薏苡仁油对小鼠免疫功能影响的研究
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摘要
探讨薏苡仁油对小鼠免疫功能的影响。分别以低、中、高剂量薏苡仁油(0.17、0.33、1.00 g/kg)和食用植物油连续经口灌胃ICR小鼠30 d,测定胸腺指数与脾指数,并进行脾淋巴细胞转化实验、迟发性变态反应实验、血清溶血素实验和抗体生成细胞检测、碳廓清实验、腹腔巨噬细胞吞噬鸡红细胞实验和NK细胞活性实验。结果表明:高剂量(1.00 g/kg)组能显著增强脾淋巴细胞增殖能力(以刀豆蛋白A(ConA)诱导)和迟发性变态反应(以二硝基氟苯(DNFB)诱导),升高小鼠血清溶血素水平,增强小鼠抗体生成能力和NK细胞活性。证明薏苡仁油具有增强免疫力作用。
The effects of coix oil on immunologic function in mice were studied.At the doses of coix oil0.17,0.33,1.00 g/kg( oral gavage) and edible vegetable oil( oral gavage) for 30 d and then the following experiments were conducted: spleen/body weight and thymus/body weight ratio,lymphocyte transformation capacity induced by concanavalin( ConA),delayed type hypersensitivity( DTH),antibody forming spleen cells( AFPC),activities of antibodies( hemolysin) against sheep red blood cells,carbon clearance ability,mononuclear phagocyte functionality and NK cell activity.The results showed that compared with control group,coix oil at the dose of 1.00 g/kg could enhance the capacity of lymphocyte transformation induced by ConA,DTH( induction with DNFB),NK cell activity,AFPC and the serum hemolysin level.It proved that coix oil could enhance the immunologic function in mice.
The effects of coix oil on immunologic function in mice were studied.At the doses of coix oil0.17,0.33,1.00 g/kg( oral gavage) and edible vegetable oil( oral gavage) for 30 d and then the following experiments were conducted: spleen/body weight and thymus/body weight ratio,lymphocyte transformation capacity induced by concanavalin( ConA),delayed type hypersensitivity( DTH),antibody forming spleen cells( AFPC),activities of antibodies( hemolysin) against sheep red blood cells,carbon clearance ability,mononuclear phagocyte functionality and NK cell activity.The results showed that compared with control group,coix oil at the dose of 1.00 g/kg could enhance the capacity of lymphocyte transformation induced by ConA,DTH( induction with DNFB),NK cell activity,AFPC and the serum hemolysin level.It proved that coix oil could enhance the immunologic function in mice.
引文
[1]UKITA T,TANIMURA A. Stuides on the antitumor componenit in the seeds of Coix lachryma-jobi L. var. mayuen(Roman.)Stapf. isolation and antitumor activity of coixenolide[J]. Chem Pharm Bull,1961,9(1):43-46.
[2]TOKUDA H,MATSUMOTO T,KONISHIMA T,et al. Inhibitory effects on Epstein-Barr virus activation and antitumor promoting activities of coix seed[J]. Planta Medica,1990,56(6):653-654.
[3]张栋霞,张涛. GC/MSD分析薏苡仁油组份[J].粮食与油脂,2001(1):42-43.
[4]陈碧莲,祝明,陈勇,等. GC-MS分析薏苡仁油中不皂化物的主要成分[J].中成药,2009,31(6):953-954.
[5]谢春英,林乐维.超临界CO2流体萃取薏苡仁油的GC-MS分析[J].中药材,2011,34(8):1234-1236.
[6]吴岩,潘沛,王彧杰,等.康莱特注射液对Lewis肺癌小鼠免疫功能的影响[J].上海交通大学学报(医学版),2009,29(12):1455-1458.
[7]景俊杰,李小玲,南月清,等.康莱特注射液对肿瘤治疗的作用研究进展[J].中国药物与临床,2013,13(11):1447-1448.
[8]张荣标,陈润,陈冠敏.灵芝多糖对小鼠免疫功能影响的实验研究[J].预防医学论坛,2012,12(5):916-920.
[9]张迅捷,陈冠敏,陈润.紫菜多糖对小鼠免疫功能影响的研究[J].现代预防医学,2017,14(3):2601-2603.
[10]陆蕴,张仲苗,章荣华.薏苡仁油抗肿瘤作用研究[J].中药药理与临床,1999,15(6):21-23.
[11]姚玉龙,陈秀华,任文龙,等.康莱特软胶囊对小鼠的免疫促进作用研究[J].中药新药和临床药理,2002,13(4):233-235.
[12]肖小年,易海斌,郭烨,等.薏苡仁油微乳对雄性小鼠脏器的影响[J].南昌大学学报(理科版),2012,36(1):65-68.
[13]范伟忠,章荣华,傅剑云.薏苡仁油的毒性研究及安全性评价[J].上海预防医学杂志,2000,12(4):178-179.
[2]TOKUDA H,MATSUMOTO T,KONISHIMA T,et al. Inhibitory effects on Epstein-Barr virus activation and antitumor promoting activities of coix seed[J]. Planta Medica,1990,56(6):653-654.
[3]张栋霞,张涛. GC/MSD分析薏苡仁油组份[J].粮食与油脂,2001(1):42-43.
[4]陈碧莲,祝明,陈勇,等. GC-MS分析薏苡仁油中不皂化物的主要成分[J].中成药,2009,31(6):953-954.
[5]谢春英,林乐维.超临界CO2流体萃取薏苡仁油的GC-MS分析[J].中药材,2011,34(8):1234-1236.
[6]吴岩,潘沛,王彧杰,等.康莱特注射液对Lewis肺癌小鼠免疫功能的影响[J].上海交通大学学报(医学版),2009,29(12):1455-1458.
[7]景俊杰,李小玲,南月清,等.康莱特注射液对肿瘤治疗的作用研究进展[J].中国药物与临床,2013,13(11):1447-1448.
[8]张荣标,陈润,陈冠敏.灵芝多糖对小鼠免疫功能影响的实验研究[J].预防医学论坛,2012,12(5):916-920.
[9]张迅捷,陈冠敏,陈润.紫菜多糖对小鼠免疫功能影响的研究[J].现代预防医学,2017,14(3):2601-2603.
[10]陆蕴,张仲苗,章荣华.薏苡仁油抗肿瘤作用研究[J].中药药理与临床,1999,15(6):21-23.
[11]姚玉龙,陈秀华,任文龙,等.康莱特软胶囊对小鼠的免疫促进作用研究[J].中药新药和临床药理,2002,13(4):233-235.
[12]肖小年,易海斌,郭烨,等.薏苡仁油微乳对雄性小鼠脏器的影响[J].南昌大学学报(理科版),2012,36(1):65-68.
[13]范伟忠,章荣华,傅剑云.薏苡仁油的毒性研究及安全性评价[J].上海预防医学杂志,2000,12(4):178-179.