马铃薯块茎发育期主要植物激素的动态变化
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摘要
为了明晰几种主要的植物激素在马铃薯块茎形成中的作用,本研究采用LC-MS/MS法测定了马铃品种‘PB06’及品种‘会-2’块茎发育不同时期叶片、匍匐茎/块茎组织中植物激素ABA、GA3、JA和IAA含量的动态变化。结果显示,随着块茎的发育,匍匐茎/块茎中ABA及JA含量增加;叶片中ABA含量逐渐增加,JA含量先上升后下降,两个品种变化趋势相同。GA3的积累随着匍匐茎的膨大而下降;IAA含量峰值出现在匍匐茎膨大时,并且在之后匍匐茎的膨大中仍然维持高含量水平。表明,IAA及GA3对匍匐茎的伸长是必要的,ABA、JA是诱导块茎形成的正调控因子,GA3为负调控因子。该研究结果对开展植物激素调控块茎形成机理的研究具有重要的支撑作用。
To clarify the roles of key plant hormones in the formation of potato tubers, we followed the dynamic changes of ABA, GA3, JA and IAA content during the tuber four consecutive developmental stages of Solanum tuberosum L. 'Hui 2' and 'PB06' by LC-MS/MS. The results showed that the contents of ABA and JA in leaves and stolon/tuber and accumulation of JA in stolon/tuber increased throughout the whole tuber development, while JA amounts increased from tuberization induction to tuber initiation and decreased from tuber grow to ripe stage. The accumulation of GA3 decreased with the swelling of stolons. IAA amounts showed an increase-decrease tendency with a peak at tuber initiation stage. The results showed IAA and GA3 are essential for elongation of stolon. In addition, ABA and JA are positive regulatory factors for tuberization, while GA is a dominant negative regulator that inhibits tuber formation. The results play a supporting role in the research on mechanism of phytohormones involved tuberization.
To clarify the roles of key plant hormones in the formation of potato tubers, we followed the dynamic changes of ABA, GA3, JA and IAA content during the tuber four consecutive developmental stages of Solanum tuberosum L. 'Hui 2' and 'PB06' by LC-MS/MS. The results showed that the contents of ABA and JA in leaves and stolon/tuber and accumulation of JA in stolon/tuber increased throughout the whole tuber development, while JA amounts increased from tuberization induction to tuber initiation and decreased from tuber grow to ripe stage. The accumulation of GA3 decreased with the swelling of stolons. IAA amounts showed an increase-decrease tendency with a peak at tuber initiation stage. The results showed IAA and GA3 are essential for elongation of stolon. In addition, ABA and JA are positive regulatory factors for tuberization, while GA is a dominant negative regulator that inhibits tuber formation. The results play a supporting role in the research on mechanism of phytohormones involved tuberization.
引文
Abdala G.,Castro G.,Miersch O.,and Pearce D.,2002,Changes in jasmonate and gibberellin levels during development of potato plants(Solanum tuberosum),Plant Growth Regul.,36(2):121-126
Aksenova N.P.,Konstantinova T.N.,Golyanovskaya S.A.,Sergeeva L.I.,and Romanov G.A.,2012,Hormonal regulation of tuber formation in potato plants.Russ.J.Plant Physl.,59(4):491-508
Cai R.X.,Wu Z.G.,Zhou J.X.,and Liu Z.P.,2016,Popularization of potato as staple food in China:a case study of Zhejiang province,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(2):467-471(蔡仁祥,吴早贵,周建祥,刘振鹏,2016,中国马铃薯主食化---浙江省的发展对策,基因组学与应用生物学,35(2):467-471)
Dick V.,and Struik P.C.,1989,An integrated view of the hormonal regulation of the tuber formation in potato(Solanum tuberosum L.),Physiol.Plantarum,75(4):525-531
Dutt S.,Manjul A.S.,Raigond P.,Singh B.,Siddappa S.,Bhardwaj V.,Kawar P.G.,Patil V.U.,and Kardile H.B.,2017,Key players associated with tuberization in potato:potential candidates for genetic engineering,Crit.Rev.Biotechnol.,37(7):942-957
Efstathios R.,Bjorn K.,Marian O.,Wouter K.,Bouwmeester H.J.,Visser R.G.F.,and Bachem C.W.B.,2012,The effects of auxin and strigolactones on tuber initiation and stolon architecture in potato,J.Exp.Bot.,63(12):4539-4547
Ewing E.E.,1987,The role of hormones in potato(Solanum tuberosum L.)tuberization,In:Davies P.J.(ed.),Plant hormones and their role in plant growth and development,Martinus Nijhoff Publishers,Boston,USA,pp.515-538
Ewing E.E.,and Struik P.C.,1992,Tuber formation in potato:induction,initiation,and growth,Hortic.Rev.,14(1992):189-198
Faivre-Rampant O.,Cardle L.,Marshall D.,Viola R.,and Taylor M.A.,2004,Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene,J.Exp.Bot.,55(397):613-622
