药用真菌梨形马勃的生药学研究
|
摘要
目的对梨形马勃进行生药学研究,为其开发应用提供依据。方法采集梨形马勃子实体,进行基原鉴别、性状鉴别、显微鉴别、理化鉴别,结合文献资料确定其为梨形马勃Lycoperdon pyriforme Schaeff.。利用薄层色谱法建立梨形马勃专属性的薄层鉴别特征,利用高效液相色谱法测定其化学成分含量。结果薄层鉴别研究发现梨形马勃具有多个专属性的薄层色谱斑点,并含有麦角甾醇,麦角甾酮。高效液相色谱法测定其麦角甾醇含量为1.02mg/g、麦角甾酮含量为68.5μg/g。结论通过梨形马勃性状鉴别、显微鉴别、理化鉴别、薄层鉴别,可综合鉴别梨形马勃的真伪,为其鉴别提供了参考依据。麦角甾醇和麦角甾酮含量可作为其质量控制的重要指标,也为今后梨形马勃开发利用提供科学依据。
Objective The pharmacognostics of medicinal fungi Lycoperdon pyriforme Schaeff. were studied,which provided a identifying basis for the development and application. Methods The sporophore of Lycoperdon pyriforme was collected so that its species was identified by using original identification,characteristic identification,microscopic identification and the physical and chemical identification as well as literature. TLC was used to establish the specificity of Lycoperdon pyriforme,and its chemical composition content were determined by HPLC. Results A plurality of specific TLC spots were found in TLC study,including ergosterol and ergotsterone. The ergosterol content were determined of 1. 02 mg/g using HPLC methodand the content of ergotsterone was 68. 5μg/g in Lycoperdon pyriforme. Conclusion We can identify comprehensively the authenticity of Lycoperdon pyriforme by using literature textual criticism,original identification,characteristic identification,microscopic identification and the physical and chemical identification,it provided the referenced evidence for the identification of Lycoperdon pyriforme. The content of er gosterol and ergotsterone can be selected as important indicators for quality control in Lycoperdon pyriforme,and provided scientific datas for its development and utilization in the future.
Objective The pharmacognostics of medicinal fungi Lycoperdon pyriforme Schaeff. were studied,which provided a identifying basis for the development and application. Methods The sporophore of Lycoperdon pyriforme was collected so that its species was identified by using original identification,characteristic identification,microscopic identification and the physical and chemical identification as well as literature. TLC was used to establish the specificity of Lycoperdon pyriforme,and its chemical composition content were determined by HPLC. Results A plurality of specific TLC spots were found in TLC study,including ergosterol and ergotsterone. The ergosterol content were determined of 1. 02 mg/g using HPLC methodand the content of ergotsterone was 68. 5μg/g in Lycoperdon pyriforme. Conclusion We can identify comprehensively the authenticity of Lycoperdon pyriforme by using literature textual criticism,original identification,characteristic identification,microscopic identification and the physical and chemical identification,it provided the referenced evidence for the identification of Lycoperdon pyriforme. The content of er gosterol and ergotsterone can be selected as important indicators for quality control in Lycoperdon pyriforme,and provided scientific datas for its development and utilization in the future.
引文
[1]吴兴亮,卯晓岚,图力古尔,等.中国药用真菌[M].北京:科学出版社,2013:493.
[2]戴玉成,图力古尔,崔宝凯.中国药用真菌图志[M].哈尔滨:东北林业大学出版社,2013:330.
[3]国家药典委员会.中国药典,一部[S].北京:中国医药科技出版社,2015:50.
[4]韩冰雪.吉林省腹菌类物种多样性编目[D].吉林农业大学硕士学位论文,2016.
[5]宫诗林,曹玉峰,王淑敏.吉林省食药用真菌马勃的资源及鉴别研究[J].亚太传统医药,2013,9(12):49.
[6]曹龙辉,李晓珺,赵文红,等.麦角甾醇的研究进展[J].中国酿造,2014,33(4):9.
[7]Li A,Liu Y,Wang L,et al.Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice[J].Evid Based Complement Alternat Med,2015,2015:1.
[8]Zhang SY,Xu LT,Li AX,et al.Effects of Ergosterol,Isolated from Scleroderma Polyrhizum Pers.,on Lipopolysaccharide-Induced Inflammatory Responses in Acute Lung Injury[J].Inflam mation,2015,38(5):1979..
[9]陈晗,陈丹倩,李全福,等.麦角甾酮的药理活性、药代动力学及含量测定研究进展[J].中国中药杂志,2014,39(20):3905.
[10]赵英永.中药猪苓的化学成分及其药理学研究[D].西北大学博士学位论文,2010.
[2]戴玉成,图力古尔,崔宝凯.中国药用真菌图志[M].哈尔滨:东北林业大学出版社,2013:330.
[3]国家药典委员会.中国药典,一部[S].北京:中国医药科技出版社,2015:50.
[4]韩冰雪.吉林省腹菌类物种多样性编目[D].吉林农业大学硕士学位论文,2016.
[5]宫诗林,曹玉峰,王淑敏.吉林省食药用真菌马勃的资源及鉴别研究[J].亚太传统医药,2013,9(12):49.
[6]曹龙辉,李晓珺,赵文红,等.麦角甾醇的研究进展[J].中国酿造,2014,33(4):9.
[7]Li A,Liu Y,Wang L,et al.Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice[J].Evid Based Complement Alternat Med,2015,2015:1.
[8]Zhang SY,Xu LT,Li AX,et al.Effects of Ergosterol,Isolated from Scleroderma Polyrhizum Pers.,on Lipopolysaccharide-Induced Inflammatory Responses in Acute Lung Injury[J].Inflam mation,2015,38(5):1979..
[9]陈晗,陈丹倩,李全福,等.麦角甾酮的药理活性、药代动力学及含量测定研究进展[J].中国中药杂志,2014,39(20):3905.
[10]赵英永.中药猪苓的化学成分及其药理学研究[D].西北大学博士学位论文,2010.