苯酚-硫酸法与蒽酮-硫酸法测定麦冬中麦冬多糖含量的比较研究
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摘要
比较苯酚-硫酸法和蒽酮-硫酸法测定麦冬多糖含量的差异,并对两种方法的测定条件进行优化。结果表明:苯酚-硫酸法最佳测定条件为苯酚质量分数6%、硫酸用量5 mL、反应温度50℃、反应时间20 min,测得麦冬中多糖含量为42.96%。蒽酮-硫酸法最佳测定条件为蒽酮-硫酸用量4 mL、反应温度100℃、反应时间15 min,测得麦冬中多糖含量为38.56%。在10～100μg/mL范围内,苯酚-硫酸法呈良好的线性关系(R~2=0.999 1),明显优于蒽酮-硫酸法线性关系(R~2=0.996 4);苯酚-硫酸法测定麦冬多糖精密度、重复性和加样回收率均优于蒽酮-硫酸法,在0～120min内稳定性较蒽酮-硫酸法差别不大。综合考虑,苯酚-硫酸法与蒽酮-硫酸法均适用于麦冬多糖成分的测定,但苯酚-硫酸法准确性与稳定性更好,故在麦冬中麦冬多糖含量测定时可优先考虑苯酚-硫酸法。
To compare the difference in determination content between phenol-sulfuric and anthrone-sulfuric acid method for polysaccharide in Radix Ophiopogonis. The results show that the optimum conditions for the determination of phenol-sulfuricacid method were 6% aqueous phenol solution, 5 mL of concentrated sulfuric acid, reaction temperature 50 ℃ and reaction time 20 min, the content of polysaccharide was 42.96%. The optimum conditions for the determination of anthrone-sulfuricacid method were 4 mL anthrone-sulfuric, reaction temperature 100 ℃ and reaction time 15 min, the content of polysaccharide was 38.56%. The linearity of the phenol-sulfuric acid method(R~2=0.999 1) better than anthrone-sulfuric acid method(R~2=0.996 4) in the range of 10 ～ 100 μg/mL. The phenol-sulphuric acid method shows better accuracy, reproducibility, stability(0 ～ 120 min) and recovery, which was better than that of the anthrone-sulfuric acid method. In conclusion, both the phenol-sulfuricand anthrone-sulfuric acid methods could determine the polysaccharide content of samples. The phenol-sulfuric acid method was more accurate and stability for the determination of polysaccharide in Radix Ophiopogonis.
To compare the difference in determination content between phenol-sulfuric and anthrone-sulfuric acid method for polysaccharide in Radix Ophiopogonis. The results show that the optimum conditions for the determination of phenol-sulfuricacid method were 6% aqueous phenol solution, 5 mL of concentrated sulfuric acid, reaction temperature 50 ℃ and reaction time 20 min, the content of polysaccharide was 42.96%. The optimum conditions for the determination of anthrone-sulfuricacid method were 4 mL anthrone-sulfuric, reaction temperature 100 ℃ and reaction time 15 min, the content of polysaccharide was 38.56%. The linearity of the phenol-sulfuric acid method(R~2=0.999 1) better than anthrone-sulfuric acid method(R~2=0.996 4) in the range of 10 ～ 100 μg/mL. The phenol-sulphuric acid method shows better accuracy, reproducibility, stability(0 ～ 120 min) and recovery, which was better than that of the anthrone-sulfuric acid method. In conclusion, both the phenol-sulfuricand anthrone-sulfuric acid methods could determine the polysaccharide content of samples. The phenol-sulfuric acid method was more accurate and stability for the determination of polysaccharide in Radix Ophiopogonis.
引文
[1]黄光辉,孙连娜.麦冬多糖的研究进展[J].现代药物与临床,2012,27(5):523-529.
[2]Zhou Y f,Qi J,Zhu D N,et al.Homoisoflavonoids from Ophiopogon japonicus and its oxygen free radicals(OFRs)scavenging effects[J].Chin J Nat Med,2008,6(3):201-204.
[3]Li N,Zhang K W,Zeng KW,et al.Antiinflammatory homoisoflavonoids from the tuberous roots of Ophiopogon japonicas[J].Fitoterapia,2012,83(6):1042-1045.
[4]刘立萍,李然.纯补胃阴论麦冬[J].辽宁中医杂志,2011,38(1):132-133.
[5]张璐欣,周学谦,李德坤,等.麦冬多糖的化学组成、分析方法和药理作用研究进展[J].药物评价究,2017,40(2):279-284.
[6]Liu Y,Liang Y,Zhou Y,et al.Experimental evidence for ion accumulation time affecting qualitative and quantitative analysis of Ophiopogons in Ophiopogon extract by hybrid ion trap time-of-flight mass spectrometry[J].Chromatographia,2013,76(15/16):949-958.
[7]Xie T,Liang Y,Hao H,et al.Rapid identification Ophiopogonones in Ophiopogon japonicus extract with a practical technique of mass defect spectrometry[J].J Chromatogr A,2012,1227(5):234-244.
[8]时潇丽,姚春霞,林晓,等.多糖药物应用与研究进展[J].中国新药杂志,2014,23(9):1057-1062.
[9]白瑞斌,马玉玲,张培,等.苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量[J].中国药房,2017,28(21):2974-2978.
