舒糖宝口服液治疗2型糖尿病小鼠模型的效果观察
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摘要
目的建立2型糖尿病小鼠模型,观察中药舒糖宝口服液对其血糖、血脂及尿素含量的影响。方法 35只昆明种小鼠给予高糖、高脂饮食4周后腹腔注射链佐霉素(STZ)建立2型糖尿病模型,随机分为模型组11只、舒糖宝组12只、二甲双胍组12只,另取10只正常小鼠为对照组。舒糖宝组、二甲双胍组分别给予舒糖宝口服液和二甲双胍灌服,对照组与模型组给予蒸馏水灌服。观察并记录各组小鼠饮水情况;检测给药前后各组小鼠血糖、总胆固醇(TC)、三酰甘油(TG)、尿素(Urea)含量。结果给药后,舒糖宝组、二甲双胍组小鼠每天饮水量少于对照组、模型组(P<0.01);舒糖宝组、二甲双胍组小鼠血糖、TG含量低于模型组(P<0.01);降糖率分别达42.50%和35.09%。结论舒糖宝口服液能有效降低2型糖尿病小鼠血糖、三酰甘油及饮水量。
Objective To establish a mouse model of type 2 diabetes mellitus,and to observe the therapeutic effect of Chinese medicine of Shutangbao Oral Liquid on the model′s blood glucose level,blood lipid level and urea content.Methods Thirty-five Kunming mice were fed high-glucose and high-fat diet for four weeks,and then were injected with streptozotocin(STZ) by intraperitoneal approach to establish the mouse model of type 2 diabetes mellitus.The 35 mice were divided into three group randomly,model group,Shutangbao group and metformin group.Another 10 normal mice as control group.Shutangbao group was treated with Shutangbao Oral Liquid and metformin group with metformin by gavage,normal group and model group were treated with distilled water by gavage.The volumes of drinking water of the mice in each group were observed and recorded,and their blood glucose levels,total cholesterol(TC),triglyceride(TG) and urea content were detected before and after administration.Results The volumes of drinking water per day of the mice in Shutangbao group,and metformin group were less than those of control group,and model group after administration(P<0.01);And the blood glucose levels and TG content of the mice in Shutangbao group,and metformin group were lower than those of model group(P<0.01);Hypoglycemic rates were 42.50% and 35.09%,respectively.Conclusion Shutangbao Oral Liquid can significantly lower the blood glucose level,TG content and the volume of drinking water of mouse with type 2 diabetes mellitus.
Objective To establish a mouse model of type 2 diabetes mellitus,and to observe the therapeutic effect of Chinese medicine of Shutangbao Oral Liquid on the model′s blood glucose level,blood lipid level and urea content.Methods Thirty-five Kunming mice were fed high-glucose and high-fat diet for four weeks,and then were injected with streptozotocin(STZ) by intraperitoneal approach to establish the mouse model of type 2 diabetes mellitus.The 35 mice were divided into three group randomly,model group,Shutangbao group and metformin group.Another 10 normal mice as control group.Shutangbao group was treated with Shutangbao Oral Liquid and metformin group with metformin by gavage,normal group and model group were treated with distilled water by gavage.The volumes of drinking water of the mice in each group were observed and recorded,and their blood glucose levels,total cholesterol(TC),triglyceride(TG) and urea content were detected before and after administration.Results The volumes of drinking water per day of the mice in Shutangbao group,and metformin group were less than those of control group,and model group after administration(P<0.01);And the blood glucose levels and TG content of the mice in Shutangbao group,and metformin group were lower than those of model group(P<0.01);Hypoglycemic rates were 42.50% and 35.09%,respectively.Conclusion Shutangbao Oral Liquid can significantly lower the blood glucose level,TG content and the volume of drinking water of mouse with type 2 diabetes mellitus.
引文
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[10]郭洁文,潘竞锵.荔枝和荔枝核的化学成分、生物活性及药理作用研究[J].中国新药杂志,2006,15(8):585-588.
[11]楼忠明,田菊霞,王文香,等.荔枝核总皂苷提取物对糖尿病小鼠的降糖疗效观察[J].浙江医学,2007,29(6):548-550.
[2]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2010年版)[J].中国糖尿病杂志,2012,20(1):1-37.
[3]李朝敢,张树球,王三艳,等.荔枝核提取液对四氧嘧啶糖尿病模型小鼠血生化指标的干预实验[J].中国临床康复,2006,10(31):61-63.
[4]陈莉,黄日越,钟文晴,等.荔枝核提取液治疗对小鼠糖尿病模型生化指标的干预作用[J].当代医学(学术版),2008,2(3):27-29.
[5]首都医学院主编.医用生物化学实验[M].天津:天津科学技术出版社,1987:92.
[6]周敏,柴可夫.实验性2型糖尿病动物模型研究及其进展[J].浙江中医学院学报,2001,25(5):79-80.
[7]李爱卿,王志慧,赵跃斌.高糖高脂饲料诱导2型糖尿病大鼠模型[J].临床医药实践杂志,2005,14(2):130-131.
[8]Cunha JM,Funez MI,Cunha FQ,et al.Streptozotocin-inducedmechanical hypernociception is not dependent on hyperglycemia[J].Braz J Med Biol Res,2009,42(2):197-206.
[9]郭洁文,潘竞锵,邱光清,等.荔枝核增强2型糖尿病胰岛素抵抗大鼠胰岛素敏感性作用[J].中国新药杂志,2003,12(7):526-528.
[10]郭洁文,潘竞锵.荔枝和荔枝核的化学成分、生物活性及药理作用研究[J].中国新药杂志,2006,15(8):585-588.
[11]楼忠明,田菊霞,王文香,等.荔枝核总皂苷提取物对糖尿病小鼠的降糖疗效观察[J].浙江医学,2007,29(6):548-550.