甘蔗叶水解生产木糖工艺研究
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摘要
为解决当前甘蔗叶生产木糖的高额操作成本或处理操作工艺及路线限制,以甘蔗叶为材料,进行了高温蒸煮法预处理甘蔗叶、硫酸催化水解高温蒸煮后的甘蔗叶、甘蔗叶水解液分离提纯等工艺研究。结果表明,最佳高温蒸煮工艺条件为:甘蔗叶粒径为60目、蒸煮温度118℃、固液比1∶10、蒸煮1h;最佳水解条件为:硫酸浓度1.2%、固液比1∶8、水解时间2h、水解温度123℃;水解液经脱色、浓缩、发酵、离交、蒸发、结晶、离心干燥等工序得到纯度为99.967%的木糖产品。研究结果能为有效解决大量制糖工业剩余物的利用问题提供思路,为寻找一条甘蔗叶生产木糖的高效、环保、成本低、易于实现的工艺提供技术基础。
Using sugarcane leaf as material to solve the high running cost or treatment process and route restriction of xylose production. The way of treating sugarcane leaves with high temperature, acid hydrolysate of sugarcane leaves and the purification of acid hydrolysate liquor were mainly studied in the paper. The results indicated that the sugarcane leaves were best steamed at 118℃ for 1 h, the granularity was 60 mu,and the ratio of sugarcane leaves(g) to water volume(m L) was 1:10(w/v). The optimum hydrolysis conditions were as follows : the concentration of H2 SO4 was 1.2%, the temperature was 123℃ for 2 h, the ratio of sugarcane leaves(g) to H2 SO4 solution volume(m L) was 1:8(w/v). The xylose with purity of 99.967% was obtained after hydrolysate was treated by the following processes: decolorizing, concentrating, fermenting, disconnecting, ion exchange, evaporating,crystallizing, centrifuge separation and drying. The research results provided some thoughts for the useage of the massive residues from sugar industries and provided technological foundations for the high efficiency,environmental protection, low cost and easy to realize production process of xylose from sugarcane leaf.
Using sugarcane leaf as material to solve the high running cost or treatment process and route restriction of xylose production. The way of treating sugarcane leaves with high temperature, acid hydrolysate of sugarcane leaves and the purification of acid hydrolysate liquor were mainly studied in the paper. The results indicated that the sugarcane leaves were best steamed at 118℃ for 1 h, the granularity was 60 mu,and the ratio of sugarcane leaves(g) to water volume(m L) was 1:10(w/v). The optimum hydrolysis conditions were as follows : the concentration of H2 SO4 was 1.2%, the temperature was 123℃ for 2 h, the ratio of sugarcane leaves(g) to H2 SO4 solution volume(m L) was 1:8(w/v). The xylose with purity of 99.967% was obtained after hydrolysate was treated by the following processes: decolorizing, concentrating, fermenting, disconnecting, ion exchange, evaporating,crystallizing, centrifuge separation and drying. The research results provided some thoughts for the useage of the massive residues from sugar industries and provided technological foundations for the high efficiency,environmental protection, low cost and easy to realize production process of xylose from sugarcane leaf.
引文
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[15]周娟,黄伟红,杜锐锋,等.高压液相色谱对木糖母液的成分分析[J].中国食品添加剂,2011(1):239-226.
[16]张宇,许敬亮,袁振宏,等.甘蔗渣的不同预处理方法比较及其酶水解的类分形动力学[J].新能源进展,2013,1(2):166-169.
[17]玉米秸秆的不同预处理及其糖化发酵研究[D].济南:山东大学,2015.
[18]景文祥,杨国恩,韩莹莉,等.甘蔗渣中木聚糖的提取研究[J].中国食品添加剂,2013(2):88-93.
[19]童乃姗.蔗渣原料预处理对酶解糖化的影响[D].广州:华南农业大学,2016.
[20]肖代俊,杨登峰,黄志民,等.硫酸催化蔗髓半纤维素的水解条件研究[J].广西科学,2008,15(2):181-183.
[21]韦巧艳,李坚斌,崔素芬,等.木糖提取工艺的优化及其机理研究[J].轻工科技,2015(8):26-29.
[22]蒋琦霞.爆破秸秆酶法制备低聚木糖及其精制工艺研究[D].无锡:江南大学,2007.
[23]任鸿钧.木糖醇生产技术问答[M].北京:中国轻工业出版社,2008:110-11,125-126.
[24]曹如燕,杨严俊,徐雅琴.酵母发酵法脱除蛋清中葡萄糖的研究[J].四川食品与发酵,2005,41(1):23-26.
