坦索罗辛通过调控miR-141表达改善大鼠前列腺炎
摘要
目的探讨坦索罗辛通过miRNA改善大鼠前列腺炎的作用。方法利用角叉菜胶溶液诱导大鼠前列腺病变,建立前列腺炎小鼠模型后,HE染色及天狼星红染色观察前列腺组织病理形态。应用Real-time PCR技术检测坦索罗辛对大鼠炎症模型及大鼠前列腺上皮细胞炎症模型中miR-141表达影响;应用Western blot检测坦索罗辛对大鼠前列腺组织及前列腺上皮细胞中TGF-β_1和α-SMA蛋白表达影响;应用试剂盒检测坦索罗辛对前列腺组织及前列腺上皮细胞中炎症因子TNF-α表达水平影响。结果坦索罗辛能抑制miR-141在大鼠前列腺组织中的下调,同时抑制前列腺的弥漫性炎症、胶原聚集、TNF-α,TGF-β_1及α-SMA的表达;给予人前列腺细胞炎症模型坦索罗辛治疗并敲减miR-141,可逆转坦索罗辛的治疗作用。结论坦索罗辛能通过调控miR-141表达改善前列腺炎,为坦索罗辛临床应用提供理论基础。
引文
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[3]魏巍,候立朝,樊海宁,等.MicroRNA在肿瘤疾病中的应用研究[J].医药前沿,2017,09:93.
[4]王慧琦,高成金.miRNA与常见肺疾病的关系研究[J].同济大学学报,2014,06:27.
[5]Wentao Liu,Lin Qi.miRNA-141 and miRNA-200b are closely related to invasive ability and considered as decision-making biomarkers for the extent of PLND during cystectomy[J].BMC Cancer,2015,15:92.
[6]Igor Koturbash,William H Tolleson.MicroRNAs as pharmacogenomic biomarkers for drug efficacy and drug safety assessment[J].Biomark Med.Author manuscript.Biomark Med,2015,9(11):1153-1176.
[7]杨涛,吴柏霖,周辉,等.沙芭特在非细菌性前列腺炎症中的抗炎、抗纤维化及促进上皮细胞凋亡作用的研究[J].现代泌尿生殖肿瘤杂志,2017,(04).
[8]Wang L L,Huang Y H,Yan C Y,et al.N-acetylcysteine Ameliorates Prostatitis via miR-141 Regulating Keap1/Nrf2 Signaling[J].Inflammation.2016,39(2):1-10.
[9]Huang S,Wa Q,Pan J,et al.Downregulation of miR-141-3p promotes bone metastasis via activating NF-κB signaling in prostate cancer[J].Exp Clin Cancer Res.2017.36(1):173.
[10]Shen W S,Xu X Q,Zhai N N,et al.Potential mechanisms of microRNA-141-3p to alleviate chronic inflammatory pain by downregulation of downstream target gene HMGB1:in vitro and in vivo studies[J].Gene Therapy.2017,24(6):353-360.