复合多糖的佐剂效应研究
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摘要
目的:研究复合多糖佐剂对猪O型口蹄疫(FMDV)灭活抗原的佐剂效应。方法:以对猪O型FMDV灭活抗原具有佐剂效应的黄芪多糖(APS)、人参多糖(GPS)、木蟞子多糖(HPS)、茯苓多糖(LBPS)和云芝多糖(YPS)等五种多糖为佐剂成分,以各多糖的最佳佐剂剂量复合成多糖佐剂配方(CPS),以市售油佐剂为阳性对照,PBS为阴性对照,与FMDV灭活抗原配制成疫苗并免疫猪后,测定比较血清中FMDV灭活抗原诱导特异性抗体水平和不同佐剂诱导分泌IL-2、TNF-α、IFN-γ的变化。结果:CPS佐剂组在一免疫后抗体效价高于阴性对照组,与其他阳性对照组无显著差异,在二免疫后抗体效价高于ISA206油佐剂组,与其他阳性对照无显著差异,且抗体水平显著高于阴性对照组。二免后血清中三种细胞因子含量均高于其他试验组。结论:CPS作为佐剂,能显著提高抗体水平(GMT值),同时促进Th1和Th2型免疫应答,CPS作为新型佐剂具有应用价值。
引文
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[7]单铁英,关华,苏安英,等.枸杞多糖对人外周血淋巴细胞免疫调节功能的影响[J].实用医学杂志,2010,26(03):361-363.
[8] Lee Y S, Han O K, Park C W, et al. Pro-inflammatory gene expression and nitric oxide regulation of aquaeous extracted Astragali radix in R AW264.7 macrophage cells[J].J Ethnopharmacol, 2005, 100(3):289-294.
[9] Sun HX, Wang H, Xu HS, et al. Novel polysaccharide adjuvant fromthe roots of Actinidia eriantha with dual Th1 and Th2 potentiating activity[J].Vaccine, 2009,27(30):3984-3991.
[10]崔倩倩,周志勇,刘朝奇,等.植物多糖作为免疫佐剂的研究进展[J].时珍国医国药,2015,26(04):970-972.
[11]金伯泉,曹雪涛.医学免疫学(第6版)[M].北京:人民卫生出版社,2013.
[2] Harandi A, Medaglini D, Shattock R. Vaccine Adjuvants:A priority for vaccine research[J].Vaccine,2010,28(12):2363-2366.
[3] Hunter, R L.Overview of vaccine adjuvants:present and future[J]. Vaccine,2002. 20(3):7-12.
[4] Meng J, Hu X, Shan F, et al. Analysis of maturation of murine den-dritic cells(DCs)induced by purified Ganoderma lucidum polysaccharides(GLPs)[J].Int J Biol Macromol, 2011,49(4):693-699.
[5] Li X, He X, Liu B, et al. Maturation of murine bone marrow-deriveddendritic cells induced by Radix Glycyrrhizae polysaccharide[J]. Molecules, 2012,17(6):6557-6568.
[6] Lai CY,Hung JT,Lin HH,et al. Immunomodulatory and adjuvant activities of a polysaccharide extract of Ganoderma lucidum in vivo and invitro[J].Vaccine,2010, 28(31):4945-4954.
[7]单铁英,关华,苏安英,等.枸杞多糖对人外周血淋巴细胞免疫调节功能的影响[J].实用医学杂志,2010,26(03):361-363.
[8] Lee Y S, Han O K, Park C W, et al. Pro-inflammatory gene expression and nitric oxide regulation of aquaeous extracted Astragali radix in R AW264.7 macrophage cells[J].J Ethnopharmacol, 2005, 100(3):289-294.
[9] Sun HX, Wang H, Xu HS, et al. Novel polysaccharide adjuvant fromthe roots of Actinidia eriantha with dual Th1 and Th2 potentiating activity[J].Vaccine, 2009,27(30):3984-3991.
[10]崔倩倩,周志勇,刘朝奇,等.植物多糖作为免疫佐剂的研究进展[J].时珍国医国药,2015,26(04):970-972.
[11]金伯泉,曹雪涛.医学免疫学(第6版)[M].北京:人民卫生出版社,2013.