凤丹皮总苷高效液相色谱特征指纹图谱研究
|
摘要
目的建立凤丹皮总苷的指纹图谱,为科学评价及有效控制其质量提供可靠依据。方法采用Agilent TC-C_(18)(2)(250mm×4.6mm,5μm)色谱柱;乙腈(A)-0.05%磷酸水(B)为流动相,梯度洗脱,流速1.0mL/min,检测波长230nm,进样容积20μL。结果建立了凤丹皮总苷高效液相色谱特征指纹图谱的共有模式,指定18个共有峰,统计结果显示,11批凤丹皮总苷样品高效液相色谱特征指纹图谱相似度均大于0.80。结论建立的特征指纹图谱方法快速、准确、简便,可用于凤丹皮总苷的质量控制。
Objective To investigate the fingerprint of total glycosides in Paeonia ostii peel,and to provide a reliable reference for its scientific evaluation and effective quality control.Methods High-performance liquid chromatography(HPLC)was performed on an Agilent TC-C_(18)(2)column(250mm×4.6mm,5μm)with a mobile phase of acetonitrile(A)-0.05% phosphoric acid(B)for gradient elution at a flow rate of 1.0mL/min,a detection wavelength of 230 nm,and a sample volume of 20μL.Results The common pattern of the characteristic fingerprint of total glycosides in Paeonia ostii peel based on HPLC was established,and a total of 18 common peaks were identified.The statistical results showed that the degree of similarity in the characteristic fingerprint of 11 batches of Paeonia ostii peel based on HPCL was higher than 0.80.Conclusion The method for establishing characteristic fingerprint is quick,accurate,and simple and can be used for the quality control of total glycosides in Paeonia ostii peel.
Objective To investigate the fingerprint of total glycosides in Paeonia ostii peel,and to provide a reliable reference for its scientific evaluation and effective quality control.Methods High-performance liquid chromatography(HPLC)was performed on an Agilent TC-C_(18)(2)column(250mm×4.6mm,5μm)with a mobile phase of acetonitrile(A)-0.05% phosphoric acid(B)for gradient elution at a flow rate of 1.0mL/min,a detection wavelength of 230 nm,and a sample volume of 20μL.Results The common pattern of the characteristic fingerprint of total glycosides in Paeonia ostii peel based on HPLC was established,and a total of 18 common peaks were identified.The statistical results showed that the degree of similarity in the characteristic fingerprint of 11 batches of Paeonia ostii peel based on HPCL was higher than 0.80.Conclusion The method for establishing characteristic fingerprint is quick,accurate,and simple and can be used for the quality control of total glycosides in Paeonia ostii peel.
引文
[1]杨利民,张永刚,林红梅,等.中药材质量形成理论与控制技术研究进展[J].吉林农业大学学报,2012,34(2):119-124,129.
[2]王桐生,王艳华,吴德玲,等.丹皮不同提取物对子宫内膜异位症模型大鼠血液流变学及细胞因子的影响[J].中药材,2012,35(10):1649-1652.
[3]张伟,王艳华,吴德玲,等.牡丹皮抗子宫内膜异位症活性部位化学成分研究[J].中成药,2014,36(1):119-121.
[4]孙萌,周长征.丹皮总苷对小鼠急性心肌缺血保护作用[J].辽宁中医药大学学报,2014,16(1):41-43.
[5]王艳华,吴德玲,刘劲松,等.牡丹皮中丹皮总苷的提取工艺研究[J].安徽中医学院学报,2012,31(2):69-71.
[6]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671-675.
[7]高新彪,孙磊,乔善义,等.牡丹皮HPLC指纹图谱研究[J].中草药,2013,44(7):900-904.
[8]许舜军,李鹏,杨柳,等.牡丹皮高效液相色谱指纹图谱研究[J].中国中药杂志,2006,31(20):1677-1680.
[9]李家春,孙兰,李红娟,等.桂枝茯苓胶囊HPLC指纹图谱研究[J].中草药,2013,43(7):1333-1335.
[10]孙永成,窦霞,尚强,等.桂枝茯苓胶囊(精制)多成分指纹图谱的研究[J].世界科学技术:中医药现代化,2010,12(2):294-297.
[11]王婷婷,陈晓辉,胡庆庆,等.白芷质量的HPLC指纹图谱评价方法[J].药学学报,2006,41(8):747-751.
[12]黄样增,曾常青,朱爽,等.钩藤生物碱HPLC指纹图谱构建及不同基原种钩藤药材的鉴别[J].中药材,2014,37(2):233-236.
[2]王桐生,王艳华,吴德玲,等.丹皮不同提取物对子宫内膜异位症模型大鼠血液流变学及细胞因子的影响[J].中药材,2012,35(10):1649-1652.
[3]张伟,王艳华,吴德玲,等.牡丹皮抗子宫内膜异位症活性部位化学成分研究[J].中成药,2014,36(1):119-121.
[4]孙萌,周长征.丹皮总苷对小鼠急性心肌缺血保护作用[J].辽宁中医药大学学报,2014,16(1):41-43.
[5]王艳华,吴德玲,刘劲松,等.牡丹皮中丹皮总苷的提取工艺研究[J].安徽中医学院学报,2012,31(2):69-71.
[6]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671-675.
[7]高新彪,孙磊,乔善义,等.牡丹皮HPLC指纹图谱研究[J].中草药,2013,44(7):900-904.
[8]许舜军,李鹏,杨柳,等.牡丹皮高效液相色谱指纹图谱研究[J].中国中药杂志,2006,31(20):1677-1680.
[9]李家春,孙兰,李红娟,等.桂枝茯苓胶囊HPLC指纹图谱研究[J].中草药,2013,43(7):1333-1335.
[10]孙永成,窦霞,尚强,等.桂枝茯苓胶囊(精制)多成分指纹图谱的研究[J].世界科学技术:中医药现代化,2010,12(2):294-297.
[11]王婷婷,陈晓辉,胡庆庆,等.白芷质量的HPLC指纹图谱评价方法[J].药学学报,2006,41(8):747-751.
[12]黄样增,曾常青,朱爽,等.钩藤生物碱HPLC指纹图谱构建及不同基原种钩藤药材的鉴别[J].中药材,2014,37(2):233-236.