健骨颗粒乙酸乙酯萃取部位对卵巢切除大鼠骨质量的影响
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摘要
目的:研究健骨颗粒乙酸乙酯萃取部位对卵巢切除大鼠骨质量的影响。方法:采用切除雌性SD大鼠双侧卵巢建立绝经后骨质疏松症模型。运用双能X线4500W骨密度仪测定骨密度,万能材料试验机检测骨组织的最大载荷。骨组织石蜡包埋切片后行Masson染色,观察骨组织形态,并做骨小梁面积百分比计量。通过酶联免疫吸附法检测血清中的骨碱性磷酸酶(BALP)、雌二醇(E2)、抗酒石酸酸性磷酸酶(TRAP)的含量。结果:与空白组比较,乙酸乙酯组术后4、12周BMD显著升高(P<0.01),术后12周胫骨三点抗弯最大载荷显著升高(P<0.01),术后8、12周腰椎、胫骨近干骺端骨小梁面积百分比及血清E2水平显著升高(P<0.01),术后12周血清BALP水平显著升高(P<0.01),术后8周血清TRAP水平显著下降(P<0.05)。结论:健骨颗粒乙酸乙酯萃取部位能有效的改善卵巢切除术后大鼠机体的骨代谢水平,改善骨质量。
Objective: To observe the effects of ethyl acetate extraction of Jiangu Granule on the bone quality of ovariectimized rats. Methods: The postmenopausal osteoporosis rat model was established by removing bilateral ovaries of SD female rats. The bone density of bone tissue and biomechanics were detected. The morphology of the bone was observed by Masson staining. The concentration of E2, TRAP, and BALP in the serum was detected by the method of ELISA. Results: Compared with the blank group, the BMD at 4 and 12 weeks after surgery increased significantly(P<0.01), the maximum load of the tibia at 12 weeks after surgery increased significantly(P<0.01), the area percentage of bone trabeculae and E2 at 8 and 12 weeks after surgery increased significantly(P<0.01), the serum BALP level at 12 weeks after surgery increased significantly(P<0.01), the serum BALP level at 12 weeks after surgery increased significantly(P<0.01), the serum TRAP level at 8 weeks after surgery decreased significantly(P<0.05). Conclusion: The bone metabolism and bone quality of ovariectimized rats were effectively improved in ethyl acetate group.
Objective: To observe the effects of ethyl acetate extraction of Jiangu Granule on the bone quality of ovariectimized rats. Methods: The postmenopausal osteoporosis rat model was established by removing bilateral ovaries of SD female rats. The bone density of bone tissue and biomechanics were detected. The morphology of the bone was observed by Masson staining. The concentration of E2, TRAP, and BALP in the serum was detected by the method of ELISA. Results: Compared with the blank group, the BMD at 4 and 12 weeks after surgery increased significantly(P<0.01), the maximum load of the tibia at 12 weeks after surgery increased significantly(P<0.01), the area percentage of bone trabeculae and E2 at 8 and 12 weeks after surgery increased significantly(P<0.01), the serum BALP level at 12 weeks after surgery increased significantly(P<0.01), the serum BALP level at 12 weeks after surgery increased significantly(P<0.01), the serum TRAP level at 8 weeks after surgery decreased significantly(P<0.05). Conclusion: The bone metabolism and bone quality of ovariectimized rats were effectively improved in ethyl acetate group.
