烯草酮和镉对芹菜生理生化过程的影响及其毒性机理
|
摘要
通过盆栽试验,评价烯草酮和镉单一污染及复合污染对芹菜生理生化过程的影响,探究复合污染的危害及两者之间是否具有相互作用。对不同处理下芹菜中的SOD、POD、CAT、GSH等酶活性,以及丙二醛、脯氨酸、总酚和镉含量的变化进行研究。结果表明,复合污染相比于单一因素处理,对芹菜有更大的毒害作用,氧化酶系统在受到伤害时,芹菜同时表现出GSH、总酚和脯氨酸含量升高,减轻植株受到的氧化损伤,致使丙二醛的含量有一定的下降。烯草酮和镉复合污染,两者之间存在拮抗作用,烯草酮的施加对于芹菜中镉的富集有一定的抑制作用,且在镉浓度为0.7 mg/kg时,抑制效果明显。
Through pot experiment, an evaluation of the single and combined stress effects of the cadmium and clethodim on the physiological and biochemical process of celery were made. Meanwhile the study explored the interaction between the two combined pollution. The experiment determines the enzyme activities of SOD, POD, CAT, GSH and the content of MDA,proline, total phenols and cadmium content. The results showed that the combined pollution of clethodim and cadmium had more damage to celery, compared to the single factor treatment. The enzymes hurted was one of toxic mechanism of clethodim and cadmium, celery showed the increase of total phenols, proline and GSH, which could alleviate the oxidative damage and decrease the content of MDA. There was a certain antagonism between clethodim and cadmium, Clethodim had certain inhibitory effect on the enrichment of cadmium in celery, when the concentration of cadmium was 0.7 mg/kg, the inhibitory effect was obvious.
Through pot experiment, an evaluation of the single and combined stress effects of the cadmium and clethodim on the physiological and biochemical process of celery were made. Meanwhile the study explored the interaction between the two combined pollution. The experiment determines the enzyme activities of SOD, POD, CAT, GSH and the content of MDA,proline, total phenols and cadmium content. The results showed that the combined pollution of clethodim and cadmium had more damage to celery, compared to the single factor treatment. The enzymes hurted was one of toxic mechanism of clethodim and cadmium, celery showed the increase of total phenols, proline and GSH, which could alleviate the oxidative damage and decrease the content of MDA. There was a certain antagonism between clethodim and cadmium, Clethodim had certain inhibitory effect on the enrichment of cadmium in celery, when the concentration of cadmium was 0.7 mg/kg, the inhibitory effect was obvious.
引文
[1]解庆娓.镉、铅污染对芹菜生长发育的影响[D].南昌:江西农业大学,2014.
[2]GOUNDEN D,KISTEN K,MOODLEY R,et al.Impact of spiked concentrations of Cd,Pb,As and Zn in growth medium on elemental uptake of Nasturtium officinale(Watercress)[J].Journal of Environmental Science and Health,Part B:Pesticides,Food Contaminants,and Agricultural Wastes,2016,51(1):1-7.
[3]陈德敏,薛婧媛.中国土壤污染现状与法律责任解读[J].重庆大学学报(社会科学版),2008,14(1):93-97.
[4]陈元琦.重金属镉与除草剂丁草胺在蔬菜中的吸收富集与毒性效应研究[D].郑州:河南农业大学,2009.
[5]徐尚成.环己二酮类除草剂及其合成化学[J].农药,1990,29(4):31-34.
[6]杨玉廷,高爽,张宗俭,等.除草剂烯草酮的应用技术研究[J].农药,2005,44(4):186-189.
[7]MCLAUGHLIN M J,PARKER D R,CLARKE J M.Metals and micronutrients—food safety issues[J].Field Crops Research,1998,60(1-2):143-163.
[8]何俊瑜,任艳芳.镉胁迫对水稻种子萌发、幼苗生长和淀粉酶活性的影响[J].华北农学报,2008,23(增刊):131-134.
[9]于方明,仇荣亮,汤叶涛,等.Cd对小白菜生长及氮素代谢的影响研究[J].环境科学,2008,29(2):506-511.
[10]黄欣,陆贻通.镉对土壤丁草胺降解作用的影响[J].环境污染与防治,1991,13(1):13-14,5.
[11]陈莹莹,王金花,陆贻通.丁草胺与镉复合污染对土壤呼吸强度的影响[J].环境污染与防治,2006,28(10):723-730.
[12]潘攀,杨俊诚,邓世槐,等.重金属与农药复合污染研究现状及展望[J].农业环境科学学报,2011,30(10):1925-1929.
