种子内生细菌对黄华占水稻根系生长的影响
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摘要
水稻种子内生细菌具有多样的生物学功能,可以影响根系的生长与发育。通过砂培法比较分析了3株内生细菌对黄华占水稻根系活力、酶活及鲜质量和干质量的影响。结果表明,3株内生细菌对黄华占根系鲜质量/干质量、根系活力、SOD/POD/CAT酶活性的影响不完全一致,但总体而言都具有积极的作用,其中菌株OH2的促生效果最好。
Endophytic bacteria in seeds have diverse biological functions,which could affect growth and development of roots.Effects of 3 endophytic bacteria on Huanghuazhan's root activity,enzyme activity,fresh weight and dry weight were analyzed by sand culture experiment. Results showed that effects of 3 endophytic bacteria on root fresh weight/dry weight,root activity and SOD/POD/CAT enzyme activity of Huanghuazhan were not identical,but all of them had positive effects on whole,effect of strain OH2 was the best.
Endophytic bacteria in seeds have diverse biological functions,which could affect growth and development of roots.Effects of 3 endophytic bacteria on Huanghuazhan's root activity,enzyme activity,fresh weight and dry weight were analyzed by sand culture experiment. Results showed that effects of 3 endophytic bacteria on root fresh weight/dry weight,root activity and SOD/POD/CAT enzyme activity of Huanghuazhan were not identical,but all of them had positive effects on whole,effect of strain OH2 was the best.
引文
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[2]陈龙,梁子宁,朱华.植物内生菌研究进展[J].生物技术通报,2015,31(8):30-34.CHEN Long,LIANG Zining,ZHU Hua. Research advances in the studies of plant entophytic[J]. Biotechnology Bulletin,2015,31(8):30-34.
[3]姚领爱,胡之璧,王莉莉,等.植物内生菌与宿主关系研究进展[J].生态环境学报,2010,19(7):1 750-1 754.YAO Lingai, HU Zhibi, WANG Lili, et al. Research development of the relatioship between plant endophyte and host[J]. Ecology and Environmental Sciences,2010,19(7):1 750-1 754.
[4]刘琳,刘洋,宋未.杂交水稻种子固有细菌群落多样性探究[J].生物技术通报,2009(1):95-99,111.LIU Lin,LIU Yang,SONG Wei. Indigenous bacterial community diversity in hybrid rice(Oryza sativa L.)seed[J]. Biotechnology Bulletin,2009(1):95-99,111.
[5]姜晓宇,高菊生,徐凤花,等.水稻种子内生细菌多样性及其分泌植物生长素能力的测定[J].微生物学报,2013,53(3):269-275.JIANG Xiaoyu,GAO Jusheng, XU Fenghua, et al. Diversity of endophytic bacteria in rice seeds and their secretion of indole acetic acid[J]. Acta Microbiologica Sinica,2013,53(3):269-275.
[6]许明双.番茄和水稻种子可培养内生细菌的多样性分析及促生菌功能研究[D].北京:中国农业大学,2014.XU Mingshuang. Culturable bacterial community compositions from seeds of tomato and rice and function of plant growth promoting endophytic bacteria[D]. Beijing:China Agricultural University,2014.
[7]Saleem M,Arshad M,Hussain S,et al. Perspective of plant growth promoting rhizobacteria(PGPR)containing ACC deaminase in stress agriculture[J]. Journal of Industrial Microbiology&Biotechnology,2007,34(10):635-648
[8]Glick B R,Patten C L,Holguin G,et al. Biochemical and genetic mechanisms used by plant growth promoting bacteria[M]. London:Imperial College Press,1999.
[9]Lugtenberg B,Kamilova F. Plant-growth-promoting rhizobacteria[J]. Annual Review of Microbiology,2009,63(1):541-556.