应用细菌回复突变试验研究贻贝中麻痹性贝毒的致突变性
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摘要
目的了解贻贝中麻痹性贝类毒素的致突变性。方法采用TA97、TA98、TAl00、TAl02测试菌株,在-S9和+S9条件下分别对0.0029～1.83μg STX/皿剂量范围内的贻贝中麻痹性贝类毒素进行检测。结果在2种不同的条件下,各剂量水平的4种测试菌株都能正常生长;所有剂量的回变菌落数与对照组相比均无1倍及以上的显著增加,且无剂量-反应关系。结论在该试验条件下,贻贝中麻痹性贝毒未见致突变性作用。
Objective The aim of this study was to assess the mutagenicity effects of paralytic shellfish poisoning in Mytilus edulis. Methods In the presence and or absence of S9 activator, the mutagenicity of PSP in Mytilus edulis at the concentrations from 0.0029-1.83 μg/petri dish were observed in the TA series of Salmonella typhimurium strains TA97,TA98, TAl00 and TAl02. Results All test materials did not affect the normal growth of the four-tested bacterium at all dosages with or without S9. Compared with the control group, all reversion mutations had no significant up-regulation. No dose-response relationship was found. Conclusion Under this experimental condition, PSP in Mytilus edulis had no mutagenicity in this test.
Objective The aim of this study was to assess the mutagenicity effects of paralytic shellfish poisoning in Mytilus edulis. Methods In the presence and or absence of S9 activator, the mutagenicity of PSP in Mytilus edulis at the concentrations from 0.0029-1.83 μg/petri dish were observed in the TA series of Salmonella typhimurium strains TA97,TA98, TAl00 and TAl02. Results All test materials did not affect the normal growth of the four-tested bacterium at all dosages with or without S9. Compared with the control group, all reversion mutations had no significant up-regulation. No dose-response relationship was found. Conclusion Under this experimental condition, PSP in Mytilus edulis had no mutagenicity in this test.
引文
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[5]国家卫生和计划生育委员会,国家食品药品监督管理总局.GB5009.213-2016食品安全国家标准贝类中麻痹性贝类毒素的测定[S].北京:中国标准出版社,2016.
[6]中华人民共和国国家卫生和计划生育委员会.GB 15193.4-2014食品安全国家标准细菌回复突变试验[S].北京:中国标准出版社,2016.
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[2]彭志兰,罗海军,王维洁,等.舟山海域麻痹性贝类毒素污染情况及其2种检测方法比较[J].食品安全质量检测学报,2017,8(4):1436-1440.
[3]孙烨,刘威,郑剑,等.2011-2014年深圳市贝类产品生物毒素污染状况调查[J].癌变·畸变·突变,2016,28(2):149-154.
[4]吴海燕,郭萌萌,谭志军,等.短裸甲藻毒素(Brevetoxin, BTX)研究进展[J].中国渔业质量与标准,2011,1(1):31-39.
[5]国家卫生和计划生育委员会,国家食品药品监督管理总局.GB5009.213-2016食品安全国家标准贝类中麻痹性贝类毒素的测定[S].北京:中国标准出版社,2016.
[6]中华人民共和国国家卫生和计划生育委员会.GB 15193.4-2014食品安全国家标准细菌回复突变试验[S].北京:中国标准出版社,2016.
[7]肖凯,李宏霞.遗传毒性试验方法应用现况与研究进展[J].现代预防医学,2004,31(4):524-526.
[8]李明远,徐志凯.医学微生物学[M].3版.北京:人民卫生出版社,2015:55-57.
[9]刘洁生,刘玉荣,聂利华,等.大鼠低剂量长期摄入麻痹性贝毒的毒性研究[J].中国病理生理杂志,2005,21(7):1368-1373.