[13]郭林华,王鹏.除草剂烯草酮的合成研究进展[J].现代农药,2006,5(1):5-8.
[14]周其文,师荣光,韩允磊,等.天津市郊不同利用方式农田土壤镉的累积特征及生态风险评估[J].中国农学通报,2014,30(36):182-187.
[15]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003.
[17]黄爱缨,吴珍龄.水稻谷胱甘肽过氧化物酶的测定法[J].西南农业大学学报,1999,21(4):24-27.
[18]李英涛.上海青对四种不同类型农药胁迫的抗氧化防御反应研究[D].郑州:河南农业大学,2013.
[19]GREENBERG J T,MONACH P,CHOU J H,et al.Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli[J].Proceedings of the National Academy of Sciences of the United States of America,1990,87(16):6181-6185.
[20]邱龙,马崇烈,刘博林,等.耐除草剂转基因作物研究现状及发展前景[J].中国农业科学,2012,45(12):2357-2363.
[21]GUNES A,INAL A,ALPASLAN M,et al.Salicylic acid induced changes on some physiological parameters symptomatic oxidative stress and mineral nutrition in maze(Zea mays L)grown under salinity[J].Plant Physil,2007,164(6):728-736.
[22]ROMERO-PUERTAS M C,RODRIGUEZ-SERRANO M,CORPAS F J,et al.Cadmium-induced subcellular accumulation of O2-and H2O2in pea leaves[J].Plant Cell and Environment,2004,27(9):1122-1134.
[23]何学利.植物体内的保护酶系统[J].现代农业科技,2010(10):37-38.
[24]杨居荣,贺建群,蒋婉茹.Cd污染对植物生理生化的影响[J].农业环境保护,1995,14(5):193-197.
[25]郭华武.铅、汞与除草剂丁草胺复合污染对油麦菜的毒性效应研究[D].郑州:河南农业大学,2010.
[26]DIAS M C,MONTEIRO C,MOUTINHO-PEREIRA J,et al.Cadmium toxicity affects photosynthesis and plant growth at different levels[J].Acta Physiologiae Plantarum,2013,35:1281-1289.
[27]GARRIDO F,ILLERA V,CAMPBELL C G,et al.Regulating the mobility of Cd,Cu and Pb in an acid soil with amendments of phosphogypsum,sugar foam,and phosphoric rock[J].European Journal of Soil Science,2006,57(2):95-105.
[28]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[29]段喜华,唐中华,郭晓瑞.植物谷胱甘肽的生物合成及其生物学功能[J].植物研究,2009,30(1):98-105.
[30]臧丽丽.草除灵对甘蓝型油菜苗期生长的影响及生理调控研究[D].杭州:浙江大学,2015.
[31]张楠楠,汤勇铮,马菲,等.电化学研究铝及其纳米Al(13)对谷胱甘肽还原酶活性的影响[J].分析化学,2012,40(4):584-588.
[32]金春英,崔京兰,崔胜云.氧化型谷胱甘肽对还原型谷胱甘肽清除自由基的协同作用[J].分析化学,2009,37(9):1349-1353.
[33]姜莉莉,史晓斌.ACCase抑制剂类除草剂的作用机理[J].农药研究与应用,2010,14(4):14-17.
[34]周东美,王慎强,陈怀满.土壤中有机污染物-重金属复合污染的交互作用[J].土壤与环境,2000,9(2):143-145.
[35]周垂帆.重金属和草甘膦复合污染生态毒理研究[D].南京:南京林业大学,2013.
[2]GOUNDEN D,KISTEN K,MOODLEY R,et al.Impact of spiked concentrations of Cd,Pb,As and Zn in growth medium on elemental uptake of Nasturtium officinale(Watercress)[J].Journal of Environmental Science and Health,Part B:Pesticides,Food Contaminants,and Agricultural Wastes,2016,51(1):1-7.
[3]陈德敏,薛婧媛.中国土壤污染现状与法律责任解读[J].重庆大学学报(社会科学版),2008,14(1):93-97.
[4]陈元琦.重金属镉与除草剂丁草胺在蔬菜中的吸收富集与毒性效应研究[D].郑州:河南农业大学,2009.
[5]徐尚成.环己二酮类除草剂及其合成化学[J].农药,1990,29(4):31-34.
[6]杨玉廷,高爽,张宗俭,等.除草剂烯草酮的应用技术研究[J].农药,2005,44(4):186-189.
