玉米种质资源抗腐霉茎腐病鉴定
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摘要
腐霉茎腐病(Pythium stalk rot)是玉米生产上的重要病害。2013—2016年,对1213份玉米种质资源进行了抗肿囊腐霉(Pythium inflatum)茎腐病的鉴定与评价。在1213份玉米种质中,鉴定出高抗肿囊腐霉茎腐病的材料207份,占鉴定总数的17.1%,主要来自中国的内蒙古、河北、山西及美国等地。抗性材料159份,占鉴定材料数的13.1%,主要由源自中国的内蒙古、云南、山西和美国等地的种质构成。由此可见,玉米种质中存在较为丰富的抗腐霉茎腐病资源,且抗性水平与地理来源有关。自交系和农家种中对肿囊腐霉茎腐病表现高抗的种质分别占鉴定种质总数的18.7%和10.6%,表明自交系中高抗肿囊腐霉茎腐病资源较农家种更为丰富。
Pythium stalk rot is a serious disease in maize production. A total of 1213 maize accessions were screened for resistance to Pythium stalk rot from 2013 to 2016. Among the 1213 germplasm resources, 207 were highly resistant to Pythium stalk rot, accounting for 17.1% of total accessions screened, mainly from Inner Mongolia, Hebei, Shanxi Provinces of China and the United States, 159 were resistant to Pythium stalk rot, accounting for 13.1% of total accessions, mainly from Inner Mongolia, Yunnan, Shanxi Provinces of China and USA, showing that there are relatively abundant germplasm resistant to Pythium stalk rot in these maize accessions, and the level of resistance is related to geographical origin. In present study, 18.7% of maize inbred lines were highly resistant to Pythium stalk rot, whereas 10.6% of landraces were highly resistant to the same diseases, indicating higher level of resistance and more multiple resistance were in inbred lines than in landraces.
Pythium stalk rot is a serious disease in maize production. A total of 1213 maize accessions were screened for resistance to Pythium stalk rot from 2013 to 2016. Among the 1213 germplasm resources, 207 were highly resistant to Pythium stalk rot, accounting for 17.1% of total accessions screened, mainly from Inner Mongolia, Hebei, Shanxi Provinces of China and the United States, 159 were resistant to Pythium stalk rot, accounting for 13.1% of total accessions, mainly from Inner Mongolia, Yunnan, Shanxi Provinces of China and USA, showing that there are relatively abundant germplasm resistant to Pythium stalk rot in these maize accessions, and the level of resistance is related to geographical origin. In present study, 18.7% of maize inbred lines were highly resistant to Pythium stalk rot, whereas 10.6% of landraces were highly resistant to the same diseases, indicating higher level of resistance and more multiple resistance were in inbred lines than in landraces.
引文
[1]王晓鸣,吴全安,刘晓娟,马国忠.寄生玉米的6种腐霉及其致病性研究.植物病理学报,1994,24:343–346Wang X M,Wu Q A,Liu X J,Ma G Z.Identification and pathogenicity of Pythium spp.isolated from maize.Acta Phytopathol Sin,1994,24:343–346(in Chinese with English abstract)
[2]吴全安,梁克恭,朱小阳,王晓鸣,金加同,王桂跃.北京和浙江地区玉米青枯病病原菌的分离与鉴定.中国农业科学,1989,11:71–75Wu Q A,Liang K G,Zhu X Y,Wang X M,Jin J T,Wang G Y.Isolation and identification of the pathogen of maize stalk rot in Beijing and Zhejiang.Sci Agric Sin,1989,11:71–75(in Chinese with English abstract)
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[4]Shin J H,Han J H,Lee J K,Kim K S.Characterization of the maize stalk rot pathogens Fusarium subglutinans and F.temperatum and the effect of fungicides on their mycelial growth and colony formation.Plant Pathol J,2014,30:397–406
