胺化松香基树脂对牛奶中青霉素类抗生素的吸附性能研究
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摘要
文章以实验室自制的胺化松香基树脂(ethylenediamine rosin-based resin,EDAR)为吸附材料,通过静态吸附实验测试其对牛奶中青霉素类抗生素的去除效果,采用液相色谱-串联质谱法进行浓度测定,并研究了树脂的重复使用能力,实验结果表明,EDAR对牛奶中的阿莫西林和青霉素G都具有一定的吸附效果。EDAR对阿莫西林和青霉素G的等温吸附过程均更符合Langmuir等温吸附模型,动力学过程符合准二级动力学模型。胺化树脂可用作固相萃取柱填料,对牛奶中抗生素的残留检测具有潜在的应用前景。
Using the ethylenediamine rosin-based resin(EDAR) as the adsorption material, the effect of the removal of penicillins in milk was tested by static adsorption experiment. The concentration of penicillins was measured by LC-MS-MS method and the reuse of the resin was also studied. The experimental results showed that EDAR has a certain adsorption effect on Amoxicillin and penicillin G in milk. The adsorption process of amoxicillin and penicillin G on EDAR is accorded with the Langmuir isothermal adsorption model, and the kinetic process is accorded with the pseudo-second model. EDAR can be used as a filler of solid phase extraction column, which has potential prospects for the detection of antibiotic residues in milk.
Using the ethylenediamine rosin-based resin(EDAR) as the adsorption material, the effect of the removal of penicillins in milk was tested by static adsorption experiment. The concentration of penicillins was measured by LC-MS-MS method and the reuse of the resin was also studied. The experimental results showed that EDAR has a certain adsorption effect on Amoxicillin and penicillin G in milk. The adsorption process of amoxicillin and penicillin G on EDAR is accorded with the Langmuir isothermal adsorption model, and the kinetic process is accorded with the pseudo-second model. EDAR can be used as a filler of solid phase extraction column, which has potential prospects for the detection of antibiotic residues in milk.
引文
[1]张丽茹,黄新红,张东丽,等.鲜牛奶中抗生素残留的原因及常用检测方法[J].现代畜牧科技,2018(1):109.
[2]李丹妮,贡松松,顾欣,等.生鲜牛乳中抗生素残留检测技术研究进展[J].中国乳品工业,2017,45(5):38-42,58.
[3]李小会,李海星,段亚军,等.免疫分析技术在乳及乳制品抗生素类兽药残留检测中的应用[J].食品安全质量检测学报,2018(4):776-780.
[4]Takagi R,Fukuda J,Nagata K,et al.A microfluidic microbial culture device for rapid determination of the minimum inhibitory concentration of antibiotics[J].Analyst,2013,138(4):1000-1003.
[5]范维,高晓月,李贺楠,等.高通量微生物显色法快速检测动物源性食品中抗生素残留[J].食品科学,2017,38(16):239-244.
[6]王浩,赵丽,杨红梅,等.液相色谱-串联质谱法测定牛奶中35种四环素类、磺胺类、青霉素类、大环内酯类、氯霉素类抗生素残留[J].色谱,2015,33(9):995-1001.
[7]Luzardo O P,Ruiz-Suárez N,Almeida-González M,et al.Multi-residue method for the determination of 57 persistent organic pollutants in human milk and colostrum using a Qu ECh ERS-based extraction procedure[J].Analytical and Bioanalytical Chemistry,2013,405(29):9523-9536.
[8]Huet A C,Charlier C,Tittlemier S A,et al.Simultaneous determination of(fluoro)quinolone antibiotics in kidney,marine products,eggs,and muscle by enzyme-linked immunosorbent assay(ELISA)[J].Journal of Agricultural and Food Chemistry,2006,54(8):2822-2827.
[9]马亚红,黄婉婷,刁开盛,等.氨化松香基交联聚合树脂对水中诺氟沙星的吸附性能[J].环境科学,2018,39(1):161-169.
[10]GB/T 22975-2008.牛奶和奶粉中阿莫西林、氨苄西林、哌拉西林、青霉素G、青霉素V、苯唑西林、氯唑西林、萘夫西林和双氯西林残留量的测定液相色谱-串联质谱法[S].北京:中国标准出版社,2013.
[2]李丹妮,贡松松,顾欣,等.生鲜牛乳中抗生素残留检测技术研究进展[J].中国乳品工业,2017,45(5):38-42,58.
[3]李小会,李海星,段亚军,等.免疫分析技术在乳及乳制品抗生素类兽药残留检测中的应用[J].食品安全质量检测学报,2018(4):776-780.
[4]Takagi R,Fukuda J,Nagata K,et al.A microfluidic microbial culture device for rapid determination of the minimum inhibitory concentration of antibiotics[J].Analyst,2013,138(4):1000-1003.
[5]范维,高晓月,李贺楠,等.高通量微生物显色法快速检测动物源性食品中抗生素残留[J].食品科学,2017,38(16):239-244.
[6]王浩,赵丽,杨红梅,等.液相色谱-串联质谱法测定牛奶中35种四环素类、磺胺类、青霉素类、大环内酯类、氯霉素类抗生素残留[J].色谱,2015,33(9):995-1001.
[7]Luzardo O P,Ruiz-Suárez N,Almeida-González M,et al.Multi-residue method for the determination of 57 persistent organic pollutants in human milk and colostrum using a Qu ECh ERS-based extraction procedure[J].Analytical and Bioanalytical Chemistry,2013,405(29):9523-9536.
[8]Huet A C,Charlier C,Tittlemier S A,et al.Simultaneous determination of(fluoro)quinolone antibiotics in kidney,marine products,eggs,and muscle by enzyme-linked immunosorbent assay(ELISA)[J].Journal of Agricultural and Food Chemistry,2006,54(8):2822-2827.
[9]马亚红,黄婉婷,刁开盛,等.氨化松香基交联聚合树脂对水中诺氟沙星的吸附性能[J].环境科学,2018,39(1):161-169.
[10]GB/T 22975-2008.牛奶和奶粉中阿莫西林、氨苄西林、哌拉西林、青霉素G、青霉素V、苯唑西林、氯唑西林、萘夫西林和双氯西林残留量的测定液相色谱-串联质谱法[S].北京:中国标准出版社,2013.