火焰原子吸收法检测兔脑缺血再灌注损伤后血钙含量
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摘要
利用火焰原子吸收法对家兔脑缺血再灌注损伤后血钙的含量进行检测,并与假手术组进行比较,结果显示缺血再灌注损伤组血液中的钙含量高于假手术组(P<0.01),差异有统计学意义,并且缺血再灌注2h组和缺血再灌注4h组中差异也有明显统计学意义(P<0.05),本实验提示家兔发生局部脑缺血再灌注损伤后血液中的钙离子较正常状态下升高,并可通过火焰原子吸收法有效地测定血液中钙的含量。
We determined the blood calcium of rabbits with cerebral ischemia-reperfusion injury by flame atomic absorption spectrophotometry,and compared with sham operated controls,the results indicated that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased obviously(P<0.01).It have disparity between the two of cerebral ischemia-reperfusion injury groups(P<0.05).Our experiments displayed that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased,and we could make use of flame atomic absorption spectrophotometry method to determine calcium content in blood.
We determined the blood calcium of rabbits with cerebral ischemia-reperfusion injury by flame atomic absorption spectrophotometry,and compared with sham operated controls,the results indicated that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased obviously(P<0.01).It have disparity between the two of cerebral ischemia-reperfusion injury groups(P<0.05).Our experiments displayed that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased,and we could make use of flame atomic absorption spectrophotometry method to determine calcium content in blood.
引文
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[5]王红梅,张剑龙,腾清蕾,等.大鼠肝缺血再灌注损伤诱导型一氧化氮和酶表达与肝细胞凋亡的关系[J].新乡医学院学报,2006,23(1):42-44.
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[7]唐瑜,罗荣敬,周乐全,等.脑缺血再灌注损伤对大鼠海马神经元L-型钙通道的影响[J].中国病理生理杂志,2008,24(5):1030-1031,1034.
[8]谭婷,陈鹏,王美纳,等.脑缺血再灌注损伤机制研究进展[J].西北药学杂志,2005,20(3):144-146.
[9]Skulachev VP.Mitochondrial physiology and pathology;concepts of programmed death of organelles,cells andorganisms[J].Mol Aspects Med,1999,20(3):139-184.
[10]Tran QK,Ohashi K,Watanabe H.Calcium signalling inendothelia Cells[J].Cardiovasc Res,2000,48(1):13-22.
[11]Ding XS,Feng MJ.Pathophysiology and apoptosis inilschemic stroke[J].Int J Cerebrovasc Dis,2006,14:5-10.
[12]陈敏,黄勤烽.多通道火焰原子吸收光谱法测定全血与血浆中5种元素含量的相关性分析[J].分析仪器,2010,1:80-83.
[2]王毅刚,王允,孙晓晶,等.大鼠线栓法局灶性脑缺血再灌注损伤模型改进的实验研究[J].徐州医学院学报,2009,29(9):568-570.
[3]Pulsinelli WA,Buchan AM.The four vessel occlusion ratmodel;method for complete occlusion cerebral arteries andcontrol of collateral circulation[J].Stroke,1988,19(7):913-914.
[4]Bengisun J,Kksoy C,Bengisun JS,et al.Ischemia andreperfusion injury:prevention of pulmonary hypertension andleukosequestration following lower limb ischemia[J].ProstagLandins Leukot Essent Fatty Acids,1997,56(2):117-120.
[5]王红梅,张剑龙,腾清蕾,等.大鼠肝缺血再灌注损伤诱导型一氧化氮和酶表达与肝细胞凋亡的关系[J].新乡医学院学报,2006,23(1):42-44.
[6]焦勇刚,孙银平.参麦注射液对家兔缺血再灌注损伤肠微循环的保护作用[J].新乡医学院学报,2005,22(3):213-215.
[7]唐瑜,罗荣敬,周乐全,等.脑缺血再灌注损伤对大鼠海马神经元L-型钙通道的影响[J].中国病理生理杂志,2008,24(5):1030-1031,1034.
[8]谭婷,陈鹏,王美纳,等.脑缺血再灌注损伤机制研究进展[J].西北药学杂志,2005,20(3):144-146.
[9]Skulachev VP.Mitochondrial physiology and pathology;concepts of programmed death of organelles,cells andorganisms[J].Mol Aspects Med,1999,20(3):139-184.
[10]Tran QK,Ohashi K,Watanabe H.Calcium signalling inendothelia Cells[J].Cardiovasc Res,2000,48(1):13-22.
[11]Ding XS,Feng MJ.Pathophysiology and apoptosis inilschemic stroke[J].Int J Cerebrovasc Dis,2006,14:5-10.
[12]陈敏,黄勤烽.多通道火焰原子吸收光谱法测定全血与血浆中5种元素含量的相关性分析[J].分析仪器,2010,1:80-83.