摘要
以‘甜查理’草莓为试材,利用硫化氢(H_2S)供体硫氢化钠(NaHS)处理果实,以水为对照,研究在货架(20℃)及低温(2℃)贮藏过程中花青素、丙二醛(MDA)、总酚(TP)、氧化自由基吸收能力(ORAC)、DPPH自由基清除力、铁离子还原能力(FRAP)以及ABTS自由基清除能力变化。结果表明,与对照组相比,在20及2℃贮藏条件下,NaHS可延缓果实中花青素和MDA积累。在20℃贮藏过程中,NaHS虽然提高了果实内TP含量,但在2℃贮藏过程中,NaHS对TP影响不明显。在20℃贮藏条件下,与对照和0.4 mmol/L NaHS处理组相比,0.8、1.6和3.2 mmol/L NaHS处理组果实具有较高的ORAC氧化自由基吸收力、DPPH自由基清除力、FRAP铁离子还原能力以及ABTS自由基清除能力。在2℃贮藏条件下,NaHS对果实内ABTS自由基清除能力影响不明显,但1.6和3.2 mmol/L NaHS处理组果实具有较高的ORAC氧化自由基吸收力、DPPH自由基清除力和FRAP铁离子还原能力。采用1.6~3.2 mmol/L NaHS处理草莓果实有利于保持货架及低温下草莓果实内抗氧化活性稳定性。
The aim of this study was to investigate the changes of anthocyanidins content,malondialdehyde(MDA)content,total polyphenol content,oxygen radical absorption capacity(ORAC),2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging capacity,ferric reducing antioxidant power(FRAP),and 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS)radical scavenging capacity in strawberry fruit during shelf(20 ℃)and low temperature(2 ℃)storage after fumigating with sodium hydrosulfide solution(NaHS,the donor of H_2S). Results showed that,regardless of storage temperature,NaHS could delay the accumulation of anthocyanidins and MDA compared with control fruit. NaHS made TP content increase during storage at 20 ℃,while did not affect TP content when strawberry fruit were subjected to 2 ℃. During storage at 20 ℃,0.8. 1.6 and 3.2 mmol/L NaHS-treated fruit maintained higher levels of ORAC,DPPH radical scavenging capacity,FRAP,and ABTS radical scavenging capacity than control and 0.4 mmol/L NaHS-treated fruit. When storage temperature increase from 20 ℃ to 2 ℃,ABTS radical scavenging capacity did not affect by NaHS,but 1.6 and 3.2 mmol/L NaHS-treated fruit maintained higher levels of ORAC,DPPH radical scavenging capacity and FRAP than other treatments. NaHS concentration between 1.6 to 3.2 mmol/L had a great benefit for stabilizing antioxidant capacity during shelf or low temperature storage.
引文
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