Hannapel D.J.,Chen H.,Rosin F.M.,Banerjee A.K.,and Davies P.J.,2004,Molecular controls of tuberization,Am.J.Potato Res.,81(4):263-274
Jackson S.D.,Prat S.,and Eacute.,1996,Control of tuberisation in potato by gibberellins and phytochrome B,Physiol.Plantarum,98(2):407-412
Jiang Y.,Liang G.,Yang S.,and Yu D.,2014,Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid and auxin-mediated signaling in jasmonic acid-induced leaf senescence,Plant Cell,26(1):230-245
Kloosterman B.,De K.D.,Griffiths R.,Flinn B.,Steuernagel B.,Scholz U.,Sonnewald S.,Sonnewald U.,Bryan G.J.,and Prat S.,2008,Genes driving potato tuber initiation and growth:identification based on transcriptional changes using the POCI array,Funct.Integr.Genomics,8(4):329-340
Kloosterman B.,Navarro C.,Bijsterbosch G.,Lange T.,Prat S.,Visser R.G.,and Bachem C.,2007,StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development,Plant J.,52(2):362-373
Krauss A.,and Marschner H.,1982,Influence of nitrogen nutrition,daylength and temperature on contents of gibberellic and abscisic acid and on tuberization in potato plants,Potato Res.,25(1):13-21
Langille A.R.,1978,Physiology of tuberization in Solanum tuberosum L.:cis-zeatin riboside in the potato plant:its identification and changes in endogenous levels as influenced by temperature and photoperiod,Plant Physiol.,62(3):438-442
Liu M.Y.,Meng M.L.,Men F.Y.,and Mao X.F.,1996,The change of intrinsic hormones in the growth period of potato,Zhongguo Malingshu(Chinese Potato Journal),10(4):197-202(刘梦芸,蒙美莲,门福义,毛雪飞,1996,马铃薯生育期间内源激素的变化,中国马铃薯,10(4):197-202)
Mu觡iz G.M.N.,Stritzler M.,and Capiati D.A.,2014,Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation,Planta,239(3):615-631
Pasare S.A.,Ducreux L.J.M.,Morris W.L.,Campbell R.,Sharma S.K.,Roumeliotis E.,Kohlen W.,Krol S.,Bramley P.M.,and Roberts A.G.,2013,The role of the potato(Solanum tuberosum)CCD8 gene in stolon and tuber development,New Phytol.,198(4):1108-1120
Pelacho A.M.,and Mingo-Castel A.M.,1991,Jasmonic acid induces tuberization of potato stolons cultured in vitro,Plant Physiol.,97(3):1253-1255
Peng Q.,Wang H.Q.,Tong J.H.,Kabir M.H.,Huang Z.G.,and Xiao L.T.,2013,Effects of indole-3-acetic acid and auxin transport inhibitor on auxin distribution and development of peanut at pegging stage,Sci.Hortic.,162(3):76-81
Railton I.D.,and Wareing P.F.,1973,Effects of abscisic acid on the levels of endogenous gibberellin-like substances in Solanum andigena,Planta,112(1):65-69
Roumeliotis E.,Visser R.G.,and Bachem C.W.,2012,Acrosstalk of auxin and GA during tuber development,Plant Signal.Behav.,7(10):1360-1363
Shu K.,Zhou W.,and Yang W.,2017,APETALA 2-domain-containing transcription factors:focusing on abscisic acid and gibberellins antagonism,New Phytol.,217(3):977-983
Tao G.Q.,Letham D.S.,Yong J.W.H.,Zhang K.R.,John P.C.L.,Schwartz O.,Wong S.C.,and Farquhar G.D.,2010,Promotion of shoot development and tuberisation in potato by expression of a chimaeric cytokinin synthesis gene at normal and elevated CO2levels,Funct.Plant Biol.,37(1):43-54
Xu X.,1998,The role of gibberellin,abscisic acid,and sucrose in the regulation of potato tuber formation in vitro,Plant Physiol.,117(2):575-584
Zhou L.J.,Xiao L.T.,and Xue H.W.,2017,Dynamic cytology and transcriptional regulation of rice lamina joint development,Plant Physiol.,174(3):1728-1746
Aksenova N.P.,Konstantinova T.N.,Golyanovskaya S.A.,Sergeeva L.I.,and Romanov G.A.,2012,Hormonal regulation of tuber formation in potato plants.Russ.J.Plant Physl.,59(4):491-508
Cai R.X.,Wu Z.G.,Zhou J.X.,and Liu Z.P.,2016,Popularization of potato as staple food in China:a case study of Zhejiang province,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(2):467-471(蔡仁祥,吴早贵,周建祥,刘振鹏,2016,中国马铃薯主食化---浙江省的发展对策,基因组学与应用生物学,35(2):467-471)
Dick V.,and Struik P.C.,1989,An integrated view of the hormonal regulation of the tuber formation in potato(Solanum tuberosum L.),Physiol.Plantarum,75(4):525-531
Dutt S.,Manjul A.S.,Raigond P.,Singh B.,Siddappa S.,Bhardwaj V.,Kawar P.G.,Patil V.U.,and Kardile H.B.,2017,Key players associated with tuberization in potato:potential candidates for genetic engineering,Crit.Rev.Biotechnol.,37(7):942-957
