藏药绿萝花对高血糖小鼠模型的治疗试验
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摘要
研究藏药绿萝花对高血糖动物模型的治疗作用,为临床合理用药和进一步开发利用提供科学依据。采用饲喂高糖高脂饲料和注射链脲佐菌素(STZ)建立ICR小鼠高血糖动物模型,将实验动物分成对照组、模型组、二甲双胍(250mg/kg)组及绿萝花提取物组(剂量分别为50、25、12.5g/kg),分别在造模试验的第14天、第21天、第28天,以及治疗试验的第14天、第21天、第28天,以空腹血清血糖含量、血清胰岛素含量为检测指标,研究造模因素和不同剂量绿萝花对血糖的影响。连续饲喂高糖高脂饲料,结合STZ注射,造模小鼠表现为体重下降、血糖升高、胰岛素含量下降;表明3个剂量绿萝花组均具有降血糖的作用,12.5g/kg剂量组连续服用绿萝花14d后降血糖作用明显,50g/kg剂量组连续口服绿萝花7d~14d,显著提高成模小鼠血清胰岛素含量。连续饲喂高糖高脂饲料(配方:蔗糖∶猪油∶奶粉∶鸡蛋∶猪胆酸盐∶普通饲料=30∶20∶4∶2∶0.05∶63)14d~28d,结合STZ注射的造模方法成功获得了Ⅱ型糖尿病动物模型;绿萝花通过降低成模小鼠血糖含量、升高成模小鼠血清胰岛素含量而对Ⅱ型糖尿病动物模型具有治疗作用。
To study the therapeutic effect of S.aureus on hyperglycemia animal model and provide experimental bases for reasonable clinical medication and development,the hyperglycemia mouse model was made by feeding high sugar and high fat feed to ICR mice.The mice were divided into six groups:control group,model group,melbine group(250mg/kg)and 50,25,12.5g/kg doses of S.aureus extract groups.To study building factors and the role of S.aureusin hpyerglycemia,the fasting blood glucose and serum insulin,insulin resistance index were used in the 14 th day,the 21 st day and the 28 th day of model preparation and treatment experiment.The result of model preparation showed that ICR mouse body weight decreased,blood glucose increased,insulin levels droped and insulin resistance index increased through feeding high glucose and high fat feed combined with injecting STZ.The result of treatment experiment showed that blood sugar level was regulated by the three doses.Low dose group had obvious effect on hyperglycemia after oral administration of S.aureus for forteen days.High dose group had obvious effect on enhancing insulin level after oral administration of S.aureus from the 7st day to the 14 st day.The three doses had no obvious effect on insulin resistance index.The hyperglycemia mouse model was made successfully by feeding high glucose and high fat feed for 14 to 28days(formula:sucrose∶lard∶milk powder∶egg∶pig cholate∶standard diet=30∶20∶4∶2∶0.05∶63)and combined with injecting STZ;The hyperglycemia mouse model could be cured through regulating blood sugar level,increasing serum insulin and reducing insulin resistance index.
To study the therapeutic effect of S.aureus on hyperglycemia animal model and provide experimental bases for reasonable clinical medication and development,the hyperglycemia mouse model was made by feeding high sugar and high fat feed to ICR mice.The mice were divided into six groups:control group,model group,melbine group(250mg/kg)and 50,25,12.5g/kg doses of S.aureus extract groups.To study building factors and the role of S.aureusin hpyerglycemia,the fasting blood glucose and serum insulin,insulin resistance index were used in the 14 th day,the 21 st day and the 28 th day of model preparation and treatment experiment.The result of model preparation showed that ICR mouse body weight decreased,blood glucose increased,insulin levels droped and insulin resistance index increased through feeding high glucose and high fat feed combined with injecting STZ.The result of treatment experiment showed that blood sugar level was regulated by the three doses.Low dose group had obvious effect on hyperglycemia after oral administration of S.aureus for forteen days.High dose group had obvious effect on enhancing insulin level after oral administration of S.aureus from the 7st day to the 14 st day.The three doses had no obvious effect on insulin resistance index.The hyperglycemia mouse model was made successfully by feeding high glucose and high fat feed for 14 to 28days(formula:sucrose∶lard∶milk powder∶egg∶pig cholate∶standard diet=30∶20∶4∶2∶0.05∶63)and combined with injecting STZ;The hyperglycemia mouse model could be cured through regulating blood sugar level,increasing serum insulin and reducing insulin resistance index.
