喜勒可吸收生物修复膜治疗下颌角区骨缺损实验研究
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摘要
目的本研究旨在对比喜勒可吸收生物修复膜与市售生物修复膜修复骨缺损的效果。方法12只兔子分为2组:喜勒可吸收生物修复膜组6只(左侧植入自体骨覆盖喜勒可吸收生物膜,右侧不植骨不覆膜),市售生物膜组6只(左侧不植骨不覆膜,右侧植入自体骨覆盖市售生物膜),在生理盐水充分冷却的条件下,采用直径为6 mm的球钻在兔下颌骨左右两侧下颌角区分别制备直径6 mm洞穿性的骨缺损。覆盖生物膜,需超出缺损边缘2 mm,分层缝合创口。术后3 d每天皮下注射抗生素。分别于术后1个月、3个月采用空气栓塞法处死实验兔,取整个下颌骨。结果 X线及组织学检查结果显示,术后1个月覆膜的动物骨缺损有不同程度修复,而不覆盖膜的骨缺损均明显存在,且骨缺损内有肌肉长入,但喜勒可吸收生物修复膜组具有完整的生物膜结构。术后3个月覆膜的骨缺损区均有新骨形成,而不覆膜缺损区被结缔组织填充,没有新骨形成。结论喜勒可吸收生物膜与市售生物修复膜均能有效阻挡软组织长入骨缺损区,作为骨细胞载体促进骨修复,但喜勒可吸收生物修复膜有较强的抗降解能力。
Objective To compare the effect of Healer bioresorbable collagen membrane and bio-membrane from the market for bone defect in rabbits. Methods A 6 mm in diameter bone defect was induced in bilateral mandible angle of 12 New Zealand rabbits,those 12 rabbits were divided into two groups. The defect of mandible angle of 6 New Zealand rabbits were covered laterally and medially by Healer bioresorbable collagen membrane( left side) and the right side as blank control don't cover anything. The defect of mandible angle of 6 New Zealand rabbits were covered by bio-membrane from the market( right side),the left side as blank control don't cover anything. Six rabbits were sacrificed in the first months,and the others were sacrificed in the third month. Results The X-ray and histological examination showed that in the first month the bone defect was come to repaired and the Healer group had complete biomembrane structure,the bone defect was still evident in the blank control group; in the third month all the membrane-covered partly defects healed with new formed bone,while no new bone was formed in control group,but the defects were filled with soft tissue. Conclusion Healer bioresorbable collagen membrane and bio-membrane from the market can effectively block off soft tissue and serve as osteocyte scaffold to promote the healing of the bone defects. The Healer bioresorbable collagen membrane has stronger anti-degradation ability.
Objective To compare the effect of Healer bioresorbable collagen membrane and bio-membrane from the market for bone defect in rabbits. Methods A 6 mm in diameter bone defect was induced in bilateral mandible angle of 12 New Zealand rabbits,those 12 rabbits were divided into two groups. The defect of mandible angle of 6 New Zealand rabbits were covered laterally and medially by Healer bioresorbable collagen membrane( left side) and the right side as blank control don't cover anything. The defect of mandible angle of 6 New Zealand rabbits were covered by bio-membrane from the market( right side),the left side as blank control don't cover anything. Six rabbits were sacrificed in the first months,and the others were sacrificed in the third month. Results The X-ray and histological examination showed that in the first month the bone defect was come to repaired and the Healer group had complete biomembrane structure,the bone defect was still evident in the blank control group; in the third month all the membrane-covered partly defects healed with new formed bone,while no new bone was formed in control group,but the defects were filled with soft tissue. Conclusion Healer bioresorbable collagen membrane and bio-membrane from the market can effectively block off soft tissue and serve as osteocyte scaffold to promote the healing of the bone defects. The Healer bioresorbable collagen membrane has stronger anti-degradation ability.
引文
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[5]赵进峰,尹蒙熔,樊马娟.改良式GBR技术联合即刻种植术后即刻修复上前牙的疗效分析[J].黑龙江医药科学,2015,38(4):151-152.
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[12]侯劲松,黄洪章.脱细胞真皮基质在口腔医学领域的应用进展[J].中国口腔颌面外科杂志,2007,5(3):163-169.
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[15]杜晓丹,方玉,奚廷斐,等.动物源性胶原的生产、应用及其免疫原性[J].中国组织工程研究与临床康复,2008,12(23):4511-4514.
