灵芝咖啡对小鼠免疫功能影响的研究
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摘要
探讨灵芝咖啡对小鼠免疫功能的影响。分别以1.75、3.50、10.5 g/kg bw剂量和蒸馏水经口给予小鼠连续30 d后,测定小鼠胸腺指数、脾指数,进行脾淋巴细胞转化实验、迟发型变态反应实验、抗体生成细胞检测、小鼠碳廓清能力检测、腹腔巨噬细胞吞噬鸡红细胞能力实验及自然杀伤细胞(Natural killer cell,NK)活性检测。结果表明,10.5 g/kg bw剂量组能明显增强刀豆蛋白A(Concanavalin A,Con A)诱导的小鼠脾淋巴细胞增值能力、迟发型变态反应、抗体生成能力、碳廓清能力及NK细胞活性,并能升高小鼠血清溶血素水平。结论证明灵芝咖啡具有增强免疫力作用。
The effects of Ganoderma lucidum coffee on immunologic function in mice were studied in this paper. ICR mice were lavaged continuously at the doses of 1.75, 3.50, 10.5 g/kg bw and distilled water for 30 days, and then the following experiments were conducted: lymphocyte transformation capacity induced by concanavalin(Con A), spleen/body and thymus/body weight ratio, delayed type hypersensitivity(DTH), antibody forming spleen cells(AFPC), activities of antibodies(hemolysin) against sheep red blood ceils, carbon clearance ability, mononuclear phagocyte functionality and NK cell activity. The results showed that compared with control group, Ganoderma lucidum coffee, at the dose of 10.5 g/kg bw, could significantly enhance the function of lymphocyte transformation capacity induced by Con A, DTH, the productive ability of antibody forming cells, carbon learance ability, NK cell activity and the serum hemolysin level. It proved that Ganoderma lucidum coffee could enhance the immunologic function in mice.
The effects of Ganoderma lucidum coffee on immunologic function in mice were studied in this paper. ICR mice were lavaged continuously at the doses of 1.75, 3.50, 10.5 g/kg bw and distilled water for 30 days, and then the following experiments were conducted: lymphocyte transformation capacity induced by concanavalin(Con A), spleen/body and thymus/body weight ratio, delayed type hypersensitivity(DTH), antibody forming spleen cells(AFPC), activities of antibodies(hemolysin) against sheep red blood ceils, carbon clearance ability, mononuclear phagocyte functionality and NK cell activity. The results showed that compared with control group, Ganoderma lucidum coffee, at the dose of 10.5 g/kg bw, could significantly enhance the function of lymphocyte transformation capacity induced by Con A, DTH, the productive ability of antibody forming cells, carbon learance ability, NK cell activity and the serum hemolysin level. It proved that Ganoderma lucidum coffee could enhance the immunologic function in mice.
引文
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[2]林志彬.灵芝的现代研究(第4版)[M].北京:北京大学医学出版社,2015:第1版前言
[3]张蕾,王瑛,朱惠照,等.赤灵芝新菌株“仙芝2号”的选育[J].食用菌学报,2014,21(1):15-20
[4]YUE GG,FUNG KP,LEUNG PC,et al.Comparative studies on the immunomodulatory and antitumor activities of the different parts of fruiting body of Ganoderma lucidum and Ganoderma spores[J].Phytother Res,2008,22(10):1282-1291
[5]张荣标,陈润,陈冠敏.灵芝多糖对小鼠免疫功能影响的实验研究[J].预防医学论坛,2012,18(12):916-920
[6]WANG JL,GU TY,ZHONG JJ,et al.Enhanced recovery of antitumor ganoderic acid T from Ganoderma lucidum mycelia by novel chemical conversion strategy[J].Biotechnol Bioeng,2012,109(3):754-762
[7]林志彬.灵芝抗肿瘤作用的免疫学机制及其临床应用[J].中国药理学与毒理学杂志,2015,(6):865-882.
[8]LI X,LIANG J.Ganoderma lucidum polysaccharide prevents oxidation and skin aging[J].Chinese Journal of Tissue Engineering Research,2013,41(17):7272-7277
[9]JIA J,ZHANG X,HU YS,et al.Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats[J].Food Chem,2009,115(1):32-36
[10]LI WJ,NIE SP,PENG XP,et al.Ganoderma atrum polysaccharide improves age-related oxidative stress and immune impairment in mice[J].J Agric Food Chem,2012,60(6):1413-1418
[11]包焜,陈萍,毕云枫,等.灵芝活性组分在小鼠体内的降糖功效研究[J].中国兽药杂志,2015,49(1):29-32
[12]朱科学,聂少平,宋丹,等.黑灵芝多糖对Ⅱ型糖尿病大鼠血糖、血脂及肠系膜上动脉病变改善作用[J].食品科学,2013,34(23):300-304
[13]李国鹏,何红艳,罗心平,等.咖啡营养特性及营养诊断研究进展[J].中国农学通报,2009,25(1):248-250
[14]杨巍.咖啡碱的药理作用与开发利用前景[J].茶业科学技术,2006,(4):9-11
[15]李海霞,陈榕,周丹,等.咖啡因的合成及其药理作用的研究进展[J].华西药学杂志,2011,26(2):182-187
[16]林志彬.灵芝的现代研究(第四版)[M].北京:北京大学医学出版社,2015:251-278
[17]张旻璐,包海鹰,于晓风,等.灵芝超微粉对小鼠免疫功能的影响[J].人参研究,2014,(2):20-22
[18]李秋涵,洪志丹.富硒灵芝超微粉抗肿瘤及免疫调节功能研究[J].实用药物与临床,2012,15(2):75-77
[19]陈士瑜,陈海英.蕈菌医方集成[M].上海:上海科学技术文献出版社,2000:368