引文
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[14]于淑池,苏涛,杨建民,等.安吉白茶多糖对实验性糖尿病小鼠的降血糖作用研究[J].茶叶科学,2010,30(3):223-228.
[15]李强,丁实,周剑宇,等.高脂饮食合并不同剂量STZ建立小鼠2型糖尿病模型[J].承德医学学院学报,2013,30(1):5-7.
[16]李韬,陶吉英,黄广燕,等.用链佐霉素(STZ)建立2型糖尿病小鼠模型及中药治疗效果的研究[J].实用糖尿病杂志,2011,8(1):18-20.
[17]朱晓莹,黄广燕,黎秋萍,等.舒糖宝口服液治疗2型糖尿病小鼠模型的效果观察[J].广西医学,2013,35(1):35-37.
[18]郭啸华,刘志红,李恒,等.实验性2型糖尿病大鼠模型的建立[J].肾脏病与透析肾移植杂志,2000,9(4):351-355
[19]杨娜,孟宪丽,董光新,等.链脲佐菌素加膳食诱导的小鼠2型糖尿病模型研究[J].中药药理与临床,2007,23(1):74-76.
[20]牛屹东,梁蜀龙.C57BL/6J小鼠在构建2型糖尿病模型中体重[J].动物学研究,2007,28(5):507-510.
[21]汤晓丽,邓立彬,李桂林,等.2型糖尿病早期大鼠外周神经节基因表达谱分析[J].遗传,2012(2):198-257.
[22]丛朋地,钟进义,张静,等.补镁对2型糖尿病大鼠胰岛素受体表达水平的影响[J].卫生研究,2012(2):264-267.
[23]陈小明,黄丹丹,李自成.新西兰兔糖尿病肾病模型的建立方法[J].中国病理生理杂志,2012(4):765-768.
[24]孙兆峰,王利,夏作理,等.2型糖尿病动物模型研究概要[J].中国微循环,2008(3):187-189.
[25]周东海,王海峰,杜长海,等.怀柔区217例糖尿病患者中医辨证分型与[J].北京中医药,2009,28(1),39-40.
[26]赵进喜,王世东,李靖,等.糖尿病中医辨证与疗效评价标准及其研究[J].世界中医药,2013(8):504-507.
[27]施红,王麒又,辛金钟,等.2型糖尿病大鼠模型中医证型演变的实验研究[J].中医杂志,2011,52(5):408-412.
[28]黄松.糖尿病动物模型研究现状及进展[J].广西医学,2002,24(1):46-48.
[29]陈嘉,王萧,张永斌,等.两种外科手术对2型糖尿病大鼠胰腺SUR1表达的影响[J].动物医学进展,2013,34(1):24-28.
[30]黄福开.藏医养生图说[M]北京:人民卫生出版社,2006:235.
[31]张晶晶,孔凡青,李嘉欣,等.棕榈酸对胰岛MIN6细胞胰岛素成熟的影响[J].动物医学进展,2013,34(2):44-48.
[2]黄福开.藏医养生图说[M].北京:人民卫生出版社,2006:235.
[3]曾艺玲,李莉.藏药绿萝花超临界萃取工艺优化及降血糖作用研究[J].中国民族民间医药,2008(10):7-9.
[4]竺琴,张焜,林志云,等.西藏绿萝花提取物对α-葡萄糖苷酶的抑制及抗氧化作用[J].中药材,2009,32(1):89-92.
[5]韩亮,郭晓玲,冯毅凡,等.藏药绿萝花挥发性成分GC-MS分析[J].中国民族民间医药,2009,18(9):148.
[6]陈家鹏.西藏绿萝花中黄酮和还原糖含量测定研究[J].中国民族民间医药,2010,19(13):2-3.
