添加硫辛酸对焉耆马运动性能、血浆抗氧化能力及代谢指标的影响
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摘要
试验旨在探讨日粮中添加硫辛酸对焉耆马赛后运动性能、血浆抗氧化能力及代谢指标的影响。选取运动成绩、体重、体尺、年龄相近的焉耆马11匹,随机分为3组,分别为:对照组(3匹)、试验Ⅰ组(4匹)、试验Ⅱ组(4匹)。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组分别在基础日粮中添加12.5、25g/(d·匹)的硫辛酸。试验开始第20天对各组马匹进行20km模拟比赛,赛后30min内测定各组试验马匹的体温、脉搏、呼吸,同时采集各试验组马匹血液。结果表明,无论是赛后0min还是赛后20min,试验Ⅰ、Ⅱ组测得的心率均极显著低于对照组(P<0.01);试验Ⅰ组焉耆马血浆中GSH-Px活力比对照组高44.30%(P<0.05);试验Ⅰ、Ⅱ组焉耆马血浆中SOD活力、T-AOC大小分别比对照组高42.33%(P<0.01)、44.72%(P<0.01)和77.41%(P>0.05)、68.62%(P>0.05);试验Ⅰ、Ⅱ组焉耆马血浆中MDA浓度比对照组低41.09%(P<0.05)、23.48%(P>0.05);试验Ⅰ、Ⅱ组焉耆马血浆中NEFA浓度与对照组相比有升高的趋势(P>0.05)。在饲料中添加硫辛酸,可提高焉耆马运动性能,有利于焉耆马运动后生理指标的快速恢复,提高运动后焉耆马的抗氧化能力,减缓焉耆马在运动期间的疲劳症状,相比而言,添加12.5g/(d·匹)硫辛酸组效果较佳。
This study was conducted to the effects of supplemental different levels of lipoic acid on the performance,plasma antioxidant capacity and other metabolic index of Yanqi horse.11 Yanqi horses were randomly divided into three groups according to performance,body weight,body measurement and age.The groups were referred to as the control group(3horses),groupⅠ(4horses)and group Ⅱ(4horses).The control group was fed basal diet.Horses in the groupsⅠ and Ⅱ were fed with diet supplemented with different levels of lipoic acid.All horses were participated in 20 km racing,on the 20 th day.The temperature,the heart rate,the respiratory rate of all horses were measured,30 min after the racing.Simultaneously,blood samples were collected from the jugular vein.The results showed that the heart rate value of the groupsⅠandⅡ were highly significant lower than the control group(P<0.01),0and 20 min after the racing.Compared withthat of the control group,the plasma GSH-Px activity of the groupⅠ increased by 44.30%(P<0.05);In addition,the plasma SOD,T-AOC activity of the groupsⅠandⅡrespectively increased by 42.33%(P<0.01),44.72%(P<0.01)and 77.41%(P>0.05),68.62%(P>0.05);The plasma MDA concentration of the groupsⅠandⅡ decreased by 41.09%(P<0.05)and 23.48%(P>0.05);and there was a tendency that the plasma NEFA concentration of the groupsⅠ andⅡ were higher than that of the control group(P>0.05).The results showed that the performance and plasma antioxidant capacity of Yanqi horse could be improved by the basal diet added different levels of lipoic acid.Also the physiological function of Yanqi horse could be fast recovery after the racing.Moreover,the symptoms of fatigue of Yanqi horse were mitigated during the racing.In contrast,the effect was better by the addition of lipoic acid in diets of 12.5g/(d·horse).
