黄枝润楠组培快繁技术
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摘要
为了解决外植体易污染、易褐化、诱导分化难等问题,对黄枝润楠组培技术进行了探讨。试验结果表明:用75%酒精处理黄枝润楠的茎段15 s后,再用0.1%的升汞消毒10 min,能大大降低外植体污染率,外植体的存活率达60.0%。黄枝润楠的最佳诱导培养基为MS(改良)+6-BA 0.5 m/L+NAA 0.1 mg/L+蔗糖30 g/L+抗坏血酸1.0 g/L。
In order to avoid the explant browning,easily contaminated and difficultly differentiating and so on,we studied on the tissue culture technique of Machilus versicolora. The experiment results showed that after the stems being treated for 15 s with 75% alcohol,and then by 0. 1% Hg Cl2 disinfected 10 min,which greatly reduced the rate of explants pollution and the explant survival rate was up to 60. 0%. The optimum inducing culture medium of M. versicolora was MS( modified) + 6-BA0. 5 m / L + NAA 0. 1 mg / L + sucrose 30 g / L + ascorbic acid 1. 0 g / L.
In order to avoid the explant browning,easily contaminated and difficultly differentiating and so on,we studied on the tissue culture technique of Machilus versicolora. The experiment results showed that after the stems being treated for 15 s with 75% alcohol,and then by 0. 1% Hg Cl2 disinfected 10 min,which greatly reduced the rate of explants pollution and the explant survival rate was up to 60. 0%. The optimum inducing culture medium of M. versicolora was MS( modified) + 6-BA0. 5 m / L + NAA 0. 1 mg / L + sucrose 30 g / L + ascorbic acid 1. 0 g / L.
引文
[1]邹国名,刘德朝,韩金发,等.黄枝润楠种子育苗技术试验研究[J].林业资源管理,2012(3):119-122.
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[3]黄梓良,杨万平,陈学富.福建茫荡山黄枝润楠群落多样性比较研究[J].亚热带植物科学,2014,43(2):139-143.
[4]鲁萌,阮氏钏,王纪,等.青钱柳茎段腋芽的离体培养技术[J].南京林业大学学报,2013,37(6):6-10.
[5]刘燕,孙超等.鸡爪黄连组培繁殖技术研究初探[J].种子生产,2012,31(8):117-119.
[6]朱丽华,张艺,吴小芹,等.湿地松的组织培养及植株再生[J].南京林业大学学报,2004,28(6):47-50.
[2]陈学富.福建茫荡山黄枝润楠群落植物区系特点研究[J].亚热带植物科学,2009,38(1):46-49.
[3]黄梓良,杨万平,陈学富.福建茫荡山黄枝润楠群落多样性比较研究[J].亚热带植物科学,2014,43(2):139-143.
[4]鲁萌,阮氏钏,王纪,等.青钱柳茎段腋芽的离体培养技术[J].南京林业大学学报,2013,37(6):6-10.
[5]刘燕,孙超等.鸡爪黄连组培繁殖技术研究初探[J].种子生产,2012,31(8):117-119.
[6]朱丽华,张艺,吴小芹,等.湿地松的组织培养及植株再生[J].南京林业大学学报,2004,28(6):47-50.