西北高海拔地区放养偶蹄类动物肠道微生物多样性的宏基因组比较研究
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摘要
[目的]分析西北高海拔地区偶蹄类动物肠道的微生物多样性。[方法]从动物粪便样品中提取全部微生物的DNA,利用宏基因组学方法,通过高通量测序及生物信息学分析对肠道微生物多样性及群落功能进行比较。[结果]在相似的地理环境下,不同物种偶蹄类动物的肠道微生物多样性存在高度的相似性,暗示着生存环境对动物肠道微生物的多样性发挥着重要影响。[结论]该研究初步阐明了西北地区高海拔环境下不同种类动物的肠道微生物多样性,为进一步了解高原环境中微生物的构成提供了重要参考。
[Objective] To analyze the intestinal microbial diversity of cloven-hoofed animals in high elevation area of northwest China. [Method] The total DNA were extracted from feces samples of test animals, and the intestinal microbial diversity and community function were analyzed by using metagenomic analysis with high throughput sequencing and bioinformatics analysis.[Result]Different species of cloven-hoofed animals had high similarity in a similar geographical environment, which suggested the environment played an important role in the microbial diversity of animal intestines. [Conclusion] The intestinal microbial diversity of Chinese cloven-hoofed animals from the high altitude areas of northwest China were preliminary illuminated, which provided important references for further understanding the composition of microorganisms in the plateau environment.
[Objective] To analyze the intestinal microbial diversity of cloven-hoofed animals in high elevation area of northwest China. [Method] The total DNA were extracted from feces samples of test animals, and the intestinal microbial diversity and community function were analyzed by using metagenomic analysis with high throughput sequencing and bioinformatics analysis.[Result]Different species of cloven-hoofed animals had high similarity in a similar geographical environment, which suggested the environment played an important role in the microbial diversity of animal intestines. [Conclusion] The intestinal microbial diversity of Chinese cloven-hoofed animals from the high altitude areas of northwest China were preliminary illuminated, which provided important references for further understanding the composition of microorganisms in the plateau environment.
引文
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[1] 樊程,李双江,李成磊,等.大熊猫肠道纤维素分解菌的分离鉴定及产酶性质[J].微生物学报,2012,52(9):1113-1121.
[2] WANG W,ZHENG S S,SHARSHOV K,et al.Metagenomic profiling of gut microbial communities in both wild and artificially reared Bar-headed goose(Anser indicus)[J].Microbiology open,2017,6(2):1-9.
[3] 兰阿峰,杨曼,郭素芬,等.免培养法对大鲵肠道微生物多样性的研究[J].微生物学通报,2014,41(7):1342-1349.
[4] 刘莉,王中康,俞和韦,等.贡嘎蝠蛾幼虫肠道细菌多样性分析[J].微生物学报,2008,48(5):616-622.
[5] 杨曼,兰阿峰,郭素芬,等.免培养法研究野生川金丝猴肠道内生细菌多样性[J].微生物学通报,2014,41(8):1605-1612.
[6] 白秀娟,刘诚刚,杜智恒,等.PCR-DGGE技术分析断奶仔兔肠道微生物菌群结构及多样性[J].东北农业大学学报,2012,43(9):64-69.
[7] 许宇静,张煜坤,沈雪梅,等.采用Illumina MiSeq测序技术分析断奶幼兔肠道微生物群落的多样性[J].动物营养学报,2015,27(9):2793-2802.
[8] BHATT V D,DANDE S S,PATIL N V,et al.Molecular analysis of the bacterial microbiome in the forestomach fluid from the dromedary camel(Camelus dromedarius)[J].Mol Biol Rep,2013,40(4):3363-3371.