Clus-human蛋白在精神分裂症患者脑脊液中的差异表达
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摘要
目的:分析精神分裂症患者脑脊液中蛋白质的差异表达。方法:选取精神分裂症及非精神分裂症患者各12例,抽取脑脊液,用蛋白质分离技术——二维聚丙烯酰氨凝胶技术和蛋白质识别与鉴定技术——生物质谱技术筛选脑脊液中标志性蛋白质。结果:精神分裂症组Clus-human蛋白含量为(2.560 6±0.319 2)μg/L,对照组Clus-human蛋白含量为(2.134 9±0.418 7)μg/L,精神分裂症蛋白Clus-human含量与对照组Clus-human含量具有统计学差异(t=2.801,P=0.010)。结论:精神分裂症患者脑脊液中发现Clus-human蛋白,对精神分裂症的诊断可能具有重要意义。
Objective:To investigate the differential expression of proteins in the cerebrospinal fluid of patients with schizophrenia.Methods:A total of 12 patients with schizophrenia and 12 patients without schizophrenia were enrolled. Cerebrospinal fluid was collected,and two-dimensional polyacrylamide gel electrophoresis for protein separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for protein recognition and identification were used to screen out symbolic proteins in cerebrospinal fluid. Results:There was a significant difference in the content of Clus-human protein between the schizophrenia group and the nonschizophrenia group(2.560 6±0.319 2 μg/L vs. 2.134 9±0.418 7 μg/L,t=2.801,P=0.010). Conclusion:Clus-human protein is found in the cerebrospinal fluid of patients with schizophrenia and may have important significance in the diagnosis of schizophrenia.
Objective:To investigate the differential expression of proteins in the cerebrospinal fluid of patients with schizophrenia.Methods:A total of 12 patients with schizophrenia and 12 patients without schizophrenia were enrolled. Cerebrospinal fluid was collected,and two-dimensional polyacrylamide gel electrophoresis for protein separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for protein recognition and identification were used to screen out symbolic proteins in cerebrospinal fluid. Results:There was a significant difference in the content of Clus-human protein between the schizophrenia group and the nonschizophrenia group(2.560 6±0.319 2 μg/L vs. 2.134 9±0.418 7 μg/L,t=2.801,P=0.010). Conclusion:Clus-human protein is found in the cerebrospinal fluid of patients with schizophrenia and may have important significance in the diagnosis of schizophrenia.
引文
[1] Ricolleau G,Charbonnel C,LodéL,et al. Surface-enhanced laser desorption/ionization time of flight mass spectrometry protein profiling identifies ubiquitin and ferritin light chain as prognostic biomarkers in node-negative breast cancer tumors[J]. Protemics,2006,6(6):1963-1975.
[2]管丽丽,杜立哲,马弘.精神分裂症的疾病负担(综述)[J].中国心理卫生杂志,2012,26(12):913-919.
[3] World Health Organization. The global burden of disease:2004 update[R/OL]. http://www.who.int/healthinfo/global_burden_disease/2004_report_update/en/.
[4] Zhou N,Wang J,Yu Y,et al. Mass spectrum analysis of serum biomarker proteins from patients with schizophrenia[J]. Biomedical Chromatography,2014,28(5):654-659.
[5]刘建君,白克镇.精神分裂症的蛋白组学研究新进展[J].神经疾病与精神卫生,2008,8(2):153-155.
[6]刘建君,白克镇.应用SELDI-TOF-MS技术筛查精神分裂症血清蛋白标志物[J].上海精神医学,2010,22(3):136-139.
[7] Athanas KM,Mauney SL,Woo TW. Increased extracellular clusterin in the prefrontal cortex in schizophrenia[J]. Schizophr Res,2015,169(1-3):381-385.
[8] Yu JT,Tan L. The role of clusterin in Alzheimer’s disease:pathways,pathogenesis,and therapy[J]. Mol Neurobiol,2012,45(2):314-326.
[9] Li H,Zhang X,Guo Y,et al. A case-control study ofgenetic assoei·ation of the PLA2G4A gene with schizophrenia in a Chinese population[J]. Psychiatr Genet,2007,17(3):205.
[10] Steiner J,Bielau H,Bemstein HG,et al. Increased cerebrospinal fluid and serum levels of S100B in first-onset schizophrenia are not related to a degenerative release of glial fibrillar acidic protein,myelin basic protein and neurone-specific enolase from glia or neurones[J]. J Neurol Neurosurg Psychiatry,2006,77(11):1284-1287.