This study was conducted to the effects of supplemental different levels of lipoic acid on the performance,plasma antioxidant capacity and other metabolic index of Yanqi horse.11 Yanqi horses were randomly divided into three groups according to performance,body weight,body measurement and age.The groups were referred to as the control group(3horses),groupⅠ(4horses)and group Ⅱ(4horses).The control group was fed basal diet.Horses in the groupsⅠ and Ⅱ were fed with diet supplemented with different levels of lipoic acid.All horses were participated in 20 km racing,on the 20 th day.The temperature,the heart rate,the respiratory rate of all horses were measured,30 min after the racing.Simultaneously,blood samples were collected from the jugular vein.The results showed that the heart rate value of the groupsⅠandⅡ were highly significant lower than the control group(P<0.01),0and 20 min after the racing.Compared withthat of the control group,the plasma GSH-Px activity of the groupⅠ increased by 44.30%(P<0.05);In addition,the plasma SOD,T-AOC activity of the groupsⅠandⅡrespectively increased by 42.33%(P<0.01),44.72%(P<0.01)and 77.41%(P>0.05),68.62%(P>0.05);The plasma MDA concentration of the groupsⅠandⅡ decreased by 41.09%(P<0.05)and 23.48%(P>0.05);and there was a tendency that the plasma NEFA concentration of the groupsⅠ andⅡ were higher than that of the control group(P>0.05).The results showed that the performance and plasma antioxidant capacity of Yanqi horse could be improved by the basal diet added different levels of lipoic acid.Also the physiological function of Yanqi horse could be fast recovery after the racing.Moreover,the symptoms of fatigue of Yanqi horse were mitigated during the racing.In contrast,the effect was better by the addition of lipoic acid in diets of 12.5g/(d·horse).
引文
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[5]田芳,仲伟.α-硫辛酸的抗氧化和促氧化双向作用[J].环境与职业医学,2008,25(1):90-93.
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[14]Biewenga G P,Haenen G R,Bast A.The pharmacology of the antioxidant lipoic acid[J].Gen Pharmac,1997,29(3):315-331.
[15]李虎.灌服硫辛酸、番茄红素对SD大鼠运动后几种组织及血浆抗氧化能力的影响[D].乌鲁木齐:新疆农业大学,2012.
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[17]Khanna S,Atalay M,Laaksonen D E,et al.Alpha-lipoic acid supplementation:Tissue glutathione homeostasis at test and following exercise[J].J Appl Physiol,1999,86(4):1191-1198.
[18]Kumnran S,Savitha S.L-carnitine and DL-α-lipoic acid reverse the age-related deficit in glutathione redox state in skeletal muscle and heart tissues[J].Mechanisms of Ageing and Development,2004,125(7):507-512.
[19]李奕.α-硫辛酸对耐力训练小鼠运动后谷胱甘肽抗氧化系统的影响[D].武汉:华东师范大学,2006.
[20]李杏,陈小连,朱丽慧,等.硫辛酸对黄羽肉鸡生长性能、抗氧化能力和免疫功能的影响[J].动物营养学报,2012,24(3):515-521.
[21]李建文,王聪,丁斌鹰.茶多酚和硫辛酸的复合物对氧化应激肉鸭抗氧化功能的影响[J].中国饲料,2010,14:32-34.
[22]熊正英,刘海斌,曲洪刚.α-硫辛酸对大强度耐力运动大鼠不同组织NO浓度、NOS活性的影响[J].天津体育学院学报,2008,23(1):31-33.
[23]张燕,丁建国,赵光.运动性疲劳的机制研究进展[J].中国临床康复,2006,10(44):133-136.
[24]李建龙.运动性疲劳的生化机制研究现状[J].山西医科大学学报,2004,35(2):203-204.
[25]Davis J M,Bailey S P.Possible mechanisms of central nervous system fatigue during exercise[J].Med Sci Sports Exerc,1997,29(1):45-57.
[26]Hughes S J,Potter G D,Greene L W,et al.Adaptation of thoroughbred horses in training to a fat supplemented diet[J].Equine Veterinary Journal,1995,27(18):349-352.
[27]Harking J D,Morris G S,Tulley R T,et al.Effect of added dietary fat on racing performance in thoroughbred horses[J].Journal of Equine Veterinary Science,1992,12(2):123-129.
[2]曹海信,赵启权,李世明.生物自由基与运动性疲劳的研究进展[J].四川体育科学,2006,4:33-35.
[3]汤春芳,刘云国.硫辛酸的研究概况[J].中国生化药物杂志,2005,26(1):52-55.
[4]屯妮萨·麦提赛伊迪.不同精粗比日粮、粗料型日粮添喂烟酰胺对绵羊瘤胃微生物代谢的影响[D].乌鲁木齐:新疆农业大学,2012.
[5]田芳,仲伟.α-硫辛酸的抗氧化和促氧化双向作用[J].环境与职业医学,2008,25(1):90-93.
