豆薯多糖对大鳞副泥鳅非特异性免疫的影响
摘要
实验组大鳞副泥鳅分别体腔注射5、10、20 g/L和40 g/L豆薯多糖溶液(0.2 m L/尾),对照组注射等体积的0.8%Na Cl溶液,在实验开始后第7、14、21 d和28 d,取样测定5个处理组的红细胞总数(RBC)和白细胞总数(WBC),血清酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LSZ)活性和补体C3含量;并在实验开始后第7 d,从每个处理组中随机采取大鳞副泥鳅15尾,每尾体腔注射1×108 CFU/m L的嗜水气单胞菌悬液0.1 m L,记录接种7 d后各组鱼的累积死亡率,然后计算免疫保护率。结果显示:与对照组相比,注射适量的豆薯多糖能显著增加大鳞副泥鳅的RBC和WBC(P<0.05),显著增强血清ACP、AKP、LSZ活性和补体C3含量(P<0.05);明显增强大鳞副泥鳅对嗜水气单胞菌感染的抵抗力。综合考虑各因素,以体腔注射10 g/L剂量较为适宜。
引文
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[2]Pressey,R.Cell wall compositions and enzymes of potatoes,jicamas and Chinese water chestnuts[J].Journal of Food Biochemistry,1993,17:85-95.
[3]葛星星,李义,凌去非.响应面法优化豆薯多糖的超声辅助提取工艺研究[J].中国饲料,2017(21):11-15.
[4]Hultmark D,Seiner H.Insect immunity:Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophera cecropia[J].European Journal of Biochemistry,1980,106:7-16.
[5]傅丽容,贺斌,王雪,等.硝酸铵影响红耳龟和中华条颈龟孵化及血液生理指标的研究[J].四川动物,2012,31(2):252-255.
[6]刘洪丽,陈忠伟,胡庭俊,等.马尾藻多糖对奥尼罗非鱼生长性能、血液生化指标和免疫调节的影响[J].安徽农业科学,2009,37(29):14215-14217.
[7]康洁.马齿苋多糖对草鱼的免疫调节作用[J].贵州农业科学,2012,40(7):162-164.
[8]陈亚军.黄芪多糖对大鳞副泥鳅(Paramisgurnus dabryanus)免疫调节作用的研究[D].苏州:苏州大学,2016.
[9]Lazado C C,Lund L,Pedersen P B,et al.Humoral and mucosal defense molecules rhythmically oscillate during a light-dark cycle in permit,Trachinotus falcatus[J].Fish&Shellfish Immunology,2015,47:902-912.
[10]宋文华,于翔,富丽静,等.黄芪多糖和香菇多糖对草鱼非特异性免疫功能的影响[J].安徽农业科学,2011,39(24):14893-14895,14930.
[11]Yang X,Guo J L,Ye J Y,et al.The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp,Ctenopharyngodon idella[J].Fish&Shellfish Immunology,2015,42:132-137.
[12]Wang E,Xia C,Wang K,et al.Plant polysaccharides used as immunostimulants enhance innate immune response and disease resistance against Aeromonas hydrophila infection in fish[J].Fish&Shellfish Immunology,2016,59:196-202.
[13]王煜恒,徐孝宙,王会聪,等.黄芪多糖对杂交鳢生长性能、免疫能力、抗氧化能力和抗病力的影响[J].动物营养学报,2018,30(4):1447-1456.