纳豆芽孢杆菌对绵羊生长性能、消化性能和血清生化指标的影响
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摘要
本试验旨在研究纳豆芽孢杆菌(BSN)对绵羊生长性能、养分表观消化率和血清生化指标的影响。试验采用单因素完全随机试验设计,将体重相近的40只小尾寒羊分为4组,每组10只,每日饲喂在基础饲粮中分别添加0(CK组)、2(A组)、6(B组)和10 g/只(C组) BSN的饲粮。结果表明:1)与CK组相比,A、B和C组的平均日增重(ADG)分别提高33.43(P>0.05)、32.29(P<0.05)和57.15 g/d(P<0.01)。2)各养分表观消化率随饲粮中BSN添加水平的增加有所提高,但组间差异不显著(P>0.05)。3) A、B和C组血清中尿素氮含量分别比CK组降低0.73%、23.44%和30.04%(P>0.05);血清中免疫球蛋白(Ig) A、IgG和IgM含量组间差异不显著(P>0.05)。在本试验条件下,绵羊饲粮中添加BSN对提高饲粮蛋白质的沉积有积极影响,ADG随BSN添加水平的增加显著增加,每日在饲粮中添加10 g/只BSN效果显著。
This experiment was conducted to investigate the effects of Bacillus subtilis natto( BSN) on growth performance,nutrient apparent digestibility and serum biochemical indexes of sheep. Forty small-tailed Han sheep with the similar body weight were randomly assigned to 4 groups with 10 sheep in each group by a single factor completely randomized design. Sheep were fed diets with 0( CK),2( A),6( B) and 10 g/sheep( C)BSN per day,respectively. The results showed as follows: 1) compared with the CK group,the average daily gain( ADG) in groups A,B and C increased by 33. 43( P > 0. 05),32. 29( P < 0. 05) and 57. 15 g/d( P <0.01),respectively. 2) The nutrient apparent digestibility increased with the BSN supplemental level increasing( P>0.05). 3) The serum urea nitrogen content in groups A,B and C was lower than that in group CK by0.73%,23.44% and 30. 04%( P > 0. 05). There was no significant effect on immune globulin( Ig) A,IgG and IgM contents in serum among groups( P>0.05). It is concluded that the BSN has a positive effect on increasing deposition of crude protein in diets of sheep,ADG increases with the increasing addition of BSN,and the effect is significant when adding 10 g/sheep BSN per day.[Chinese Journal of Animal Nutrition,2019,31( 6) : 2666-2671]
This experiment was conducted to investigate the effects of Bacillus subtilis natto( BSN) on growth performance,nutrient apparent digestibility and serum biochemical indexes of sheep. Forty small-tailed Han sheep with the similar body weight were randomly assigned to 4 groups with 10 sheep in each group by a single factor completely randomized design. Sheep were fed diets with 0( CK),2( A),6( B) and 10 g/sheep( C)BSN per day,respectively. The results showed as follows: 1) compared with the CK group,the average daily gain( ADG) in groups A,B and C increased by 33. 43( P > 0. 05),32. 29( P < 0. 05) and 57. 15 g/d( P <0.01),respectively. 2) The nutrient apparent digestibility increased with the BSN supplemental level increasing( P>0.05). 3) The serum urea nitrogen content in groups A,B and C was lower than that in group CK by0.73%,23.44% and 30. 04%( P > 0. 05). There was no significant effect on immune globulin( Ig) A,IgG and IgM contents in serum among groups( P>0.05). It is concluded that the BSN has a positive effect on increasing deposition of crude protein in diets of sheep,ADG increases with the increasing addition of BSN,and the effect is significant when adding 10 g/sheep BSN per day.[Chinese Journal of Animal Nutrition,2019,31( 6) : 2666-2671]
引文
[1]巨向红,谢晓杭,张金燕,等.一株纳豆芽孢杆菌的饲料学特性研究[J].广东海洋大学学报,2015,35(4):87-92.