[5]王晓鸣,晋齐鸣,石洁,王作英,李晓.玉米病害发生现状与推广品种抗性对未来病害发展的影响.植物病理学报,2006,36:1–11Wang X M,Jin Q M,Shi J,Wang Z Y,Li X.The status of maize diseases and the possible effect of variety resistance on disease occurrence in the future.Acta Phytopathol Sin,2006,36:1–11(in Chinese with English abstract)
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[7]段灿星,王晓鸣,武小菲,杨知还,宋凤景,赵立萍,孙素丽,朱振东.玉米种质和新品种对腐霉茎腐病和镰孢穗腐病的抗性分析.植物遗传资源学报,2015,16:947–954Duan C X,Wang X M,Wu X F,Yang Z H,Song F J,Zhao L P,Sun S L,Zhu Z D.Analysis of maize accessions resistance to Pythium stalk rot and Fusarium ear rot.J Plant Genet Resour,2015,16:947–954(in Chinese with English abstract)
[8]王富荣,石秀清.玉米品种抗茎腐病鉴定.植物保护学报,2000,24:59–62Wang F R,Shi X Q.Identification for resistance of maize cultivars to stalk rot.J Plant Prot,2000,24:59–62(in Chinese with English abstract)
[9]宋燕春,裴二芹,石云素,王天宇,黎裕.玉米重要自交系的肿囊腐霉茎腐病抗性鉴定与评价.植物遗传资源学报,2012,13:798–802Song Y C,Pei E Q,Shi Y S,Wang T Y,Li Y.Identification and evaluation of resistance to stalk rot(Pythium inflatum Matthews)in important inbred lines of maize.J Plant Genet Resour,2012,13:798–802(in Chinese with English abstract)
[10]Young H C.The toothpick method of inoculating corn for ear and stalk rot.Phytopathology,1943,33:16
[11]宋佐衡,陈捷,刘伟成,咸洪全,孙秀华.玉米茎腐病接种方法比较.植物保护,1993,19:37–38Song Z H,Chen J,Liu W C,Xian H Q,Sun X H.Comparison of inoculation method for maize stalk rot.Plant Prot,1993,19:37–38(in Chinese)
[12]王晓鸣,石洁,晋齐鸣,李晓,孙世贤.玉米病虫害田间手册.北京:中国农业科学技术出版社,2010.p 259Wang X M,Shi J,Jin Q M,Li X,Sun S X.Field Manual of Maize Diseases and Pests.Beijing:China Agriculture Science and Technology Press,2010.p 259(in Chinese)
[13]黄天述,叶华智,王晓鸣,唐莉,王晓梅,金庆超.玉米种质资源对纹枯病的抗性鉴定与评价.植物遗传资源学报,2005,6:291–294Huang T S,Ye H Z,Wang X M,Tang L,Wang X M,Jin Q C.Identification and evaluation of maize germplasm for resistance to corn sheath blight(Rhizoctonia solani).J Plant Genet Resour,2005,6:291–294(in Chinese with English abstract)
[14]吴全安,朱小阳,林宏旭,金加同,王桂跃.玉米青枯病病原菌的分离及其致病性测定技术的研究.植物病理学报,1997,27:29–35Wu Q A,Zhu X Y,Lin H X,Jin J T,Wang G Y.On the technique of isolation and the pathogenicity of the maize stalk rot pathogens.Acta Phytopathol Sin,1997,27:29–35(in Chinese with English abstract)
[15]程伟东,李石初,覃兰秋,谭贤杰,吴子恺.广西玉米种质资源对纹枯病的抗性鉴定.植物遗传资源学报,2009,10:566–571Cheng W D,Li S C,Qin L Q,Tan X J,Wu Z K.Identification of resistance to sheath blight caused by Rhizoctonia solani in maize germplasm in Guangxi.J Plant Genet Resour,2009,10:566–571(in Chinese with English abstract)
[16]郭成,郭满库,徐生军,魏宏玉,金社林,赵峰.农家种玉米资源抗丝黑穗病鉴定与评价.玉米科学,2016,24(1):156–159Guo C,Guo M K,Xu S J,Wei H Y,Jin S L,Zhao F.Identification and evaluation of maize peasant resources resistance to Sporosporium reilianum.J Maize Sci,2016,24(1):156–159(in Chinese with English abstract)
[2]吴全安,梁克恭,朱小阳,王晓鸣,金加同,王桂跃.北京和浙江地区玉米青枯病病原菌的分离与鉴定.中国农业科学,1989,11:71–75Wu Q A,Liang K G,Zhu X Y,Wang X M,Jin J T,Wang G Y.Isolation and identification of the pathogen of maize stalk rot in Beijing and Zhejiang.Sci Agric Sin,1989,11:71–75(in Chinese with English abstract)
[3]陈捷.我国玉米穗、茎腐病病害研究现状与展望.沈阳农业大学学报,2000,31:393–401Chen J.Status and perspective on research of ear rot and stalk rot in maize.J Shenyang Agric Univ,2000,31:393–401(in Chinese with English abstract)
