复方苦参注射液影响放射后鼻咽上皮细胞存活及其机制的研究
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摘要
目的观察复方苦参注射液对放射后鼻咽上皮细胞(NP69)存活的影响,并探讨其放射防护作用机制。方法采用四甲基偶氮唑盐(MTT)法检测电离辐射后NP69细胞的增殖情况;采用流式细胞法观察复方苦参注射液对电离辐射后NP69细胞凋亡的影响;克隆形成试验检测各组细胞的克隆形成率;采用DCFH-DA荧光探针检测复方苦参注射液对电离辐射后NP69细胞氧自由基的清除作用。结果苦参干预组NP69细胞的增殖率高于单纯放射组(P<0.05);苦参干预组NP69细胞的凋亡率较单纯放射组明显减少(P<0.05);苦参干预组NP69细胞克隆形成率高于单纯放射组(P <0.05);经复方苦参注射液处理后,NP69细胞氧自由基活性较单纯放射组明显降低(P <0.05)。结论复方苦参注射液可能是通过清除NP69细胞中辐射产生的氧自由基,从而抑制辐射后NP69细胞凋亡并促进其增殖。
Objective To investigate the effect of compound Kushen injection on the survival of nasopharyngeal epithelial cells(NP69) after radiation, thus to explore its radioprotective mechanism. Methods Methylthiazolyl tetrazolium(MTT)assay was used to observe effect of compound Kushen injection on the proliferation of NP69 cells after radiation. Flow cytometry was used to detect apoptosis rate of NP69 cells. The clone formation rates of cells in various groups were detected by clone formation assay. DCFH-DA fluorescence probe was used to detect the level of oxygen radicals. Results After radiation,compared with the radiation alone group,the proliferation rate of NP69 cells in Kushen intervention group was higher(P<0.05), with lower apoptosis rate(P<0.05), higher clone formation rate(P<0.05), and decreased level of oxygen radicals(P<0.05). Conclusion Compound Kushen injection has effects on inhibiting the apoptosis and promoting the proliferation of NP69 cells after radiation by clearing oxygen radicals induced by radiation.
Objective To investigate the effect of compound Kushen injection on the survival of nasopharyngeal epithelial cells(NP69) after radiation, thus to explore its radioprotective mechanism. Methods Methylthiazolyl tetrazolium(MTT)assay was used to observe effect of compound Kushen injection on the proliferation of NP69 cells after radiation. Flow cytometry was used to detect apoptosis rate of NP69 cells. The clone formation rates of cells in various groups were detected by clone formation assay. DCFH-DA fluorescence probe was used to detect the level of oxygen radicals. Results After radiation,compared with the radiation alone group,the proliferation rate of NP69 cells in Kushen intervention group was higher(P<0.05), with lower apoptosis rate(P<0.05), higher clone formation rate(P<0.05), and decreased level of oxygen radicals(P<0.05). Conclusion Compound Kushen injection has effects on inhibiting the apoptosis and promoting the proliferation of NP69 cells after radiation by clearing oxygen radicals induced by radiation.
引文
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[2]MARIA OM,ELIOPOULOS N,MUANZA T.Radiation-induced oral mucositis[J].Front Oncol,2017,7:89-96.
[3]邓守恒,段霞,陈萍,等.复方苦参注射液联合调强放疗及腔内后装治疗宫颈癌疗效观察[J].现代中西医结合杂志,2015,24(20):2185-2187.
[4]宋海平,张宗春.复方苦参注射液联合放化疗治疗局部晚期鼻咽癌[J].中国肿瘤外科杂志,2014,6(5):279-281.
[5]黄奕雪,郭玉明,桑秀秀,等.复方苦参注射液治疗癌性疼痛的系统评价[J].中国实验方剂学杂志,2016,22(2):172-179.
[6]GUO Y,HUANG Y,SHEN H,et al.Efficacy of compound Kushen Injection in relieving cancer-related pain:a systematic review and meta-analysis[J].Evid Based Complement Alternat Med,2015(4):319-325.
[7]TROTTI A,BELLM LA,EPSTEIN JB,et al.Mucositis incidence,severity and associated outcomes in patients with head and neck cancer receiving radiotherapy with or without chemotherapy:a systematic literature review[J].Radiother Oncol,2003,66(3):253-262.
[8]NONZEE NJ,DANDADE NA,PATEL U,et al.Evaluating the supportive care costs of severe radiochemotherapy-induced mucositis and pharyngitis:Results from a Northwestern University Costs of Cancer Program pilot study with head and neck and nonsmall cell lung cancer patients who received care at a county hospital,a Veterans Administration hospital,or a comprehensive cancer care center[J].Cancer,2008,113(6):1446-1452.
[9]RUBENSTEIN E B,PETERSON D E,SCHUBERT M,et al.Clinical practice guidelines for the prevention and treatment of cancer therapy-induced oral and gastrointestinal mucositis[J].Cancer,2004,100(9 Suppl):2026-2046.
[10]KEEFE D M,SCHUBERT M M,ELTING L S,et al.Updated clinical practice guidelines for the prevention and treatment of mucositis[J].Cancer,2007,109(5):820-831.
[11]VERA-LLONCH M,OSTER G,HAGIWARA M,et al.Oral mucositis in patients undergoing radiation treatment for head and neck carcinoma[J].Cancer,2006,106(2):329-336.
[12]陈谦明.口腔黏膜病学[M].3版.北京:人民卫生出版社,2008:77-78.
[13]DTIRR H,MEINEKE V.Acute radiation syndrome caused by accidental radiation exposure-therapeutic principles[J].BMC Med,2011,12(9):126-128.
[14]DURACKOVA Z.Some current insights into oxidative stress[J].Physiol Res,2010,59(4):459-469.
[15]ARORA R,GUPTA D,SHARMA A K,et al.Modification of radiation induced damage in mammalian modern systems by natural plant products:implications for radiation protection in proceedings of the IUPAC[J].International Conference on Biodiversity and Natural Products,2004,33(4):56-63.
[16]田晓华,丛建波,孙存善,等.氧化苦参碱清除·OH自由基作用及对胸腺嘧啶核苷酸辐射防护作用的ESR研究[J].解放军预防医学杂志,1996,14(6):412-414.