[7]MCLAUGHLIN M J,PARKER D R,CLARKE J M.Metals and micronutrients—food safety issues[J].Field Crops Research,1998,60(1-2):143-163.
[8]何俊瑜,任艳芳.镉胁迫对水稻种子萌发、幼苗生长和淀粉酶活性的影响[J].华北农学报,2008,23(增刊):131-134.
[9]于方明,仇荣亮,汤叶涛,等.Cd对小白菜生长及氮素代谢的影响研究[J].环境科学,2008,29(2):506-511.
[10]黄欣,陆贻通.镉对土壤丁草胺降解作用的影响[J].环境污染与防治,1991,13(1):13-14,5.
[11]陈莹莹,王金花,陆贻通.丁草胺与镉复合污染对土壤呼吸强度的影响[J].环境污染与防治,2006,28(10):723-730.
[12]潘攀,杨俊诚,邓世槐,等.重金属与农药复合污染研究现状及展望[J].农业环境科学学报,2011,30(10):1925-1929.
[13]郭林华,王鹏.除草剂烯草酮的合成研究进展[J].现代农药,2006,5(1):5-8.
[14]周其文,师荣光,韩允磊,等.天津市郊不同利用方式农田土壤镉的累积特征及生态风险评估[J].中国农学通报,2014,30(36):182-187.
[15]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003.
[17]黄爱缨,吴珍龄.水稻谷胱甘肽过氧化物酶的测定法[J].西南农业大学学报,1999,21(4):24-27.
[18]李英涛.上海青对四种不同类型农药胁迫的抗氧化防御反应研究[D].郑州:河南农业大学,2013.
[19]GREENBERG J T,MONACH P,CHOU J H,et al.Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli[J].Proceedings of the National Academy of Sciences of the United States of America,1990,87(16):6181-6185.
[20]邱龙,马崇烈,刘博林,等.耐除草剂转基因作物研究现状及发展前景[J].中国农业科学,2012,45(12):2357-2363.
[21]GUNES A,INAL A,ALPASLAN M,et al.Salicylic acid induced changes on some physiological parameters symptomatic oxidative stress and mineral nutrition in maze(Zea mays L)grown under salinity[J].Plant Physil,2007,164(6):728-736.
[22]ROMERO-PUERTAS M C,RODRIGUEZ-SERRANO M,CORPAS F J,et al.Cadmium-induced subcellular accumulation of O2-and H2O2in pea leaves[J].Plant Cell and Environment,2004,27(9):1122-1134.
[23]何学利.植物体内的保护酶系统[J].现代农业科技,2010(10):37-38.
[24]杨居荣,贺建群,蒋婉茹.Cd污染对植物生理生化的影响[J].农业环境保护,1995,14(5):193-197.
[25]郭华武.铅、汞与除草剂丁草胺复合污染对油麦菜的毒性效应研究[D].郑州:河南农业大学,2010.
[26]DIAS M C,MONTEIRO C,MOUTINHO-PEREIRA J,et al.Cadmium toxicity affects photosynthesis and plant growth at different levels[J].Acta Physiologiae Plantarum,2013,35:1281-1289.
[27]GARRIDO F,ILLERA V,CAMPBELL C G,et al.Regulating the mobility of Cd,Cu and Pb in an acid soil with amendments of phosphogypsum,sugar foam,and phosphoric rock[J].European Journal of Soil Science,2006,57(2):95-105.
[28]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[29]段喜华,唐中华,郭晓瑞.植物谷胱甘肽的生物合成及其生物学功能[J].植物研究,2009,30(1):98-105.
[30]臧丽丽.草除灵对甘蓝型油菜苗期生长的影响及生理调控研究[D].杭州:浙江大学,2015.
[31]张楠楠,汤勇铮,马菲,等.电化学研究铝及其纳米Al(13)对谷胱甘肽还原酶活性的影响[J].分析化学,2012,40(4):584-588.
[32]金春英,崔京兰,崔胜云.氧化型谷胱甘肽对还原型谷胱甘肽清除自由基的协同作用[J].分析化学,2009,37(9):1349-1353.
[33]姜莉莉,史晓斌.ACCase抑制剂类除草剂的作用机理[J].农药研究与应用,2010,14(4):14-17.
[34]周东美,王慎强,陈怀满.土壤中有机污染物-重金属复合污染的交互作用[J].土壤与环境,2000,9(2):143-145.
[35]周垂帆.重金属和草甘膦复合污染生态毒理研究[D].南京:南京林业大学,2013.