[4]Shin J H,Han J H,Lee J K,Kim K S.Characterization of the maize stalk rot pathogens Fusarium subglutinans and F.temperatum and the effect of fungicides on their mycelial growth and colony formation.Plant Pathol J,2014,30:397–406
[5]王晓鸣,晋齐鸣,石洁,王作英,李晓.玉米病害发生现状与推广品种抗性对未来病害发展的影响.植物病理学报,2006,36:1–11Wang X M,Jin Q M,Shi J,Wang Z Y,Li X.The status of maize diseases and the possible effect of variety resistance on disease occurrence in the future.Acta Phytopathol Sin,2006,36:1–11(in Chinese with English abstract)
[6]王晓鸣,戴法超,朱振东,焦志亮,杨家秀,李晓,郭满库.玉米自交系和杂交种的抗病特性研究.中国农业科学,2000,33:132–140Wang X M,Dai F C,Zhu Z D,Jiao Z L,Yang J X,Li X,Guo M K.Characteristics of disease-resistance of maize inbred lines and hybrids.Sci Agric Sin,2000,33:132–140(in Chinese with English abstract)
[7]段灿星,王晓鸣,武小菲,杨知还,宋凤景,赵立萍,孙素丽,朱振东.玉米种质和新品种对腐霉茎腐病和镰孢穗腐病的抗性分析.植物遗传资源学报,2015,16:947–954Duan C X,Wang X M,Wu X F,Yang Z H,Song F J,Zhao L P,Sun S L,Zhu Z D.Analysis of maize accessions resistance to Pythium stalk rot and Fusarium ear rot.J Plant Genet Resour,2015,16:947–954(in Chinese with English abstract)
[8]王富荣,石秀清.玉米品种抗茎腐病鉴定.植物保护学报,2000,24:59–62Wang F R,Shi X Q.Identification for resistance of maize cultivars to stalk rot.J Plant Prot,2000,24:59–62(in Chinese with English abstract)
[9]宋燕春,裴二芹,石云素,王天宇,黎裕.玉米重要自交系的肿囊腐霉茎腐病抗性鉴定与评价.植物遗传资源学报,2012,13:798–802Song Y C,Pei E Q,Shi Y S,Wang T Y,Li Y.Identification and evaluation of resistance to stalk rot(Pythium inflatum Matthews)in important inbred lines of maize.J Plant Genet Resour,2012,13:798–802(in Chinese with English abstract)
[10]Young H C.The toothpick method of inoculating corn for ear and stalk rot.Phytopathology,1943,33:16
[11]宋佐衡,陈捷,刘伟成,咸洪全,孙秀华.玉米茎腐病接种方法比较.植物保护,1993,19:37–38Song Z H,Chen J,Liu W C,Xian H Q,Sun X H.Comparison of inoculation method for maize stalk rot.Plant Prot,1993,19:37–38(in Chinese)
[12]王晓鸣,石洁,晋齐鸣,李晓,孙世贤.玉米病虫害田间手册.北京:中国农业科学技术出版社,2010.p 259Wang X M,Shi J,Jin Q M,Li X,Sun S X.Field Manual of Maize Diseases and Pests.Beijing:China Agriculture Science and Technology Press,2010.p 259(in Chinese)
[13]黄天述,叶华智,王晓鸣,唐莉,王晓梅,金庆超.玉米种质资源对纹枯病的抗性鉴定与评价.植物遗传资源学报,2005,6:291–294Huang T S,Ye H Z,Wang X M,Tang L,Wang X M,Jin Q C.Identification and evaluation of maize germplasm for resistance to corn sheath blight(Rhizoctonia solani).J Plant Genet Resour,2005,6:291–294(in Chinese with English abstract)
[14]吴全安,朱小阳,林宏旭,金加同,王桂跃.玉米青枯病病原菌的分离及其致病性测定技术的研究.植物病理学报,1997,27:29–35Wu Q A,Zhu X Y,Lin H X,Jin J T,Wang G Y.On the technique of isolation and the pathogenicity of the maize stalk rot pathogens.Acta Phytopathol Sin,1997,27:29–35(in Chinese with English abstract)
[15]程伟东,李石初,覃兰秋,谭贤杰,吴子恺.广西玉米种质资源对纹枯病的抗性鉴定.植物遗传资源学报,2009,10:566–571Cheng W D,Li S C,Qin L Q,Tan X J,Wu Z K.Identification of resistance to sheath blight caused by Rhizoctonia solani in maize germplasm in Guangxi.J Plant Genet Resour,2009,10:566–571(in Chinese with English abstract)
[16]郭成,郭满库,徐生军,魏宏玉,金社林,赵峰.农家种玉米资源抗丝黑穗病鉴定与评价.玉米科学,2016,24(1):156–159Guo C,Guo M K,Xu S J,Wei H Y,Jin S L,Zhao F.Identification and evaluation of maize peasant resources resistance to Sporosporium reilianum.J Maize Sci,2016,24(1):156–159(in Chinese with English abstract)