Efstathios R.,Bjorn K.,Marian O.,Wouter K.,Bouwmeester H.J.,Visser R.G.F.,and Bachem C.W.B.,2012,The effects of auxin and strigolactones on tuber initiation and stolon architecture in potato,J.Exp.Bot.,63(12):4539-4547
Ewing E.E.,1987,The role of hormones in potato(Solanum tuberosum L.)tuberization,In:Davies P.J.(ed.),Plant hormones and their role in plant growth and development,Martinus Nijhoff Publishers,Boston,USA,pp.515-538
Ewing E.E.,and Struik P.C.,1992,Tuber formation in potato:induction,initiation,and growth,Hortic.Rev.,14(1992):189-198
Faivre-Rampant O.,Cardle L.,Marshall D.,Viola R.,and Taylor M.A.,2004,Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene,J.Exp.Bot.,55(397):613-622
Hannapel D.J.,Chen H.,Rosin F.M.,Banerjee A.K.,and Davies P.J.,2004,Molecular controls of tuberization,Am.J.Potato Res.,81(4):263-274
Jackson S.D.,Prat S.,and Eacute.,1996,Control of tuberisation in potato by gibberellins and phytochrome B,Physiol.Plantarum,98(2):407-412
Jiang Y.,Liang G.,Yang S.,and Yu D.,2014,Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid and auxin-mediated signaling in jasmonic acid-induced leaf senescence,Plant Cell,26(1):230-245
Kloosterman B.,De K.D.,Griffiths R.,Flinn B.,Steuernagel B.,Scholz U.,Sonnewald S.,Sonnewald U.,Bryan G.J.,and Prat S.,2008,Genes driving potato tuber initiation and growth:identification based on transcriptional changes using the POCI array,Funct.Integr.Genomics,8(4):329-340
Kloosterman B.,Navarro C.,Bijsterbosch G.,Lange T.,Prat S.,Visser R.G.,and Bachem C.,2007,StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development,Plant J.,52(2):362-373
Krauss A.,and Marschner H.,1982,Influence of nitrogen nutrition,daylength and temperature on contents of gibberellic and abscisic acid and on tuberization in potato plants,Potato Res.,25(1):13-21
Langille A.R.,1978,Physiology of tuberization in Solanum tuberosum L.:cis-zeatin riboside in the potato plant:its identification and changes in endogenous levels as influenced by temperature and photoperiod,Plant Physiol.,62(3):438-442
Liu M.Y.,Meng M.L.,Men F.Y.,and Mao X.F.,1996,The change of intrinsic hormones in the growth period of potato,Zhongguo Malingshu(Chinese Potato Journal),10(4):197-202(刘梦芸,蒙美莲,门福义,毛雪飞,1996,马铃薯生育期间内源激素的变化,中国马铃薯,10(4):197-202)
Mu觡iz G.M.N.,Stritzler M.,and Capiati D.A.,2014,Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation,Planta,239(3):615-631
Pasare S.A.,Ducreux L.J.M.,Morris W.L.,Campbell R.,Sharma S.K.,Roumeliotis E.,Kohlen W.,Krol S.,Bramley P.M.,and Roberts A.G.,2013,The role of the potato(Solanum tuberosum)CCD8 gene in stolon and tuber development,New Phytol.,198(4):1108-1120
Pelacho A.M.,and Mingo-Castel A.M.,1991,Jasmonic acid induces tuberization of potato stolons cultured in vitro,Plant Physiol.,97(3):1253-1255
Peng Q.,Wang H.Q.,Tong J.H.,Kabir M.H.,Huang Z.G.,and Xiao L.T.,2013,Effects of indole-3-acetic acid and auxin transport inhibitor on auxin distribution and development of peanut at pegging stage,Sci.Hortic.,162(3):76-81
Railton I.D.,and Wareing P.F.,1973,Effects of abscisic acid on the levels of endogenous gibberellin-like substances in Solanum andigena,Planta,112(1):65-69
Roumeliotis E.,Visser R.G.,and Bachem C.W.,2012,Acrosstalk of auxin and GA during tuber development,Plant Signal.Behav.,7(10):1360-1363
Shu K.,Zhou W.,and Yang W.,2017,APETALA 2-domain-containing transcription factors:focusing on abscisic acid and gibberellins antagonism,New Phytol.,217(3):977-983
Tao G.Q.,Letham D.S.,Yong J.W.H.,Zhang K.R.,John P.C.L.,Schwartz O.,Wong S.C.,and Farquhar G.D.,2010,Promotion of shoot development and tuberisation in potato by expression of a chimaeric cytokinin synthesis gene at normal and elevated CO2levels,Funct.Plant Biol.,37(1):43-54
Xu X.,1998,The role of gibberellin,abscisic acid,and sucrose in the regulation of potato tuber formation in vitro,Plant Physiol.,117(2):575-584
Zhou L.J.,Xiao L.T.,and Xue H.W.,2017,Dynamic cytology and transcriptional regulation of rice lamina joint development,Plant Physiol.,174(3):1728-1746