[10]靳淑敏,周娜,董振咏,等.苯酚-硫酸比色法测定三黄糖敏汤中总多糖含量[J].河北医科大学学报,2015,36(8):980-982.
[11]王晶,王春国,李冰,等.苯酚-硫酸法测of Ophiopogonins and filtering based on high resolution mass定慈姑中多糖的含量[J].吉林中医药,2017,37(12):1258-1260.
[12]申希峰,黄杰涛,张莲姬.蒽酮-硫酸法测定榛花多糖含量条件的优化[J].食品研究与开发,2017,38(18):150-154.
[13]张媛媛,张彬.苯酚-硫酸法与蒽酮-硫酸法测定绿茶茶多糖的比较研究[J].食品科学,2016,37(4):158-163.
[14]王文洁,唐炜,俞玲娜,等.蒽酮-硫酸法与苯酚-硫酸法测定凉粉草多糖的比较[J].食品科技,2017,42(9):274-279.
[15]包志华,高建萍,徐天宇.DNS比色法测定菥蓂子中多糖的含量[J].内蒙古农业大学学报(自然科学版),2013,34(5):23-25.
[16]蔡凌云.DNS法测定空心莲子草多糖含量研究[J].凯里学院学报,2017,35(6):58-60.
[17]韩振泰,赵玉娟,刘惠文,等.高效液相色谱法测定灵芝多糖含量[J].中国农业科技导报,2009,11(S1):65-67.
[18]陈亮,彭雅娟,钱一帆,等.高效液相色谱法测定灵芝孢子粉软胶囊中多糖含量的研究[J].时珍国医国药,2007(6):1372-1374.
[19]金凌云,黄样增,吴长辉,等.酶解法检测灵芝咖啡中粗多糖含量的研究[J].食品工业,2015,36(10):206-209.
[20]张卫明,单承莺,姜洪芳,等.酶解法测定山药多糖含量的研究[J].食品科学,2009,30(20):403-405.
[2]Zhou Y f,Qi J,Zhu D N,et al.Homoisoflavonoids from Ophiopogon japonicus and its oxygen free radicals(OFRs)scavenging effects[J].Chin J Nat Med,2008,6(3):201-204.
[3]Li N,Zhang K W,Zeng KW,et al.Antiinflammatory homoisoflavonoids from the tuberous roots of Ophiopogon japonicas[J].Fitoterapia,2012,83(6):1042-1045.
[4]刘立萍,李然.纯补胃阴论麦冬[J].辽宁中医杂志,2011,38(1):132-133.
[5]张璐欣,周学谦,李德坤,等.麦冬多糖的化学组成、分析方法和药理作用研究进展[J].药物评价究,2017,40(2):279-284.
[6]Liu Y,Liang Y,Zhou Y,et al.Experimental evidence for ion accumulation time affecting qualitative and quantitative analysis of Ophiopogons in Ophiopogon extract by hybrid ion trap time-of-flight mass spectrometry[J].Chromatographia,2013,76(15/16):949-958.
[7]Xie T,Liang Y,Hao H,et al.Rapid identification Ophiopogonones in Ophiopogon japonicus extract with a practical technique of mass defect spectrometry[J].J Chromatogr A,2012,1227(5):234-244.
[8]时潇丽,姚春霞,林晓,等.多糖药物应用与研究进展[J].中国新药杂志,2014,23(9):1057-1062.
[9]白瑞斌,马玉玲,张培,等.苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量[J].中国药房,2017,28(21):2974-2978.
[10]靳淑敏,周娜,董振咏,等.苯酚-硫酸比色法测定三黄糖敏汤中总多糖含量[J].河北医科大学学报,2015,36(8):980-982.
[11]王晶,王春国,李冰,等.苯酚-硫酸法测of Ophiopogonins and filtering based on high resolution mass定慈姑中多糖的含量[J].吉林中医药,2017,37(12):1258-1260.
[12]申希峰,黄杰涛,张莲姬.蒽酮-硫酸法测定榛花多糖含量条件的优化[J].食品研究与开发,2017,38(18):150-154.
[13]张媛媛,张彬.苯酚-硫酸法与蒽酮-硫酸法测定绿茶茶多糖的比较研究[J].食品科学,2016,37(4):158-163.
[14]王文洁,唐炜,俞玲娜,等.蒽酮-硫酸法与苯酚-硫酸法测定凉粉草多糖的比较[J].食品科技,2017,42(9):274-279.
[15]包志华,高建萍,徐天宇.DNS比色法测定菥蓂子中多糖的含量[J].内蒙古农业大学学报(自然科学版),2013,34(5):23-25.
[16]蔡凌云.DNS法测定空心莲子草多糖含量研究[J].凯里学院学报,2017,35(6):58-60.
[17]韩振泰,赵玉娟,刘惠文,等.高效液相色谱法测定灵芝多糖含量[J].中国农业科技导报,2009,11(S1):65-67.
[18]陈亮,彭雅娟,钱一帆,等.高效液相色谱法测定灵芝孢子粉软胶囊中多糖含量的研究[J].时珍国医国药,2007(6):1372-1374.
[19]金凌云,黄样增,吴长辉,等.酶解法检测灵芝咖啡中粗多糖含量的研究[J].食品工业,2015,36(10):206-209.
[20]张卫明,单承莺,姜洪芳,等.酶解法测定山药多糖含量的研究[J].食品科学,2009,30(20):403-405.