摘要
在线形离子阱中,通过质量选择离子的碰撞诱导解离实现串级质谱分析。在现有的实验过程中,离子的碰撞诱导解离是通过在一对电极上加载偶极激发信号实现离子共振激发和碰撞解离,即单向离子激发。本研究基于数字离子阱技术,在线形离子阱的二对电极上加载离子激发电压,实现离子的双向共振激发,以提高离子的激发能量和碰撞解离效率。理论模拟计算和实验结果表明,在不同的q值和不同的激发频率下,双向激发获得更高的碰撞解离效率。此外,在q值为0.352、激发电压为0.5 V_(0-p)、激发频率为107.93 kHz、解离时间为5 ms时,双向激发解离可以获得亮氨酸脑啡肽(m/z=556)更多的低质量数碎片离子峰,降低了离子阱质谱的低质量截止效应,显著提高了离子阱质谱的串级质谱分析性能。
Tandem mass spectrometric analysis is one primary function of ion trap mass analyzer. In linear ion trap, this function is conducted by collision induced dissociation(CID), which is realized via applying dipolar excitation voltage on one couple of electrodes, so the CID method is also known as unidirectional ion excitation. In this work, bidirectional resonance excitation method was introduced. By applying dipolar excitation voltage on two couples of electrodes, the ion excitation energy and associated dissociation efficiency of this method were increased. The simulation and experimental results indicated that based on this method, higher ion dissociation efficiency was obtained with different q values and excitation frequencies. Besides, more small fragments could be detected in tandem mass analysis of leucine enkephalin via bidirectional resonance excitation with q=0.352, V=0.5 V_(0-p), ω=107.93 kHz and t=5 ms, suggesting that the method could effectively decrease low mass cut-off effect and strength ability of ion trap on tandem mass analysis.
引文
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