低频磁场对紫色红曲菌液态发酵产麦角固醇的影响
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摘要
探讨低频磁场处理对紫色红曲菌(Monascus purpurcus)发酵产麦角固醇的影响,确定最佳处理条件。分别研究低频磁场强度、处理时间和处理时期对紫色红曲霉液态发酵产麦角固醇的影响规律,探讨在最佳处理条件下,磁场对紫色红曲霉麦角固醇代谢过程的影响和紫色红曲霉的生物量在整个发酵周期的变化,及对紫红曲霉其他主要代谢物的影响。结果表明:低频磁场处理紫色红曲菌的最适条件为磁场强度1.2 mT、处理时间2 d、处理时期在发酵0~2 d,在发酵第12天,麦角固醇产量达到了7.08 mg/g,生物量达到3.2 g/L,比对照组分别增加了62.5%和31.68%。低频磁场是提高紫色红曲菌生产麦角固醇产量的有效手段,研究为低频磁场辅助微生物发酵,提高有益产物的合成提供了数据支持。
We explored the effects of low-frequency magnetic field(LF-MF) on the production of ergosterol by fermentation with Monascus purpurcus and further determined the optimal LF-MF treatment conditions such as LF-MF intensity, time treatment time and the fermentation stage when M. purpurcus was treated with LF-MF on ergosterol production by liquid-state fermentation of M. purpurcus. We also investigated the effects of LF-MF on the metabolism of ergosterol by M. purpurcus, and examined the change in M. purpurcus biomass throughout the fermentation period and the effects on other main metabolites of M. purpurcus under optimal treatment conditions. Results showed that ergosterol yield was 7.08 mg/g and the biomass of M. purpurcus was 3.2 g/L on the 12~(th) day of fermentation, which were respectively increased by 62.5% and 31.68% as compared to that of the control when M. purpurcus was treated by LF-MF at an intensity of 1.2 m T during the first two days of fermentation. Consequently, LF-MF was an effective method to enhance ergosterol yield. This result may provide evidence supporting the use of LF-MF to improve the production of beneficial microbial metabolites by fermentation.
We explored the effects of low-frequency magnetic field(LF-MF) on the production of ergosterol by fermentation with Monascus purpurcus and further determined the optimal LF-MF treatment conditions such as LF-MF intensity, time treatment time and the fermentation stage when M. purpurcus was treated with LF-MF on ergosterol production by liquid-state fermentation of M. purpurcus. We also investigated the effects of LF-MF on the metabolism of ergosterol by M. purpurcus, and examined the change in M. purpurcus biomass throughout the fermentation period and the effects on other main metabolites of M. purpurcus under optimal treatment conditions. Results showed that ergosterol yield was 7.08 mg/g and the biomass of M. purpurcus was 3.2 g/L on the 12~(th) day of fermentation, which were respectively increased by 62.5% and 31.68% as compared to that of the control when M. purpurcus was treated by LF-MF at an intensity of 1.2 m T during the first two days of fermentation. Consequently, LF-MF was an effective method to enhance ergosterol yield. This result may provide evidence supporting the use of LF-MF to improve the production of beneficial microbial metabolites by fermentation.
引文
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[19]万云雷,韩红霞,李利,等.低频磁场对紫色红曲菌固态发酵产γ-氨基丁酸的影响[J].中国农业科技导报,2015,17(5):94-98.DOI:10.13304/j.nykjdb.2015.478.
[20]李利.红色红曲菌G蛋白信号途径相关基因的克隆及功能研究[D].武汉:华中农业大学,2011.
[21]曾冬杰.紫红曲霉在低频磁场条件下主要固态发酵产物的代谢规律[D].荆州:长江大学,2013.
[22]NILSSON K,BJURMAN J.Estimation of mycelial biomass by determination of the ergosterol content of wood decayed by coniophora puteana and fomes fomentarius[J].Material Und Organismen,1990,25:275-283.
[23]DAWSON-ANDOH B E.Ergosterol content as a measure of biomass of potential biological control fungi in liquid cultures[J].Holz als Rohund Werkstoff,2002,60:115-117.DOI:10.1007/s00107-001-0274-9.
[24]宁玮霁.红曲霉液态发酵法高产麦角固醇的研究[D].贵阳:贵州大学,2008.
[25]习兴梅,曾光明,郁红艳,等.真菌生物量指示剂麦角固醇的分离及测定方法[J].微生物学通报,2006,33(3):128-132.DOI:10.13344/j.microbiol.china.2006.03.026.
[26]LI L,SHAO Y,LI Q,et al.Identification of Mga1,a G-proteinα-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7[J].Fems Microbiology Letters,2010,308(2):108-114.DOI:10.1111/j.1574-6968.2010.01992.x.
[2]苗景赟,邓利,谭天伟.麦角固醇连续光转化生产维生素D2新工艺[J].北京化工大学学报,2003,30(3):39-41.DOI:10.3969/j.issn.1671-4628.2003.03.010.
[3]韩庆雪.麦角固醇提取工艺的中试放大研究[D].天津:天津大学,2007.
