HPLC双波长法同时测定桦褐孔菌子实体中麦角甾醇和麦角甾酮的含量
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摘要
目的建立高效液相色谱双波长法同时测定桦褐孔菌中麦角甾醇和麦角甾酮的含量。方法采用的色谱柱为岛津Inertsil ODS-SP C_(18)柱(4.6 mm×250 mm,5μm);流动相为甲醇;流速为1 mL·min~(-1);检测波长为282nm、349 nm;柱温为30℃。结果麦角甾醇和麦角甾酮的进样量分别在0.404~1.616μg(r=1)、19.88~139.16 ng(r=1)范围内呈良好线性关系,平均回收率分别为99.32%、99.34%,RSD分别为0.92%、1.02%。结论所建立的方法准确可靠、重复性好,可用于桦褐孔菌中麦角甾醇、麦角甾酮化学成分的含量测定,为控制该药物的质量提供参考。
Objective To establish an HPLC method with dual wavelength detection for the determination of ergosterol and ergot sterone in Inonotus obliquus.Methods Analysis were performed on a Inertsil ODS-SP C_(18)column( 4.6 mm× 250 mm,5 μm); the mobile phase was methanol with the flow rate at 1 mL·min~(-1); the detection wavelength was 282 nm,349 nm and the column temperature was 30 ℃.Results The linear ranges of ergosterol and ergot sterone were 0.404 ~ 1.616 μg( r= 1),19.88 ~ 139.16 ng( r = 1),respectively,and the average recoveries were 99.32% and 99.34% with the RSDs of 0.92% and 1.02%.Conclusion The method was simple and accurate with a good reproducibility,it can be used for the determination of ergosterol,ergot sterone in Inonotus obliquus to control the quanlity of drug.
Objective To establish an HPLC method with dual wavelength detection for the determination of ergosterol and ergot sterone in Inonotus obliquus.Methods Analysis were performed on a Inertsil ODS-SP C_(18)column( 4.6 mm× 250 mm,5 μm); the mobile phase was methanol with the flow rate at 1 mL·min~(-1); the detection wavelength was 282 nm,349 nm and the column temperature was 30 ℃.Results The linear ranges of ergosterol and ergot sterone were 0.404 ~ 1.616 μg( r= 1),19.88 ~ 139.16 ng( r = 1),respectively,and the average recoveries were 99.32% and 99.34% with the RSDs of 0.92% and 1.02%.Conclusion The method was simple and accurate with a good reproducibility,it can be used for the determination of ergosterol,ergot sterone in Inonotus obliquus to control the quanlity of drug.
引文
[1]黄年来.俄罗斯神秘的民间药用真菌—桦褐孔菌[J].中国食用菌,2002,21(4):7-8.
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[9]相聪坤,宿树兰,关胜,等.HPLC法测定不同产地不同品种马勃药材中麦角甾醇[J].中草药,2016,47(8):1397-1400.
[10]李思明,冯怡,曾星.HPLC-APCI-MS/MS法同时测定猪苓颗粒中麦角甾酮与麦角甾醇的含量[J].药物分析杂志,2014,34(4):649-653.
[11]赵英永,程显隆,张萍,等.HPLC法测定猪苓中麦角甾酮的含量[J].药物分析杂志,2009,29(9):1579-1581.
[2]杜文婧,王琦.桦褐孔菌资源分布及药理活性研究[J].菌物研究,2013,11(1):49-56.
[3]曾小龙.桦褐孔菌的化学成分与药理作用研究[J].广东教育学院学报,2007,27(3):76-81.
[4]RZYMOWSKA J.The effect of aqueous extracts from Inonotus obliquus on the mitotic index and en-zyme activities[J].Boll Chin Farm,1998,137(1):13-15.
[5]SHIN Y,TAMAI Y,TERAZAWA M.Chemical constituents of Inonotus obliquusⅢ:3β,22,25-tri-hydroxy-lanosta-8-ene form scretium[J].Int J Med Mushrooms,2001,3(2):201-206.
[6]SHIN Y,TAMAI Y,TERAZAWA M.Chemical constituents of Inonotus obliquusⅣ-triterpene and steroids from cultured mycelia[J].Eurasian J Forest Res,2001(2):27-34.
[7]赵芬琴,朴惠善.桦褐孔菌的化学成分研究[J].时珍国医国药,2006,17(7):1178-1181.
[8]何坚,冯孝章.桦褐孔菌化学成分的研究[J].中草药,2001,32(1):4-6.
[9]相聪坤,宿树兰,关胜,等.HPLC法测定不同产地不同品种马勃药材中麦角甾醇[J].中草药,2016,47(8):1397-1400.
[10]李思明,冯怡,曾星.HPLC-APCI-MS/MS法同时测定猪苓颗粒中麦角甾酮与麦角甾醇的含量[J].药物分析杂志,2014,34(4):649-653.
[11]赵英永,程显隆,张萍,等.HPLC法测定猪苓中麦角甾酮的含量[J].药物分析杂志,2009,29(9):1579-1581.