[6]Tanaka K,Watanabe H,Konishi Y,et al.Longitudinal associations between anaerobic threshold and distance running performance[J].Eur J Appl Physiol,1986,55(3):248-252.
[7]Cottin F,Barrey E,Lopes P,et al.Effect of fatigue during five successive heats(800mat high velocity)and recovery runs on heart rate variability in Standard breds[A].Proceeding of the 7th International Conference on Equine Exercise Physiology,Fontainebleau,France[C].2006.
[8]Mckeever H M.The Athletic horse:Principles and practice of equine sports medicine[M].Linda Duncan,1994.
[9]Ambrosio G,Flaherty J T,Duilio C,et al.Oxygen radicals encrated at reflow induce peroxidation of membrane lipids in reperfused hearts[J].J Clin Invest,1991,87(6):2056-2066.
[10]Art T,Lekeux P.Ventilatory and arterial blood gas tension adjustments to strenuous exercise in standardbreds[J].An J Vet Res,1995,56(10):1332-1337.
[11]Franklin S H,Van Erck-Westergren E,Bayly W M.The respiratory responses of healthy horses to exercise[J].Equine Vet J,2012,44:726-732.
[12]Shapiro K,Gong W C.Natural products used for diabetes[J].J Am Pharm Assoc,2002,42(2):217-226.
[13]Morini M,Roccatagliata L,Dell’Eva R,et al.Alphalipoic acid is effective in prevention and treatment of experimental autoimmune encephalomyelitis[J].Journal of Euroimmunology,2004,148(1-2):146-153.
[14]Biewenga G P,Haenen G R,Bast A.The pharmacology of the antioxidant lipoic acid[J].Gen Pharmac,1997,29(3):315-331.
[15]李虎.灌服硫辛酸、番茄红素对SD大鼠运动后几种组织及血浆抗氧化能力的影响[D].乌鲁木齐:新疆农业大学,2012.
[16]Kuppasamy P,Zweier J L.Characterization of free radical generation by xanthine oxidase:Evidence for hydroxyl radical generation[J].J Biol Chem,1989,264(17):9880-9884.
[17]Khanna S,Atalay M,Laaksonen D E,et al.Alpha-lipoic acid supplementation:Tissue glutathione homeostasis at test and following exercise[J].J Appl Physiol,1999,86(4):1191-1198.
[18]Kumnran S,Savitha S.L-carnitine and DL-α-lipoic acid reverse the age-related deficit in glutathione redox state in skeletal muscle and heart tissues[J].Mechanisms of Ageing and Development,2004,125(7):507-512.
[19]李奕.α-硫辛酸对耐力训练小鼠运动后谷胱甘肽抗氧化系统的影响[D].武汉:华东师范大学,2006.
[20]李杏,陈小连,朱丽慧,等.硫辛酸对黄羽肉鸡生长性能、抗氧化能力和免疫功能的影响[J].动物营养学报,2012,24(3):515-521.
[21]李建文,王聪,丁斌鹰.茶多酚和硫辛酸的复合物对氧化应激肉鸭抗氧化功能的影响[J].中国饲料,2010,14:32-34.
[22]熊正英,刘海斌,曲洪刚.α-硫辛酸对大强度耐力运动大鼠不同组织NO浓度、NOS活性的影响[J].天津体育学院学报,2008,23(1):31-33.
[23]张燕,丁建国,赵光.运动性疲劳的机制研究进展[J].中国临床康复,2006,10(44):133-136.
[24]李建龙.运动性疲劳的生化机制研究现状[J].山西医科大学学报,2004,35(2):203-204.
[25]Davis J M,Bailey S P.Possible mechanisms of central nervous system fatigue during exercise[J].Med Sci Sports Exerc,1997,29(1):45-57.
[26]Hughes S J,Potter G D,Greene L W,et al.Adaptation of thoroughbred horses in training to a fat supplemented diet[J].Equine Veterinary Journal,1995,27(18):349-352.
[27]Harking J D,Morris G S,Tulley R T,et al.Effect of added dietary fat on racing performance in thoroughbred horses[J].Journal of Equine Veterinary Science,1992,12(2):123-129.