[4]BANAT I M,NIGAM P,SINGH D,et al.Review:ethanol production at elevated temperatures and alcohol concentrations.Ⅰ.Yeasts in general[J].World Journal of Microbiology and Biotechnology,1998,14(6):809-821.DOI:10.1023/A:1008852424846.
[5]惠丰立,魏明卉,褚学英.产麦角固醇酵母菌株的筛选及发酵条件优化[J].中国医药工业杂志,2004,35(3):138-140.DOI:10.3969/j.issn.1001-8255.2004.03.006.
[6]张丽丽,谭艾娟,刘爱英,等.红曲霉中麦角固醇皂化条件的优化[J].山地农业生物学报,2010,29(2):143-146.DOI:10.3969/j.issn.1008-0457.2010.02.011.
[7]孙佰申,周立平,陈旭峰,等.HPLC法测定红曲霉发酵样品中麦角固醇的含量[J].中国食品添加剂,2004(2):89-92.DOI:10.3969/j.issn.1006-2513.2004.02.023.
[8]张鹏荣.产黄青霉固体发酵产麦角固醇的研究[D].西安:西安建筑科技大学,2009.DOI:10.7666/d.d193796.
[9]柯亚萍.黑曲霉固体发酵产麦角固醇的研究[D].西安:西安建筑科技大学,2008.DOI:10.7666/d.d194634.
[10]谢宗良.产麦角固醇酵母菌的筛选、诱变与发酵条件优化[D].保定:河北大学,2011.DOI:10.7666/d.y1634619.
[11]宁玮霁,谭艾娟,刘爱英,等.红曲霉产麦角固醇液体培养基筛选[J].山地农业生物学报,2008,27(3):241-246.DOI:10.3969/j.issn.1008-0457.2008.03.011.
[12]谭艾娟,宁玮霁,刘爱英,等.红曲霉产麦角固醇液体发酵条件优化[J].食品科学,2008,29(9):434-436.DOI:10.3321/j.issn:1002-6630.2008.09.099.
[13]ALVAREZ D A,PEREZ V H,JUSTO O R,et al.Effect of the extremely low frequency magnetic field on nisin production by Lactococcus lactis subsp.lactisusing cheese whey permeate[J].Process Biochemistry,2006,41:1967-1973.DOI:10.1016/j.procbio.2006.04.009.
[14]PEREZ V H,REYES A F,JUSTO O R,et al.Bioreactor coupled with electromagnetic field generator:effects of extremely low frequency electromagnetic fields on ethanol production by Saccharomyces cerevisiae[J].Biotechnology Progress,2007,23:1091-1094.DOI:10.1021/bp070078k.
[15]CANLI O,ERDAL S,TASKIN M,et al.Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis[J].Toxicology and Industrial Health,2011,27:35-39.DOI:10.1177/0748233710380219.
[16]GAO M X,ZHANG J L,FENG H.Low-frequency magnetic field effects on metabolite of Aspergillus niger[J].Bioelectromagnetics,2011,32:73-78.DOI:10.1002/bem.20619.
[17]邓光武,夏帆,王洁雅,等.低频交变磁场对红曲霉固态发酵生物量的影响[J].农业机械学报,2012,43(6):128-132.DOI:10.6041/j.issn.1000-1298.2012.06.024.
[18]ZHANG J L,ZENG D J,XU C,et al.Effect of low-frequency magnetic field on formation of pigments of Monascus purpureus[J].European Food Research and Technology,2015,240:577-582.DOI:10.1007/s00217-014-2358-x.
[19]万云雷,韩红霞,李利,等.低频磁场对紫色红曲菌固态发酵产γ-氨基丁酸的影响[J].中国农业科技导报,2015,17(5):94-98.DOI:10.13304/j.nykjdb.2015.478.
[20]李利.红色红曲菌G蛋白信号途径相关基因的克隆及功能研究[D].武汉:华中农业大学,2011.
[21]曾冬杰.紫红曲霉在低频磁场条件下主要固态发酵产物的代谢规律[D].荆州:长江大学,2013.
[22]NILSSON K,BJURMAN J.Estimation of mycelial biomass by determination of the ergosterol content of wood decayed by coniophora puteana and fomes fomentarius[J].Material Und Organismen,1990,25:275-283.
[23]DAWSON-ANDOH B E.Ergosterol content as a measure of biomass of potential biological control fungi in liquid cultures[J].Holz als Rohund Werkstoff,2002,60:115-117.DOI:10.1007/s00107-001-0274-9.
[24]宁玮霁.红曲霉液态发酵法高产麦角固醇的研究[D].贵阳:贵州大学,2008.
[25]习兴梅,曾光明,郁红艳,等.真菌生物量指示剂麦角固醇的分离及测定方法[J].微生物学通报,2006,33(3):128-132.DOI:10.13344/j.microbiol.china.2006.03.026.
[26]LI L,SHAO Y,LI Q,et al.Identification of Mga1,a G-proteinα-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7[J].Fems Microbiology Letters,2010,308(2):108-114.DOI:10.1111/j.1574-6968.2010.01992.x.