低温胁迫对10份鹅观草属野生种质抗寒生理指标的影响
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摘要
为研究低温胁迫对鹅观草属(Roegneria)野生种质抗寒相关生理指标的影响,以采集自青海三江源区的鹅观草属6个野生种10个居群的植物为研究材料,测定其幼苗在-25~5℃低温胁迫下叶绿素(Chl)、可溶性糖(SS)、丙二醛(MDA)和游离脯氨酸(Pro)含量、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性以及细胞膜透性的变化。结果表明,低温胁迫下供试材料细胞膜透性、Chl含量、MDA含量、SS含量、Pro含量以及CAT、POD活性等抗寒生理指标均有显著变化,不同材料间变化幅度不同,并呈现出不同的变化规律,电解质渗出率持续增加,增加幅度为"小-大-小"的"S"曲线;MDA含量先是所有材料一致逐步提高,后是部分材料降低;CAT活性、POD活性、SS含量和Pro含量变化呈现先增加再降低的倒"V"型;9种供试材料的Chl含量持续降低;应用隶属函数法综合评价鹅观草属各材料的抗寒性能,抗寒性由强到弱顺序为玉树鹅观草(Roegneria yushuensis)(玛多)>玉树鹅观草(Roegneria yushuensis)(铁卜加)>玉树鹅观草(Roegneria yushuensis)(兴海)>肃草(R.stricta)(同仁)>曲芒异芒草(R.abolinii var.divaricans)(湟源)>曲芒异芒草(R.abolinii var.divaricans)(泽库)>短柄鹅观草(R.brevipes)(曲麻莱)>紫穗鹅观草(R.purpurascens)(麦秀林场)>短柄鹅观草(R.brevipes)(泽库)>毛穗鹅观草(R.trichospicula)(泽库)。
In order to study the effect of low temperature on the physiological indexes of 10 populations of six wild species belonging to the Roegneria genus in the Three Rivers region of Qinghai,seedlings with four to five leaf stages were used as experimental materials,and the leaf chlorophyll( Chl),soluble sugar( SS),malondialdehyde( MDA),free proline( Pro),catalase( CAT) and peroxidase( POD) activities,and cell membrane permeability were measured under low temperature stress of between-25 and 5 ℃. The results showed that the cold resistance indexes of plant materials,such as Chl content,MDA,SS content,Pro content,and CAT and POD activity were significantly different under low temperature stress compared to the control. Different materials have different change ranges,and present different change rules,that is electrolyte permeability continues increasing by increasing the size of the S curve. The MDA content of the all experimental materials tended to remain consistent first increase,and then decrease. The contents of SS and Pro presented as an inverted V-shape,increasing first and then decreasing. The Chl content of nine materials continued to decrease. Membership function analysis showed the order of cold resistance of the 10 Roegneria germplasms is:Roegneria yushuensis( Maduo) > R. yushuensis( Tiebujia) > R. yushuensis( Xinhai) > R. stricta( Tongren) >R. abolinii var. divaricans( Huangyuan) > R. abolinii var. divaricans( Zeku) > R. brevipes( Qumalai) > R. purpurascens( Maixiu) > R. brevipes( Zeku) > R. trichospicula( Zeku),in order from the strongest to weakest.
In order to study the effect of low temperature on the physiological indexes of 10 populations of six wild species belonging to the Roegneria genus in the Three Rivers region of Qinghai,seedlings with four to five leaf stages were used as experimental materials,and the leaf chlorophyll( Chl),soluble sugar( SS),malondialdehyde( MDA),free proline( Pro),catalase( CAT) and peroxidase( POD) activities,and cell membrane permeability were measured under low temperature stress of between-25 and 5 ℃. The results showed that the cold resistance indexes of plant materials,such as Chl content,MDA,SS content,Pro content,and CAT and POD activity were significantly different under low temperature stress compared to the control. Different materials have different change ranges,and present different change rules,that is electrolyte permeability continues increasing by increasing the size of the S curve. The MDA content of the all experimental materials tended to remain consistent first increase,and then decrease. The contents of SS and Pro presented as an inverted V-shape,increasing first and then decreasing. The Chl content of nine materials continued to decrease. Membership function analysis showed the order of cold resistance of the 10 Roegneria germplasms is:Roegneria yushuensis( Maduo) > R. yushuensis( Tiebujia) > R. yushuensis( Xinhai) > R. stricta( Tongren) >R. abolinii var. divaricans( Huangyuan) > R. abolinii var. divaricans( Zeku) > R. brevipes( Qumalai) > R. purpurascens( Maixiu) > R. brevipes( Zeku) > R. trichospicula( Zeku),in order from the strongest to weakest.
引文
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[7]陈爱葵,韩瑞宏,李东洋,凌连莲,罗惠霞,唐上剑.植物叶片相对电导率测定方法比较研究.广东教育学院学报,2010,30(5):88-91.Chen A K,Han R H,Li D Y,Lin L L,Luo H X,Tang S J.A comparison of two methods for electrical conductivity about plant leaves.Journal of Guangdong Education Institute,2010,30(5):88-91.(in Chinese)
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[9]冯昌军,罗新义,沙伟,王凤国.低温胁迫对苜蓿品种幼苗SOD、POD活性和脯氨酸含量的影响.草业科学,2005,22(6):29-32.Feng C J,Luo X Y,Sha W,Wang F G.Effect of low temperature stress on SOD、POD activity and proline content of alfalfa.Pratacultural Science,2005,22(6):29-32.(in Chinese)
[10]魏臻武,王德贤,贺连昌.超氧化物歧化酶在苜蓿抗寒锻炼过程中的作用.草业科学,2006,23(7):15-18.Wei Z W,Wang D X,He L C.Effect of superoxide dismutase on cold acclimation of alfalfa.Pratacultural Science,2006,23(7):15-18.(in Chinese)
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[16]德英,赵来喜,穆怀彬.30份老芒麦种质材料抗寒性研究.草业科学,2011,28(1):90-93.Deying,Zhao L X,Mu H B.Winter hardiness of 30 germplasm materials of Elymus sibiricus.Pratacultural Science,2011,28(1):90-93.(in Chinese)
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[18]周瑞莲,赵哈林.高寒山区草本植物的保护的系统及其在低温生长中的作用.西北植物学报,2002,22(3):566-573.Zhou R L,Zhao H L.Protecting enzyme system of herbage and its functions in the cold growing process in alpine and cold region.Acta Botanica Boreali-Occidentalia Sinica,2002,22(3):566-573.(in Chinese)
[19]柏晓玲,周青平,陈有军,田莉华,陈仕勇,肖雪君.燕麦幼苗对低温胁迫的响应.草业科学,2016,33(7):1375-1382.Bai X L,Zhou Q P,Chen Y J,Tian L H,Chen S Y,Xiao X J.Responses of oat seedlings to chilling stress.Pratacultural Science,2016,33(7):1375-1382.(in Chinese)
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