[2]吉美萍,肖志婷,那日,等.纳豆芽孢杆菌的相关研究进展[J].河南师范大学学报(自然科学版),2016,44(5):83-93.
[3]张锦华,王萍,何后军,等.纳豆提取物和纳豆菌的体外抑菌作用研究[J].江西畜牧兽医杂志,2006(6):8,42.
[4]OSAWA R,MATSUMOTO K.Digestion of staphylococcal enterotoxin by Bacillus natto[J].Antonie Van Leeuw enhoek,1997,71(4):307-311.
[5]黄俊文.益生菌、甘露寡糖对早期断奶仔猪生产性能、细胞免疫和抗氧化机能的影响[C]//第一届中国养猪生产和疾病控制技术大会---2005中国畜牧兽医学会学术年会论文集.北京:中国畜牧兽医学会,2005:5.
[6]INOOKA S,UEHARA S,KIMURA M.The effect of Bacillus natto on the T and B lymphocytes from spleens of feeding chickens[J].Poultry Science,1986,65(6):1217-1219.
[7]YAMANISHI R,HUANG T,TSUJI H,et al.Reduction of the soybean allergenicity by the fermentation w ith Bacillus natto[J].Food Science and Technology International,1995,1(1):14-17.
[8]陈兵,何世山,朱凤香,等.纳豆芽孢杆菌剂对AA鸡生产性能和十二指肠消化酶的影响[J].浙江农业学报,2003,15(5):289-292.
[9]SUMI H.Accumulation of vitamin K(menaquinone-7)in plasma after ingestion of natto and natto bacilli(B.subtilis natto)[J].Food Science and Technology Research,1999,5(1):48-50.
[10]赵会利,曹玉凤,高艳霞,等.纳豆芽孢杆菌对断奶犊牛生长性能和血液生化指标的影响[J].中国畜牧杂志,2013,49(17):66-69.
[11]SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of prew eaning calves[J].Journal of Dairy Science,2010,93(12):5851-5855.
[12]张志军,刘敏,赵正剑,等.饲粮中添加复合益生菌对绵羊瘤胃发酵的影响[J].饲料研究,2012(3):44-47.
[13]张志军,刘敏,赵正剑,等.饲粮中添加复合益生菌对绵羊血液生化指标的影响[J].中国草食动物,2012,32(1):35-38.
[14]蔡涛,赵泽,王坤,等.微生态制剂对肉用羔羊育肥效果的影响[J].草食家畜,2015(4):30-34.
[15]中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国农业出版社,2004.
[16]张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2002.
[17]ADEOLA O.Digestion and balance techniques in pigs[M]//LEWIS A J,SOUTHERN L L.Swine nutrition.Washington,D.C.:CRC Press,2001:903-916.
[18]张海涛,王加启,卜登攀,等.日粮中添加纳豆枯草芽孢杆菌对断奶前犊牛生长性能的影响[J].中国畜牧杂志,2011,47(3):67-70.
[19]邓露芳.日粮添加纳豆枯草芽孢杆菌对奶牛生产性能、瘤胃发酵及功能微生物的影响[D].博士学位论文.北京:中国农业科学院,2009.
[20]栾广春,王加启,卜登攀,等.N1型纳豆芽孢杆菌对泌乳期奶牛产奶量及乳品质的影响[J].中国畜牧兽医,2009,36(10):12-15.
[21]SOGAARD H,DEMARK T S.Microbials for feed:beyond lactic acid bacteria[J].Feed International,1990,4:33-37.
[22]SONENSHEIN A L,HOCH J A,LOSICK R.Bacillus subtilis and other gram-positive bacteria:biochemistry,physiology,and molecular genetics[M].Washington,D.C.:American Society for M icrobiology,1993:1l-13.
[23]谢红兵.复方中草药免疫增强剂对断奶仔猪免疫功能的影响[D].硕士学位论文.福州:福建农林大学,2004:30-31.