引文
[1]林燕萍,马建华,冯尔宥,等.健骨颗粒对去卵巢大鼠骨质疏松症预防作用的研究.中国中西医结合杂志,2002,22(5):369-371
[2]林燕萍,周瑞祥,郭世明,等.健骨颗粒对去卵巢骨质疏松模型大鼠骨质量的影响.中国中西医结合杂志,2004,24(5):431-434
[3]林煜,卢天祥,吴银生,等.健骨颗粒含药血清培养成骨细胞整合素/黏着斑激酶信号通路相关因子的表达.中国组织工程研究与临床康复,2010,14(46):8625-8630
[4]贾晓康,黄云梅,李超雄,等.健骨颗粒通过G1/S期调控蛋白对成骨细胞增殖的影响.中国医药导报,2016,13(9):9-13
[5]张媛媛,何嘉承,林煜,等.健骨颗粒含药血清对体外破骨细胞CAⅡ、CK、MMP-9 mRNA表达的影响.中国骨质疏松杂志,2015,21(12):1436-1440
[6]林海鸣,林燕萍,吴银生.健骨颗粒含药血清对成骨样细胞增殖及PCNA、Cyclin D1 mRNA表达的影响.江西中医学院学报,2012,24(2):26-29
[7]林海鸣,林燕萍,吴银生.健骨颗粒含药血清对成骨样细胞OPG、RANKL mRNA表达的影响.福建中医药大学学报,2012,22(1):19-21
[8]林煜,吴银生,卢天祥,等.健骨颗粒对成骨细胞分化的影响.中华中医药杂志,2012,27(1):165-168
[9]林煜,张怡元,黄云梅,等.健骨颗粒对大鼠骨组织端粒酶逆转录酶增龄性变化的干预作用.中华中医药杂志,2016,31(12):5333-5338
[10]周力学.绝经后骨质疏松症.新医学,2007,38(9):611-613
[11]彭丽红,马改霞.骨质疏松症的中医药治疗研究进展.西北药学杂志,2013,28(1):98-101
[12]张鑫,肖鲁伟,童培建.补肾活血汤防治绝经后骨质疏松症的实验研究.中医正骨,2010,22(2):3-6
[13]林煜,张怡元,吴银生,等.肾主骨理论与雌激素介导的骨组织端粒酶逆转录酶增龄性变化之关系研究.中华中医药杂志,2015,30(4):1066-1070
[14]覃裕,邱冰,朱思刚,等.唑来膦酸与阿仑膦酸钠治疗骨质疏松患者的疗效比较及对血清25-(OH)D、BALP、BGP的影响分析.中国骨质疏松杂志,2016,22(8):1035-1038
[2]林燕萍,周瑞祥,郭世明,等.健骨颗粒对去卵巢骨质疏松模型大鼠骨质量的影响.中国中西医结合杂志,2004,24(5):431-434
[3]林煜,卢天祥,吴银生,等.健骨颗粒含药血清培养成骨细胞整合素/黏着斑激酶信号通路相关因子的表达.中国组织工程研究与临床康复,2010,14(46):8625-8630
[4]贾晓康,黄云梅,李超雄,等.健骨颗粒通过G1/S期调控蛋白对成骨细胞增殖的影响.中国医药导报,2016,13(9):9-13
[5]张媛媛,何嘉承,林煜,等.健骨颗粒含药血清对体外破骨细胞CAⅡ、CK、MMP-9 mRNA表达的影响.中国骨质疏松杂志,2015,21(12):1436-1440
[6]林海鸣,林燕萍,吴银生.健骨颗粒含药血清对成骨样细胞增殖及PCNA、Cyclin D1 mRNA表达的影响.江西中医学院学报,2012,24(2):26-29
[7]林海鸣,林燕萍,吴银生.健骨颗粒含药血清对成骨样细胞OPG、RANKL mRNA表达的影响.福建中医药大学学报,2012,22(1):19-21
[8]林煜,吴银生,卢天祥,等.健骨颗粒对成骨细胞分化的影响.中华中医药杂志,2012,27(1):165-168
[9]林煜,张怡元,黄云梅,等.健骨颗粒对大鼠骨组织端粒酶逆转录酶增龄性变化的干预作用.中华中医药杂志,2016,31(12):5333-5338
[10]周力学.绝经后骨质疏松症.新医学,2007,38(9):611-613
[11]彭丽红,马改霞.骨质疏松症的中医药治疗研究进展.西北药学杂志,2013,28(1):98-101
[12]张鑫,肖鲁伟,童培建.补肾活血汤防治绝经后骨质疏松症的实验研究.中医正骨,2010,22(2):3-6
[13]林煜,张怡元,吴银生,等.肾主骨理论与雌激素介导的骨组织端粒酶逆转录酶增龄性变化之关系研究.中华中医药杂志,2015,30(4):1066-1070
[14]覃裕,邱冰,朱思刚,等.唑来膦酸与阿仑膦酸钠治疗骨质疏松患者的疗效比较及对血清25-(OH)D、BALP、BGP的影响分析.中国骨质疏松杂志,2016,22(8):1035-1038