[7]李文茂,石玉平,刘春缨,等.藏药绿萝花中总黄酮含量的提取工艺研究[J].北方园艺,2013(5):144-146.
[8]魏永生,郑敏燕,耿薇,等.西藏绿萝花中矿质元素的分析测定[J].林产化学与工业,2013,33(1):103-106.
[9]太志刚.四种花卉的化学成分及其抗氧化活性研究[D].云南昆明:云南大学,2011.
[10]钟晓明,黄真,毛庆秋.正交试验法优选北沙参欧前胡素的提取工艺[J].中成药,2006,28(5):749-750.
[11]张素萍,胡能.中药提取生产工艺优化的实验设计[J].贵州科技工程职业学院学报,2008,3(3):3-5.
[12]程旸,许立,许波华,等.四妙颗粒对湿热困脾型糖尿病模型小鼠血糖及糖耐量的影响[J].中南药学,2011,9(4):245-248.
[13]王松笛,肖菲菲,殷莉,等.毫州丹皮水提物对2型糖尿病小鼠血糖、血脂及抗氧化能力的作用研究[J].中国药师,2011,14(8):1082-1085.
[14]于淑池,苏涛,杨建民,等.安吉白茶多糖对实验性糖尿病小鼠的降血糖作用研究[J].茶叶科学,2010,30(3):223-228.
[15]李强,丁实,周剑宇,等.高脂饮食合并不同剂量STZ建立小鼠2型糖尿病模型[J].承德医学学院学报,2013,30(1):5-7.
[16]李韬,陶吉英,黄广燕,等.用链佐霉素(STZ)建立2型糖尿病小鼠模型及中药治疗效果的研究[J].实用糖尿病杂志,2011,8(1):18-20.
[17]朱晓莹,黄广燕,黎秋萍,等.舒糖宝口服液治疗2型糖尿病小鼠模型的效果观察[J].广西医学,2013,35(1):35-37.
[18]郭啸华,刘志红,李恒,等.实验性2型糖尿病大鼠模型的建立[J].肾脏病与透析肾移植杂志,2000,9(4):351-355
[19]杨娜,孟宪丽,董光新,等.链脲佐菌素加膳食诱导的小鼠2型糖尿病模型研究[J].中药药理与临床,2007,23(1):74-76.
[20]牛屹东,梁蜀龙.C57BL/6J小鼠在构建2型糖尿病模型中体重[J].动物学研究,2007,28(5):507-510.
[21]汤晓丽,邓立彬,李桂林,等.2型糖尿病早期大鼠外周神经节基因表达谱分析[J].遗传,2012(2):198-257.
[22]丛朋地,钟进义,张静,等.补镁对2型糖尿病大鼠胰岛素受体表达水平的影响[J].卫生研究,2012(2):264-267.
[23]陈小明,黄丹丹,李自成.新西兰兔糖尿病肾病模型的建立方法[J].中国病理生理杂志,2012(4):765-768.
[24]孙兆峰,王利,夏作理,等.2型糖尿病动物模型研究概要[J].中国微循环,2008(3):187-189.
[25]周东海,王海峰,杜长海,等.怀柔区217例糖尿病患者中医辨证分型与[J].北京中医药,2009,28(1),39-40.
[26]赵进喜,王世东,李靖,等.糖尿病中医辨证与疗效评价标准及其研究[J].世界中医药,2013(8):504-507.
[27]施红,王麒又,辛金钟,等.2型糖尿病大鼠模型中医证型演变的实验研究[J].中医杂志,2011,52(5):408-412.
[28]黄松.糖尿病动物模型研究现状及进展[J].广西医学,2002,24(1):46-48.
[29]陈嘉,王萧,张永斌,等.两种外科手术对2型糖尿病大鼠胰腺SUR1表达的影响[J].动物医学进展,2013,34(1):24-28.
[30]黄福开.藏医养生图说[M]北京:人民卫生出版社,2006:235.
[31]张晶晶,孔凡青,李嘉欣,等.棕榈酸对胰岛MIN6细胞胰岛素成熟的影响[J].动物医学进展,2013,34(2):44-48.