[2]吉美萍,肖志婷,那日,等.纳豆芽孢杆菌的相关研究进展[J].河南师范大学学报(自然科学版),2016,44(5):83-93.
[3]张锦华,王萍,何后军,等.纳豆提取物和纳豆菌的体外抑菌作用研究[J].江西畜牧兽医杂志,2006(6):8,42.
[4]OSAWA R,MATSUMOTO K.Digestion of staphylococcal enterotoxin by Bacillus natto[J].Antonie Van Leeuw enhoek,1997,71(4):307-311.
[5]黄俊文.益生菌、甘露寡糖对早期断奶仔猪生产性能、细胞免疫和抗氧化机能的影响[C]//第一届中国养猪生产和疾病控制技术大会---2005中国畜牧兽医学会学术年会论文集.北京:中国畜牧兽医学会,2005:5.
[6]INOOKA S,UEHARA S,KIMURA M.The effect of Bacillus natto on the T and B lymphocytes from spleens of feeding chickens[J].Poultry Science,1986,65(6):1217-1219.
[7]YAMANISHI R,HUANG T,TSUJI H,et al.Reduction of the soybean allergenicity by the fermentation w ith Bacillus natto[J].Food Science and Technology International,1995,1(1):14-17.
[8]陈兵,何世山,朱凤香,等.纳豆芽孢杆菌剂对AA鸡生产性能和十二指肠消化酶的影响[J].浙江农业学报,2003,15(5):289-292.
[9]SUMI H.Accumulation of vitamin K(menaquinone-7)in plasma after ingestion of natto and natto bacilli(B.subtilis natto)[J].Food Science and Technology Research,1999,5(1):48-50.
[10]赵会利,曹玉凤,高艳霞,等.纳豆芽孢杆菌对断奶犊牛生长性能和血液生化指标的影响[J].中国畜牧杂志,2013,49(17):66-69.
[11]SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of prew eaning calves[J].Journal of Dairy Science,2010,93(12):5851-5855.
[12]张志军,刘敏,赵正剑,等.饲粮中添加复合益生菌对绵羊瘤胃发酵的影响[J].饲料研究,2012(3):44-47.
[13]张志军,刘敏,赵正剑,等.饲粮中添加复合益生菌对绵羊血液生化指标的影响[J].中国草食动物,2012,32(1):35-38.
[14]蔡涛,赵泽,王坤,等.微生态制剂对肉用羔羊育肥效果的影响[J].草食家畜,2015(4):30-34.
[15]中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国农业出版社,2004.
[16]张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2002.
[17]ADEOLA O.Digestion and balance techniques in pigs[M]//LEWIS A J,SOUTHERN L L.Swine nutrition.Washington,D.C.:CRC Press,2001:903-916.
[18]张海涛,王加启,卜登攀,等.日粮中添加纳豆枯草芽孢杆菌对断奶前犊牛生长性能的影响[J].中国畜牧杂志,2011,47(3):67-70.
[19]邓露芳.日粮添加纳豆枯草芽孢杆菌对奶牛生产性能、瘤胃发酵及功能微生物的影响[D].博士学位论文.北京:中国农业科学院,2009.
[20]栾广春,王加启,卜登攀,等.N1型纳豆芽孢杆菌对泌乳期奶牛产奶量及乳品质的影响[J].中国畜牧兽医,2009,36(10):12-15.
[21]SOGAARD H,DEMARK T S.Microbials for feed:beyond lactic acid bacteria[J].Feed International,1990,4:33-37.
[22]SONENSHEIN A L,HOCH J A,LOSICK R.Bacillus subtilis and other gram-positive bacteria:biochemistry,physiology,and molecular genetics[M].Washington,D.C.:American Society for M icrobiology,1993:1l-13.
[23]谢红兵.复方中草药免疫增强剂对断奶仔猪免疫功能的影响[D].硕士学位论文.福州:福建农林大学,2004:30-31.