CdSe/ZnS量子点的合成及对阿萨尔吉亚芽孢杆菌标记
|
摘要
目的:制备CdSe/ZnS核壳型量子点,并用量子点标记阿萨尔吉亚芽孢杆菌,为建立生防菌阿萨尔吉亚芽孢杆菌的荧光探针标记提供科学依据。方法:用化学方法合成CdSe量子点,再合成CdSe/ZnS核壳型量子点,并用化学偶联方法将量子点标记阿萨尔吉亚芽孢杆菌。结果:当Cd:Se摩尔比为2:1,反应时间为40 min,温度为30℃,pH为8. 0时,获得荧光性能较好的CdSe量子点,CdSe在与Na_2S和ZnSO_4 50℃反应1 h,得到CdSe/ZnS核壳型量子点。以MPA修饰的CdSe/ZnS核壳型量子点具有良好的荧光强度和分散性。加入EDC和NHS偶联剂的芽孢杆菌样本的标记效率明显高于不加偶联剂的样本。结论:CdSe/ZnS核壳型量子点的标记效率为24%。
Objective: CdSe/ZnS quantum dots were synthesized and used as fluorescence probes of Bacillus axarquiensis, so as to provide a scientific basis of the establishment of new technology for detection of antagonistic bacteria. Methods: Stable CdSe/ZnS quantum dots were synthesized by an aqueous phase route and the synthetic conditions were optimized, and then, Bacillus axarquiensis were labeled with quantum dots by chemical coupling method. Results: CdSe/ZnS quantum dots with good fluorescence properties were obtained at the following conditions: molar ratio Cd:Se was 2:1, pH was 8, the temperature was 30 ℃, the reaction time was 40 min. And then, CdSe/ZnS quantum dots were synthesized successfully after the reaction of CdSe with Na_2S and ZnSO_4 at 50 ℃ for 1 h. The CdSe/ZnS quantum dots modified by MPA have stable fluorescence intensity and good dispersibility. B. axarquiensis labeling efficiency was significantly higher incubation with EDC and NHS coupling agent. Conclusion:The CdSe/ZnS core-shell quantum dots labeling efficiency was 24%.
Objective: CdSe/ZnS quantum dots were synthesized and used as fluorescence probes of Bacillus axarquiensis, so as to provide a scientific basis of the establishment of new technology for detection of antagonistic bacteria. Methods: Stable CdSe/ZnS quantum dots were synthesized by an aqueous phase route and the synthetic conditions were optimized, and then, Bacillus axarquiensis were labeled with quantum dots by chemical coupling method. Results: CdSe/ZnS quantum dots with good fluorescence properties were obtained at the following conditions: molar ratio Cd:Se was 2:1, pH was 8, the temperature was 30 ℃, the reaction time was 40 min. And then, CdSe/ZnS quantum dots were synthesized successfully after the reaction of CdSe with Na_2S and ZnSO_4 at 50 ℃ for 1 h. The CdSe/ZnS quantum dots modified by MPA have stable fluorescence intensity and good dispersibility. B. axarquiensis labeling efficiency was significantly higher incubation with EDC and NHS coupling agent. Conclusion:The CdSe/ZnS core-shell quantum dots labeling efficiency was 24%.
引文
[1]Murray C B,Kagan C R,Bawendi M G.Synthesis and Characterization of Monodisperse Nanocrystals and ClosePacked Nanocrystal Assemblies[J].Annual Review of Materials Research,2000,30(1):545-610.
[2]Chen S T,Zhang Z Q,Zhang X L,et al.CdSe quantum dots decorated by mercaptosuccinic acid as fluorescence probe for Cu2+[J].Journal of Luminescence.2011,131(5):947-951.
[3]郑爱芳,陈金龙.CdTe纳米棒的水相合成与铜离子识别研究[J].无机材料学报.2009,24(02):251-254.
[4]俞英.3-巯基丙酸修饰的CdSe/ZnS量子点的合成及测定牛血清白蛋白的研究[J].分析科学学报.2009,25(1):59-62.
[5]蔡晓军,于郭,崔文国,等.核壳结构CdS/ZnS荧光量子点的制备与荧光性能研究[J].功能材料.2009,40(10):1643-1646.
[6]Wang L,Niu M,Wu Z.In situ growth of CdSe/CdS quantum dots inside and outside of MWCNTs[J].Current Applied Physics,2009,9(5):1112-1116.
[7]Shah BS,Clark PA,Moioli EK,et al.Labeling of mesenchymal stem cells by bioconjugated quantum dots[J].Methods in Molecular Biology,2007,680(10):61-64.
[8]Ma L,Wu S M,Huang J,et al.Fluorescence in situ hybridization(FISH)on maize metaphase chromosomes with quantum dot-labeled DNA conjugates[J].Chromosoma,2008,117(2):181-187.
[9]周娟,周海峰,周广军,等.CdSe/CdZnS核壳结构量子点的制备及对大肠杆菌O157的生物标记[J].解放军预防医学杂志,2015,33(5):487-489.
[10]Poirier I,Kuhn L,Demortière A,et al.Ability of the marine bacterium Pseudomonas fluorescens BA3SM1 to counteract the toxicity of CdSe nanoparticles[J].Journal of Proteomics,2016,148:213-227
[11]Yang C,Xie H,Li Q C,et al.Adherence and interaction of cationic quantum dots on bacterial surfaces[J].Journal of Colloid&Interface Science,2015,450:388-395.
[12]Carrillo-Carrión C,Simonet B M,Valcárcel M.Colistinfunctionalised CdSe/ZnS quantum dots as fluorescent probe for the rapid detection of Escherichia coli[J].Biosensors&Bioelectronics,2011,26(11):4368-4374.