[16]李新明,刘欢,刘洁.基于多肽及多肽衍生物而构建的具有生物应用潜力的超分子自组装体的研究[C].苏州:全国高分子学术论文报告会,2015.
[17]周艺群.可吸收性GTR膜材料的研究现状与进展[J].中国口腔种植学杂志,2002,7(1):42-45.
[18]Zhang Y,Zhang X,Shi B,et al.Membranes for guided tissue and bone regeneration[J].Oral Maxillofac Surg Clin North Am,2013,1(1):1-10.
[19]宋爱梅,杨丕山,孙钦峰,等.屏障膜放置时间与牙周再生量关系的动物实验研究[J].牙体牙髓牙周病学杂志,2001,11(1):17-19.
[20]Karring T,Nyman S,Lindhe J,et al.Potentials for root resorption during periodontal wound healing[J].J Clin Periodontol,1984,11(1):41-52.
[2]Talreja PS,Gayathri GV,Mehta DS.Treatment of an early failing implant by guided bone regeneration using resorbable collagenmembrane and bioactive glass[J].J Indian Soc Periodontol,2013,17(1):131-136.
[3]郑汉峰.不同口腔修复膜材料在牙种植引导骨再生中的应用效果观察[J].临床医学工程,2016,23(8):1001-1002.
[4]蒋柳宏,董滢,景向东.引导骨再生技术在上前牙种植修复中的效果[J].中国组织工程研究,2015,19(7):1138-1142.
[5]赵进峰,尹蒙熔,樊马娟.改良式GBR技术联合即刻种植术后即刻修复上前牙的疗效分析[J].黑龙江医药科学,2015,38(4):151-152.
[6]黄宇文.牙周炎对种植体周围边缘骨吸收的影响[J].实用临床医学,2017,18(7):63-64,70.
[7]Mercado KP,Helguera M,Hocking DC,et al.Noninvasive quantitative imaging of collagen microstructure in threedimensional hydrogels using high-frequency ultrasound[J].Tissue Eng Part C Methods,2015,21(7):671-682.
[8]胡秀莲,邱立新,林野,等.可吸收性Bio-Gide膜治疗下颌角区局部骨缺损实验研究[J].中国口腔种植学杂志,2002,7(1):10-12,24.
[9]马遥.静电纺PBS/PPC载rh-BMP2纳米纤维膜的制备及生物活性评价[D].长春:吉林大学,2015.
[10]白冰,朱静涛,王立威.作为GBR屏障膜的新型胶原膜的体内植入效果分析[J].口腔医学,2015,35(3):170-174.
[11]杜倩.壳聚糖-胶原角膜修复材料的制备及生物相容性[J].中国组织工程研究,19(52):8433-8437.
[12]侯劲松,黄洪章.脱细胞真皮基质在口腔医学领域的应用进展[J].中国口腔颌面外科杂志,2007,5(3):163-169.
[13]Takami Y,Matsuda T,Yoshitake M,et al.Dispase/detergent treated dermal matrix as a dermal substitute[J].Burns,22(3):182-190.
[14]Livesey SA,Herndon DN,Hollyoak MA,et al.Transplanted acellular allograft dermal matrix:potential as a template for the reconstruction of viable dermis[J].Transplantation,1998,60(1):1-9.
[15]杜晓丹,方玉,奚廷斐,等.动物源性胶原的生产、应用及其免疫原性[J].中国组织工程研究与临床康复,2008,12(23):4511-4514.
[16]李新明,刘欢,刘洁.基于多肽及多肽衍生物而构建的具有生物应用潜力的超分子自组装体的研究[C].苏州:全国高分子学术论文报告会,2015.
[17]周艺群.可吸收性GTR膜材料的研究现状与进展[J].中国口腔种植学杂志,2002,7(1):42-45.
[18]Zhang Y,Zhang X,Shi B,et al.Membranes for guided tissue and bone regeneration[J].Oral Maxillofac Surg Clin North Am,2013,1(1):1-10.
[19]宋爱梅,杨丕山,孙钦峰,等.屏障膜放置时间与牙周再生量关系的动物实验研究[J].牙体牙髓牙周病学杂志,2001,11(1):17-19.
[20]Karring T,Nyman S,Lindhe J,et al.Potentials for root resorption during periodontal wound healing[J].J Clin Periodontol,1984,11(1):41-52.