氧化酶法快速测定发酵液中赖氨酸含量的研究
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摘要
为了研究氧化酶法快速测定赖氨酸发酵液中的赖氨酸含量的可行性,借助生物传感分析仪,连续使用酶膜50天的结果表明:膜的活性仍在800 mv以上、酶膜完整、线性(R~2=0. 9997)优良;检测结果的相对偏差、标准误差均优于茚三酮显色法;检测总成本(100批次/天)为11. 40元,低于茚三酮显色法的42. 62元,在大规模生产中监控赖氨酸含量变化可行。
In order to study the feasibility of rapid determination of lysine content in lysine fermentation broth by oxidase method,with the help of biosensor analyzer,the results of continuous application of enzyme membrane for 50 days showed that the membrane activity is still above 800 mv,the enzyme membrane is intact and linear (R~2= 0. 9997) is good. The relative deviation and standard error of the results were better than ninhydrin coloration method. The total cost of detection (100 batches per day) was11. 40 yuan,which was lower than 42. 62 yuan of ninhydrin coloration method. It was feasible to monitor the change of lysine content in large-scale production.
In order to study the feasibility of rapid determination of lysine content in lysine fermentation broth by oxidase method,with the help of biosensor analyzer,the results of continuous application of enzyme membrane for 50 days showed that the membrane activity is still above 800 mv,the enzyme membrane is intact and linear (R~2= 0. 9997) is good. The relative deviation and standard error of the results were better than ninhydrin coloration method. The total cost of detection (100 batches per day) was11. 40 yuan,which was lower than 42. 62 yuan of ninhydrin coloration method. It was feasible to monitor the change of lysine content in large-scale production.
引文
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[13]ROMETTE J L,孙泽辉,汪庆松.测定L-赖氨酸的专用酶电极,氨基酸杂志[J].氨基酸和生物资源,1985(2):67-72.
[14]冯东,王丙莲.生物传感器法测定麦芽汁中赖氨酸含量的研究[J].激光生物学报,2014,23(3):270-274.
[15]山东省科学院生物研究所.SBA-40D生物传感分析仪使用说明[G].
[2]NELOFER R,SYED Q,NADEEM M.Statistical optimization of process variables for L-lysine production by Corynebacteriumglutamicun[J].Turk Biyokimya Dergisi,2008,33(2):50-57.
[3]DEBATOSH D,AKSHAY B,VIDYA C.Lysine:Is it worth more?[J].Cytotechnology,2001(1):3-32.
[4]OH J W,KIM S J,CHO Y J,et al.Strain of Corynebacterium Glutamicum and Method for Producing L-lysine:US,5268293[P].1993-12-07.
[5]NAKAZAWA M,TAKAHASHI D,ONISHI N,et al.L-Lysinefermentation:us,20067012152[P].2006.
[6]ZELDER O,KLOPPROGGE C,SCHONER H,et al.L-lysine fermentation[J].Microbial Technol.,2005,1(1):211-240.
[7]RAO D,RAZAK S,PRAVEENA B,et al.Dissolved oxygen concentration analysis of L-Lysine fermentation production by Corynebacterium glutamicum[J].Internet Journal of Pharmacology,2008,6(1),DOI:10.5580/1910
[8]ANASTASSIADIS S.L-Lysine fermentation[J].Microbial Technolol.,2007,1(1):11-24.
[9]杨杨,刘永成,刘利群,等.HPLC法测定葡萄糖酸钙锌口服溶液中盐酸赖氨酸含量[J].黑龙江医药,2014(3):489-491.
[10]张书贤.茚三酮比色法测饲料中赖氨酸含量研究[J].四川畜牧兽医,1995(1):34-35.
[11]李宪民,丁芳,樊伟丽,等.生物传感分析仪测定葡萄糖和L-乳酸的影响因素研究[J].亚太传统医药,2008(9):5-7。
[12]史建国,马耀宏,孟庆军,等.氨基酸有机酸发酵过程中的生物传感器[J].发酵科技通讯,2009,38(4):47-49.
[13]ROMETTE J L,孙泽辉,汪庆松.测定L-赖氨酸的专用酶电极,氨基酸杂志[J].氨基酸和生物资源,1985(2):67-72.
[14]冯东,王丙莲.生物传感器法测定麦芽汁中赖氨酸含量的研究[J].激光生物学报,2014,23(3):270-274.
[15]山东省科学院生物研究所.SBA-40D生物传感分析仪使用说明[G].