β-catenin与Fascin-1在结肠癌中的表达及与肿瘤病情的相关性
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摘要
目的探讨结肠癌组织中的β连环蛋白(β-catenin)、Fascin-1蛋白表达变化及临床意义。方法回顾性选取2015年1月至2017年6月遂宁市中心医院收集的术后结肠癌组织标本(癌组织) 60例、30例结肠癌癌旁组织(距离病灶边缘5 cm以内的正常结肠组织,对照组)。采用免疫组化染色检测β-catenin与Fascin-1蛋白的表达,并分析二者的相关性及其临床意义。结果癌组织的Fascin-1蛋白、β-catenin蛋白阳性表达率分别为51. 67%、78. 33%均高于对照组的10. 00%、16. 67%,差异具有统计学意义(P <0. 05);癌组织的Fascin-1蛋白与β-catenin蛋白阳性表达呈正相关关系(r=0. 382,P <0. 05);癌组织的Fascin-1蛋白阳性表达与TNM分期、发生淋巴结转移有关(P <0. 05);癌组织的Fascin-1蛋白阳性表达与TNM分期、肿瘤分化程度、侵及浆膜、发生淋巴结转移有关(P <0. 05)。结论β-catenin与Fascin-1蛋白在结肠癌组织中高表达,二者呈正相关表达,并且与肿瘤TNM分期及发生淋巴结转移有关。
Objective To investigate the relationship between the expression and relationship of β-catenin and fascin-1 protein in colon cancer tissues,and the relationship with tumor development. Methods 60 cases of colon cancer tissues and 30 cases of colon cancer adjacent tissues( normal colon tissue within 5 cm from the edge of the lesion,control group) were collected in our hospital from January 2015 to June 2017 and underwent immunohistochemical staining. The expression of β-catenin and fascin-1 protein was detected,and their correlation and clinical significance were analyzed. Results The positive expression rate of fascin-1 protein and β-catenin protein in cancer tissues were 51. 67% and78. 33%,higher than in control group( 10. 00% and 16. 67%)( P < 0. 05). Positive correlation existed between fascin-1 and β-catenin protein expression( r = 0. 382,P < 0. 05). Fascin-1 expression in cancer tissue was related to TNM stages,tumor differentiation,serosa invasion and lymph node metastasis( P < 0. 05). Conclusion β-catenin and fascin-1 proteins are highly expressed in colon cancer tissues,and they are positively correlated with each other,and have close relationship with tumor development.
Objective To investigate the relationship between the expression and relationship of β-catenin and fascin-1 protein in colon cancer tissues,and the relationship with tumor development. Methods 60 cases of colon cancer tissues and 30 cases of colon cancer adjacent tissues( normal colon tissue within 5 cm from the edge of the lesion,control group) were collected in our hospital from January 2015 to June 2017 and underwent immunohistochemical staining. The expression of β-catenin and fascin-1 protein was detected,and their correlation and clinical significance were analyzed. Results The positive expression rate of fascin-1 protein and β-catenin protein in cancer tissues were 51. 67% and78. 33%,higher than in control group( 10. 00% and 16. 67%)( P < 0. 05). Positive correlation existed between fascin-1 and β-catenin protein expression( r = 0. 382,P < 0. 05). Fascin-1 expression in cancer tissue was related to TNM stages,tumor differentiation,serosa invasion and lymph node metastasis( P < 0. 05). Conclusion β-catenin and fascin-1 proteins are highly expressed in colon cancer tissues,and they are positively correlated with each other,and have close relationship with tumor development.
引文
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[14]陈旭权,周宏众,张智勇,等. Fascin、β-catenin、E-cadherin在大肠腺瘤癌变过程中的表达及意义[J].中华全科医学,2015,13(5):849-851.
[15]曹国锋,咸华.β-Catenin和Lgr5基因在先天性巨结肠中的表达[J].江苏医药,2014,40(14):1675-1677.
[16]李宏伟,张海,许若飞,等.结直肠腺癌中Galectin-3、Fascin-1和β-catenin蛋白表达与其临床病理特征的关系[J].中国普外基础与临床杂志,2012,19(4):410-415.
[2] Cross-Knorr S,Lu S,Perez K,et al. RKIP phosphorylation and STAT3 activation is inhibited by oxaliplatin and camptothecin and are associated with poor prognosis in stage II colon cancer patients[J]. BMC Cancer,2013,13(1):45-47.
[3]木尔扯尔,文彬,黄一凡,等.β-catenin与wisp-1在结肠癌中的表达及意义[J].现代肿瘤医学,2014,22(2):366-370.
[4]王海波,何朝霞,张欣欣,等. Fascin在结肠癌中表达的临床病理学研究[J].现代生物医学进展,2014,14(20):3880-3883.
[5]王超群,黄必飞,吴正升,等.乳腺癌中Fascin-1蛋白表达及其临床病理意义[J].中华病理学杂志,2014,43(7):451-454.
[6] Sugimoto K,Sakamoto K,Tomiki Y,et al. The validity of predicting prognosis by the lymph node ratio in node-positive colon cancer[J].Dig Surg,2013,30(4-6):368-374.
[7] Ghiringhelli F,Hennequin A,Drouillard A,et al. Epidemiology and prognosis of synchronous and metachronous colon cancer metastases:A French population-based study[J]. Dig Liver Dis,2014,46(9):854-858.
[8] De Sousa E Melo F,Wang X,Jansen M,et al. Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions[J]. Nat Med,2013,19(5):614-618.
[9]郝习,钱丽丽,曹静.β-catenin表达水平与直肠锯齿状癌变的相关性研究[J].临床和实验医学杂志,2016,15(16):1592-1594.
[10]何绍亚,向阳生,蒋婕,等.不同结肠组织中β-catenin和TGF-β1表达与临床病理的关系研究[J].贵州医药,2015,39(9):831-832.
[11]孔凡良,胡丽霞,李成发,等. Fascin-1蛋白在结直肠癌中的表达及其临床意义[J].重庆医学,2016,45(11):1519-1521.
[12]李晟,蔡琳,刘启玲,等.结直肠癌中K-ras基因突变与Fascin-1蛋白表达的相关性[J].临床与实验病理学杂志,2015,31(8):869-872.
[13]宋晓昕. Wnt信号通路中β-catenin、GSK3β及Lef-1在卵巢癌中的表达及其意义[D].石家庄:河北医科大学,2011.
[14]陈旭权,周宏众,张智勇,等. Fascin、β-catenin、E-cadherin在大肠腺瘤癌变过程中的表达及意义[J].中华全科医学,2015,13(5):849-851.
[15]曹国锋,咸华.β-Catenin和Lgr5基因在先天性巨结肠中的表达[J].江苏医药,2014,40(14):1675-1677.
[16]李宏伟,张海,许若飞,等.结直肠腺癌中Galectin-3、Fascin-1和β-catenin蛋白表达与其临床病理特征的关系[J].中国普外基础与临床杂志,2012,19(4):410-415.