[13]高艳丽,宗花,李延辉,等.CdSe量子点荧光增敏法测定甘草酸[J].分析测试学报.2011,30(11):1241-1245.
[14]宫晓群.硒化镉量子点的合成、改性及荧光编码微球的制备[D].天津:天津大学.2009
[15]Chan W C,Nie S.Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,281(5385):2016-2018.
[16]Kho R,Torres M,Mehra R,et al.A Simple Colloidal Synthesis for Gram-Quantity Production of Water-Soluble ZnSNanocrystal Powders[J].Journal of Colloid and Interface Science.2000,227(2):561-566.
[17]Xie H Y,Liang J G,Zhang Z L,et al.Luminescent CdSeZnS quantum dots as selective Cu2+probe[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy.2004,60(11):2527-2530.
[18]Ji X,Zheng J,Xu J,et al.CdSe/Zn S Quantum Dots and Organophosphorus Hydrolase Bioconjugate as Biosensors for Detection of Paraoxon[J].The Journal of Physical Chemistry B.2005,109(9):3793-3799.
[19]傅昕,张何,胡家义,等.基于CdSe/ZnS核壳型量子点的高灵敏、快速细菌计数新方法的研究[J].传感技术学报,2012,25(6):723-728.
[20]王晓暄,曲峰,陈祖彬,等.用量子点标记活细胞[J].生物物理学报,2010,26(5):365-372.
[21]刘晓红,罗金平,田青,等.荧光量子点免疫标记法检测炭疽芽孢杆菌[J].分析化学,2011,39(2):163-167.
[22]蔡朝霞,阮晓娟,石宝琴,等.水溶性CdSe量子点的合成及其作为荧光探针对大肠杆菌的快速检测[J].分析试验室,2011,30(3):107-110.
[2]Chen S T,Zhang Z Q,Zhang X L,et al.CdSe quantum dots decorated by mercaptosuccinic acid as fluorescence probe for Cu2+[J].Journal of Luminescence.2011,131(5):947-951.
[3]郑爱芳,陈金龙.CdTe纳米棒的水相合成与铜离子识别研究[J].无机材料学报.2009,24(02):251-254.
[4]俞英.3-巯基丙酸修饰的CdSe/ZnS量子点的合成及测定牛血清白蛋白的研究[J].分析科学学报.2009,25(1):59-62.
[5]蔡晓军,于郭,崔文国,等.核壳结构CdS/ZnS荧光量子点的制备与荧光性能研究[J].功能材料.2009,40(10):1643-1646.
[6]Wang L,Niu M,Wu Z.In situ growth of CdSe/CdS quantum dots inside and outside of MWCNTs[J].Current Applied Physics,2009,9(5):1112-1116.
[7]Shah BS,Clark PA,Moioli EK,et al.Labeling of mesenchymal stem cells by bioconjugated quantum dots[J].Methods in Molecular Biology,2007,680(10):61-64.
[8]Ma L,Wu S M,Huang J,et al.Fluorescence in situ hybridization(FISH)on maize metaphase chromosomes with quantum dot-labeled DNA conjugates[J].Chromosoma,2008,117(2):181-187.
[9]周娟,周海峰,周广军,等.CdSe/CdZnS核壳结构量子点的制备及对大肠杆菌O157的生物标记[J].解放军预防医学杂志,2015,33(5):487-489.
[10]Poirier I,Kuhn L,Demortière A,et al.Ability of the marine bacterium Pseudomonas fluorescens BA3SM1 to counteract the toxicity of CdSe nanoparticles[J].Journal of Proteomics,2016,148:213-227
[11]Yang C,Xie H,Li Q C,et al.Adherence and interaction of cationic quantum dots on bacterial surfaces[J].Journal of Colloid&Interface Science,2015,450:388-395.
[12]Carrillo-Carrión C,Simonet B M,Valcárcel M.Colistinfunctionalised CdSe/ZnS quantum dots as fluorescent probe for the rapid detection of Escherichia coli[J].Biosensors&Bioelectronics,2011,26(11):4368-4374.
[13]高艳丽,宗花,李延辉,等.CdSe量子点荧光增敏法测定甘草酸[J].分析测试学报.2011,30(11):1241-1245.
[14]宫晓群.硒化镉量子点的合成、改性及荧光编码微球的制备[D].天津:天津大学.2009
[15]Chan W C,Nie S.Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,281(5385):2016-2018.
[16]Kho R,Torres M,Mehra R,et al.A Simple Colloidal Synthesis for Gram-Quantity Production of Water-Soluble ZnSNanocrystal Powders[J].Journal of Colloid and Interface Science.2000,227(2):561-566.
[17]Xie H Y,Liang J G,Zhang Z L,et al.Luminescent CdSeZnS quantum dots as selective Cu2+probe[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy.2004,60(11):2527-2530.
[18]Ji X,Zheng J,Xu J,et al.CdSe/Zn S Quantum Dots and Organophosphorus Hydrolase Bioconjugate as Biosensors for Detection of Paraoxon[J].The Journal of Physical Chemistry B.2005,109(9):3793-3799.
[19]傅昕,张何,胡家义,等.基于CdSe/ZnS核壳型量子点的高灵敏、快速细菌计数新方法的研究[J].传感技术学报,2012,25(6):723-728.
[20]王晓暄,曲峰,陈祖彬,等.用量子点标记活细胞[J].生物物理学报,2010,26(5):365-372.
[21]刘晓红,罗金平,田青,等.荧光量子点免疫标记法检测炭疽芽孢杆菌[J].分析化学,2011,39(2):163-167.
[22]蔡朝霞,阮晓娟,石宝琴,等.水溶性CdSe量子点的合成及其作为荧光探针对大肠杆菌的快速检测[J].分析试验室,2011,30(3):107-110.