摘要
该文采用ODS、硅胶、Sephadex LH-20等柱色谱技术,对柬埔寨野生柯拉斯那沉香(Aquilaria crassna)进行了研究。结果表明:从柬埔寨柯拉斯那所产沉香的乙醇提取物中进行分离共得到了10个化合物,包括一对对映异构体(9a/9b)。经波谱解析分别鉴定为6-甲氧基-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮(1)、6-甲氧基-2-[2-(3-甲氧基-4-羟基苯)乙基]色酮(2)、6,7-二甲氧基-2-(2-苯乙基)色酮(3)、6-羟基-2-(2-苯乙基)色酮(4)、6-羟基-2-[2-(4-甲氧基苯)乙基]色酮(5)、8-氯-6-羟基-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮(6)、8-氯-6-羟基-2-[2-(4-甲氧基苯)乙基]色酮(7)、oxidoagarochromone B(8)、4'-demethoxyaqusisnenone D(9)。其中,化合物6、7和9均为首次从柯拉斯那沉香中分离得到。活性测试结果显示,化合物1和2对乙酰胆碱脂酶具有一定的抑制活性,化合物2对人慢性髓原白血病细胞K562具有较弱的抑制作用。
Ten compounds,including one pair of enantiomers (9 a/9 b),were isolated from the ethanol extract of agarwood originating from Aquilaria crassna in Cambodia by comprehensive chromatographic techniques including ODS,silica gel and Sephadex LH-20. On the basis of spectroscopic data,they were identified as 6-methoxy-2-[2- (3-hydroxy-4-methoxyphenyl) ethyl]chromone (1),6-methoxy-2-[2- (3-methoxy-4-hydroxyphenyl) ethyl] chromone (2),6,7-dimethoxy-2- (2-phenylethyl) chromone (3),6-hydroxy-2- (2-phenylethyl) chromone (4),6-hydroxy-2-[2- (4-methoxyphenyl) ethyl ]chromone (5),8-chloro-6-hydroxy-2-[2- (3-hydroxy-4-methoxyphenyl) ethyl]chromone (6),8-chloro-6-hydroxy-2-[2- (4-methoxyphenyl) ethyl] chromone (7),oxidoagarochromone B (8),4'-demethoxyaqusisnenone D (9). Compounds 6,7 and 9 were isolated from agarwood of A. crassna for the first time. Among those,compounds 1 and 2 showed inhibitory activities against acetylcholinesterase. Compound 2 had weak inhibitory effect on K562 cell lines.
引文
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