3736.
[3]范朋.组胺对黑褐新糠虾和中华绒螯蟹生长发育及部分免疫机能的影响[D].上海:上海海洋大学,2011.
[4]胡月.水产品中生物胺的测定及产组胺菌的分离与鉴定[D].厦门:集美大学,2013.
[5]黄旺.黄粉虫在中华倒刺鲃和大鲵饲料中的应用效果评价[D].重庆:西南大学,2018.
[6]李志斐,龚望宝,王金林,等.冰鲜杂鱼和人工配合饲料对大口黑鲈肌肉品质及健康状况影响的评价[J].动物营养学报,2017,29(11):4180~4188.
[7]牟明明,蒋余,罗强,等.配合饲料和冰鲜鲢对大口黑鲈生长、血浆生化指标、抗氧化能力和组织学的影响[J].水产学报,2018,42(9):1408~1416.
[8]牛化欣,雷霁霖,常杰,等.冰鲜野杂鱼和商品饲料对大菱鲆生长、脂质代谢及抗氧化功能的影响[J].动物营养学报,2013,25(11):2696~2704.
[9]孙翰昌.不同规格大鲵消化酶活性的比较研究[J].水产科学,2014,33(7):459~461.
[10]王芳.血液学指标在大鲵虹彩病毒病诊断与免疫评估中的应用[D].武汉:华中农业大学,2013.
[11]王金锋,杨筱珍,吴旭干,等.水体中组织胺对黑褐新糠虾生长与发育的影响[J].海洋渔业,2008,2:157~162.
[12]原居林,辛建美,练青平,等.五种免疫相关酶在不同年龄组中国大鲵组织内的分布及活性比较研究[J].江西农业大学学报,2013,35(4):831~835.
[13]张金彪.常见野杂鱼中的生物胺分析及其对中华绒螯蟹(Eriocheir sinensis)存活、生长和体内累积的影响[D].上海:上海海洋大学,2012.
[14]周亚,王二龙,杜宗君,等.疑似大鲵生物胺中毒综合征防治实例[J].科学养鱼,2014,5:63.
[15]Dsa K M,TriipethiSG. Studies on digestive enzynme activities of fish Ctenopharyngodon idellus[J].Aquaculture,1991,1:21~23.
[2]陈军,邹鹏,王煜恒,等.不同饵料对大鲵稚体生长性能、体组成和消化酶活性的影响[J].动物营养学报,2017,29(10):3726~3736.
[3]范朋.组胺对黑褐新糠虾和中华绒螯蟹生长发育及部分免疫机能的影响[D].上海:上海海洋大学,2011.
[4]胡月.水产品中生物胺的测定及产组胺菌的分离与鉴定[D].厦门:集美大学,2013.
[5]黄旺.黄粉虫在中华倒刺鲃和大鲵饲料中的应用效果评价[D].重庆:西南大学,2018.
[6]李志斐,龚望宝,王金林,等.冰鲜杂鱼和人工配合饲料对大口黑鲈肌肉品质及健康状况影响的评价[J].动物营养学报,2017,29(11):4180~4188.
[7]牟明明,蒋余,罗强,等.配合饲料和冰鲜鲢对大口黑鲈生长、血浆生化指标、抗氧化能力和组织学的影响[J].水产学报,2018,42(9):1408~1416.
[8]牛化欣,雷霁霖,常杰,等.冰鲜野杂鱼和商品饲料对大菱鲆生长、脂质代谢及抗氧化功能的影响[J].动物营养学报,2013,25(11):2696~2704.
[9]孙翰昌.不同规格大鲵消化酶活性的比较研究[J].水产科学,2014,33(7):459~461.
[10]王芳.血液学指标在大鲵虹彩病毒病诊断与免疫评估中的应用[D].武汉:华中农业大学,2013.
[11]王金锋,杨筱珍,吴旭干,等.水体中组织胺对黑褐新糠虾生长与发育的影响[J].海洋渔业,2008,2:157~162.
[12]原居林,辛建美,练青平,等.五种免疫相关酶在不同年龄组中国大鲵组织内的分布及活性比较研究[J].江西农业大学学报,2013,35(4):831~835.
[13]张金彪.常见野杂鱼中的生物胺分析及其对中华绒螯蟹(Eriocheir sinensis)存活、生长和体内累积的影响[D].上海:上海海洋大学,2012.
[14]周亚,王二龙,杜宗君,等.疑似大鲵生物胺中毒综合征防治实例[J].科学养鱼,2014,5:63.
[15]Dsa K M,TriipethiSG. Studies on digestive enzynme activities of fish Ctenopharyngodon idellus[J].Aquaculture,1991,1:21~23.