[11] Franck N,Georgieff N. Neurodevelopmental hypothesis of schizophrenia[J]. Rev Prat,2002,52(11):1202-1207.
[12] Archer T. Neurodegeneration in schizophrenia[J]. Expert Rev Neurother,2010,10(7):1131-1141.
[13] Aghajanian GK,Marek GJ. Serotonin model of schizoph-reni-emerging role of glutamate mechanisms[J]. Brain Res Brain Res Rev,2000,31(2/3):302-312.
[14] Brown AS,Derkits EJ. Prenatal infection and schizophrenia:a review of epidemiologic and translational studies[J]. Am J Psychiatry,2010,167(3):261-280.
[15]刘辉,邵岩华,王家学.精神分裂症血清中自身抗体与Fas诱导因子活性关联[J].免疫学杂志,2010,26(2):136-139.
[16] Strous RD,Shoenfeld Y. Schizophrenia,autoimmunity and immune system dysregulation:a comprehensive model up-dated and revisited[J].J Autoimmun,2006,27(2):71-80.
[17] Roth TL,Lubin FD,Sodhi M,et al. Epigenetic mechanisms in schizophrenia[J]. Biochim Biophys Acta,2009,1790(9):869-877.
[18] Jones SE,Jomary C. Clusterin[J]. Int J Biochem Cell Biol,2002,34(5):427-431.
[2]管丽丽,杜立哲,马弘.精神分裂症的疾病负担(综述)[J].中国心理卫生杂志,2012,26(12):913-919.
[3] World Health Organization. The global burden of disease:2004 update[R/OL]. http://www.who.int/healthinfo/global_burden_disease/2004_report_update/en/.
[4] Zhou N,Wang J,Yu Y,et al. Mass spectrum analysis of serum biomarker proteins from patients with schizophrenia[J]. Biomedical Chromatography,2014,28(5):654-659.
[5]刘建君,白克镇.精神分裂症的蛋白组学研究新进展[J].神经疾病与精神卫生,2008,8(2):153-155.
[6]刘建君,白克镇.应用SELDI-TOF-MS技术筛查精神分裂症血清蛋白标志物[J].上海精神医学,2010,22(3):136-139.
[7] Athanas KM,Mauney SL,Woo TW. Increased extracellular clusterin in the prefrontal cortex in schizophrenia[J]. Schizophr Res,2015,169(1-3):381-385.
[8] Yu JT,Tan L. The role of clusterin in Alzheimer’s disease:pathways,pathogenesis,and therapy[J]. Mol Neurobiol,2012,45(2):314-326.
[9] Li H,Zhang X,Guo Y,et al. A case-control study ofgenetic assoei·ation of the PLA2G4A gene with schizophrenia in a Chinese population[J]. Psychiatr Genet,2007,17(3):205.
[10] Steiner J,Bielau H,Bemstein HG,et al. Increased cerebrospinal fluid and serum levels of S100B in first-onset schizophrenia are not related to a degenerative release of glial fibrillar acidic protein,myelin basic protein and neurone-specific enolase from glia or neurones[J]. J Neurol Neurosurg Psychiatry,2006,77(11):1284-1287.
[11] Franck N,Georgieff N. Neurodevelopmental hypothesis of schizophrenia[J]. Rev Prat,2002,52(11):1202-1207.
[12] Archer T. Neurodegeneration in schizophrenia[J]. Expert Rev Neurother,2010,10(7):1131-1141.
[13] Aghajanian GK,Marek GJ. Serotonin model of schizoph-reni-emerging role of glutamate mechanisms[J]. Brain Res Brain Res Rev,2000,31(2/3):302-312.
[14] Brown AS,Derkits EJ. Prenatal infection and schizophrenia:a review of epidemiologic and translational studies[J]. Am J Psychiatry,2010,167(3):261-280.
[15]刘辉,邵岩华,王家学.精神分裂症血清中自身抗体与Fas诱导因子活性关联[J].免疫学杂志,2010,26(2):136-139.
[16] Strous RD,Shoenfeld Y. Schizophrenia,autoimmunity and immune system dysregulation:a comprehensive model up-dated and revisited[J].J Autoimmun,2006,27(2):71-80.
[17] Roth TL,Lubin FD,Sodhi M,et al. Epigenetic mechanisms in schizophrenia[J]. Biochim Biophys Acta,2009,1790(9):869-877.
[18] Jones SE,Jomary C. Clusterin[J]. Int J Biochem Cell Biol,2002,34(5):427-431.