[25]杨丹.甘蔗渣生物炼制生产木糖的研究[D].济南:齐鲁工业大学,2014.
[26]张荣立.以木糖母液为原料的半乳糖醇生产工艺研究[D].济南:齐鲁工业大学,2012.
[2]S Boochapun,W Lamamorphanth,K Kamwilaisak.The Acid Hydrolysis of Sugarcane Leaves as a Biofeedstook for Bioethanol Production[J].Advanced Materials Research,2014,931-932:194-199.
[3]P Moodley,EBG Kana.Optimization of xylose and glucose production from sugarcane leaves(Saccharum officinarum)using hybrid pretreatment techniques and assessment for hydrogen generation at semi-pilot scale[J].International Journal of Hydrogen Energy,2015,40(10):3859-3867.
[4]P Moodley,EBG Kana.Comparative study of three optimized acid-based pretreatments for sugar recovery from sugarcane leaf waste:A sustainable feedstock for biohydrogen production[J].Engineering Science&Technology An International Journal,2017.https://doi.org/10.1016/j.jestch.2017.11.010.
[5]雷光鸿,崔素芬,柳嘉,刘健,李敏,李鲜能.甘蔗叶蒸汽爆破法提取木糖的工艺研究[J].广西轻工业,2011,27(4):25-27.
[6]雷光鸿,崔素芬,柳嘉,刘健,李敏,李鲜能.甘蔗叶蒸气爆破法提取木糖的工艺研究[J].食品科技,2009,34(8):146-150.
[7]雷光鸿,崔素芬,李辰,杨勇,李坚斌.酸催化水解处理甘蔗叶提取木糖的研究[J].食品科技,2015,40(5):220-223.
[8]雷光鸿,崔素芬,李辰,杨勇,李坚斌.甘蔗叶水解液提取木糖纯化工艺的研究[J].食品工业,2015,36(10):67-70.
[9]周开法.利用甘蔗叶生产木糖初探[J].企业科技与发展,2014(21):27-29.
[10]崔素芬.甘蔗叶资源化制备木糖的关键技术研究[D].南宁:广西大学,2009.
[11]崔素芬,李坚斌,杭方学,等.蒸汽爆碎处理甘蔗叶提取木糖的研究[J].食品工业科技,2010,31(1):217-219.
[12]蒙丽霞,梁欣泉,李敏,等.高温蒸煮预处理甘蔗叶制备木聚糖[J].食品与发酵工业,2012,38(1):105-108.
[13]李敏,李坚斌,梁欣泉,等.甘蔗叶酶法制取低聚木糖的工艺研究[J].中国酿造,2011,30(12):54-57.
[14]崔素芬.甘蔗叶资源化制备木糖的关键技术研究[D].南宁:广西大学,2009.
[15]周娟,黄伟红,杜锐锋,等.高压液相色谱对木糖母液的成分分析[J].中国食品添加剂,2011(1):239-226.
[16]张宇,许敬亮,袁振宏,等.甘蔗渣的不同预处理方法比较及其酶水解的类分形动力学[J].新能源进展,2013,1(2):166-169.
[17]玉米秸秆的不同预处理及其糖化发酵研究[D].济南:山东大学,2015.
[18]景文祥,杨国恩,韩莹莉,等.甘蔗渣中木聚糖的提取研究[J].中国食品添加剂,2013(2):88-93.
[19]童乃姗.蔗渣原料预处理对酶解糖化的影响[D].广州:华南农业大学,2016.
[20]肖代俊,杨登峰,黄志民,等.硫酸催化蔗髓半纤维素的水解条件研究[J].广西科学,2008,15(2):181-183.
[21]韦巧艳,李坚斌,崔素芬,等.木糖提取工艺的优化及其机理研究[J].轻工科技,2015(8):26-29.
[22]蒋琦霞.爆破秸秆酶法制备低聚木糖及其精制工艺研究[D].无锡:江南大学,2007.
[23]任鸿钧.木糖醇生产技术问答[M].北京:中国轻工业出版社,2008:110-11,125-126.
[24]曹如燕,杨严俊,徐雅琴.酵母发酵法脱除蛋清中葡萄糖的研究[J].四川食品与发酵,2005,41(1):23-26.
[25]杨丹.甘蔗渣生物炼制生产木糖的研究[D].济南:齐鲁工业大学,2014.
[26]张荣立.以木糖母液为原料的半乳糖醇生产工艺研究[D].济南:齐鲁工业大学,2012.