猪流行性腹泻病毒流行株M基因的克隆与序列分析
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摘要
采用RT-PCR方法对收集的2株猪流行性腹泻病毒(PEDV)临床毒株(D1、D3)的M基因进行克隆,并对其核苷酸和推导的氨基酸序列进行分析。结果表明:2株PEDV流行毒株M基因间核苷酸序列同源性为98.1%,氨基酸序列同源性为97.8%。流行毒株D1与参考株EAS1、SM98、CHM2013分离株亲缘性较近,流行毒株D3与参考株PEDV-LY、PEDV-WS、CH22分离株亲缘性较近。与参考株CV777相比,D1株M蛋白有4个抗原决定簇的位置和序列组成发生了变化。
The M genes of 2 clinical isolates of porcine epidemic diarrhea virus(PEDV),D1 and D3 were cloned by RT-PCR,then their nucleotide and encoding amino acid sequences were analyzed. The results showed that the nucleotide sequence homology of the 2 isolates was 98. 1 % and the homology of amino acid sequence was97. 8%. The phylogenetic relationship of the isolate D1 and the reference strain EAS1,SM98,CHM2013 isolates were relatively close, while the phylogenetic relationship of the isolate D3 and PEDV-LY, PEDV-WS, CH22 isolates were relatively close. Compared with the reference strain CV777,the location and sequence composition of 4 antigen determinants in M protein of isolate D1 were changed.
The M genes of 2 clinical isolates of porcine epidemic diarrhea virus(PEDV),D1 and D3 were cloned by RT-PCR,then their nucleotide and encoding amino acid sequences were analyzed. The results showed that the nucleotide sequence homology of the 2 isolates was 98. 1 % and the homology of amino acid sequence was97. 8%. The phylogenetic relationship of the isolate D1 and the reference strain EAS1,SM98,CHM2013 isolates were relatively close, while the phylogenetic relationship of the isolate D3 and PEDV-LY, PEDV-WS, CH22 isolates were relatively close. Compared with the reference strain CV777,the location and sequence composition of 4 antigen determinants in M protein of isolate D1 were changed.
引文
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[6]朱迪国,宋建德,袁丽萍,等.2013-2014年全球猪流行性腹泻重大疫情分析[J].中国动物检疫,2014,31(10):42-46.
[7]张志,董雅琴,刘爽,等.我国部分省份猪流行性腹泻的流行病学监测[J].中国动物检疫,2014,31(10):47-51.
[8]吴玉璐,虞凌雪,程群,等.猪流行性腹泻病毒M基因的表达及鉴定[J].中国动物传染病学报,2012,20(6):6-10.
[9]卢冰霞,秦毅斌,何颖,等.2011-2014年广西猪流行性腹泻病毒检测及其M基因的序列分析[J].中国畜牧兽医,2015,42(3):549-557.
[10]黄冬妮,黄良宗,马春全,等.广东省猪流行性腹泻病毒ORF3和M基因的克隆与序列分析[J].黑龙江畜牧兽医,2015(5):136-139.
[2]WANG E,GUO D,LI C,et al.Molecular Characterization of the ORF3 and S1 Genes of Porcine Epidemic Diarrhea Virus Non S-INDEL Strains in Seven Regions of China,2015[J].PLoS ONE,2016,11(8):e0160561.
[3]SU Y,LIU Y,CHEN Y,et al.Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene[J].Virol J,2016,13(1):192.
[4]LI Z L,ZHU L,MA J Y,et al.Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus(PEDV)field strains in south China[J].Virus Genes,2012,45(1):181-185.
[5]CHANG S H,BAE J L,KANG T J,et al.Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus[J].Mol Cells,2002,14(2):295-299.
[6]朱迪国,宋建德,袁丽萍,等.2013-2014年全球猪流行性腹泻重大疫情分析[J].中国动物检疫,2014,31(10):42-46.
[7]张志,董雅琴,刘爽,等.我国部分省份猪流行性腹泻的流行病学监测[J].中国动物检疫,2014,31(10):47-51.
[8]吴玉璐,虞凌雪,程群,等.猪流行性腹泻病毒M基因的表达及鉴定[J].中国动物传染病学报,2012,20(6):6-10.
[9]卢冰霞,秦毅斌,何颖,等.2011-2014年广西猪流行性腹泻病毒检测及其M基因的序列分析[J].中国畜牧兽医,2015,42(3):549-557.
[10]黄冬妮,黄良宗,马春全,等.广东省猪流行性腹泻病毒ORF3和M基因的克隆与序列分析[J].黑龙江畜牧兽医,2015(5):136-139.