山楂叶总黄酮对脊髓损伤大鼠运动功能和炎症反应的影响
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摘要
目的:探讨山楂叶总黄酮对脊髓损伤(SCI)大鼠运动功能及炎症反应的影响。方法:选取30只SD大鼠,随机分为假手术组(A组)、SCI组(B组)及山楂叶总黄酮低剂量(5mg/kg)组(C)、中剂量(10mg/kg)组(D组)和高剂量(20mg/kg)组(E组),每组6只。除A组外,其他各组均采用改良Allen’s法复制SCI模型。于SCI后24h、48h、72h,采用Basso-Beattie-Bresnahan(BBB)评估大鼠的运动功能。采用酶联免疫吸附试验(ELISA)法测定脊髓组织中白介素(IL)-1β、IL-10和肿瘤坏死因子(TNF)-α水平。结果:与A组比较,B组BBB评分明显降低,脊髓组织IL-1β、IL-10和TNF-α水平显著升高(P<0.05);随着山楂叶总黄酮浓度的增加,BBB评分逐渐升高,IL-1β、IL-10和TNF-α浓度下降,且与B组比较差异均有统计学意义(均P<0.05)。结论:山楂叶总黄酮能够有效抑制SCI大鼠炎症反应,改善肢体的运动功能。
Objective:To investigate the effect of hawthorn leaves flavonoids on motor function and inflammatory reaction in rats with spinal cord injury(SCI).Methods:Thirty SD rats were randomly divided into sham-operated group,SCI group and hawthorn leaves flavonoids low-dose(5 mg/kg),middle-dose(10 mg/kg) and high-dose(20 mg/kg) groups,with 6 rats in each group.Except for the sham-operated group,the rat SCI model was established by Allen's method.The motor function were evaluated by Basso-BeattieBresnahan(BBB)score.The levels of interleukin(IL)-1β,IL-10 and tumor necrosis factor(TNF)-αwere detected by enzyme linked immunosorbent assay(ELISA).Results:Compared with sham-operated group,the score of BBB in SCI group was significantly decreased,while the levels of IL-1β,IL-10 and TNF-αin spinal cord tissues were increased(P<0.05).The concentrations of IL-1β,IL-10 and TNF-αin spinal cord tissues of the three treatment groups decreased with the increase of the dose of hawthorn leaves flavonoids(P<0.05).Conclusion:Hawthorn leaves flavonoids could inhibit inflammatory response and improve motor function in SCI rats.
Objective:To investigate the effect of hawthorn leaves flavonoids on motor function and inflammatory reaction in rats with spinal cord injury(SCI).Methods:Thirty SD rats were randomly divided into sham-operated group,SCI group and hawthorn leaves flavonoids low-dose(5 mg/kg),middle-dose(10 mg/kg) and high-dose(20 mg/kg) groups,with 6 rats in each group.Except for the sham-operated group,the rat SCI model was established by Allen's method.The motor function were evaluated by Basso-BeattieBresnahan(BBB)score.The levels of interleukin(IL)-1β,IL-10 and tumor necrosis factor(TNF)-αwere detected by enzyme linked immunosorbent assay(ELISA).Results:Compared with sham-operated group,the score of BBB in SCI group was significantly decreased,while the levels of IL-1β,IL-10 and TNF-αin spinal cord tissues were increased(P<0.05).The concentrations of IL-1β,IL-10 and TNF-αin spinal cord tissues of the three treatment groups decreased with the increase of the dose of hawthorn leaves flavonoids(P<0.05).Conclusion:Hawthorn leaves flavonoids could inhibit inflammatory response and improve motor function in SCI rats.
引文
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[15]朱双龙,段会全,刘英富,等.柴胡皂苷a对大鼠急性脊髓损伤的神经保护作用与机制研究[J].中国修复重建外科杂志,2017(7):825-829.
[16]DONNELLY D J,POPOVICH P G.Inflammation and its role in neuroprotection,axonal regeneration and functional recovery after spinal cord injury[J].Experimental Neurology,2008,209(2):378-388.
[17]ZHANG H,WU F,KONG X,et al.Nerve growth factor improves functional recovery by inhibiting endoplasmic reticulum stress-induced neuronal apoptosis in rats with spinal cord injury[J].Journal of Translational Medicine,2014,12(1):130.
[2]HUA Z,LEI C,DU X,et al.Transplantation ofcerebral dopamine neurotrophic factor transducted BMSCs in contusion spinal cord injury of rats:promotion of nerve regeneration by alleviating neuroinflammation[J].Molecular Neurobiology,2016,53(1):187-199.
[3]KIM K T,KIM H J,CHO D C,et al.Substance P stimulates proliferation of spinal neural stem cells in spinal cord injury via the mitogen activated protein kinase signaling pathway[J].Spine Journal,2015,15(9):2055-2065.
[4]马国,蒋学华,黄婷,等.山楂叶总黄酮在大鼠体内的药动学研究[J].中国药学杂志,2007,42(8):613-617.
[5]RENNO W M,BENOV L,KHAN K M.Possible role of antioxidative capacity of(-)-epigallocatechin-3-gallate treatment in morphological and neurobehavioral recovery after sciatic nerve crush injury[J].Journal of Neurosurgery Spine,2017,27(5):1.
[6]TUNCEL M,PALAOGLU S,KAPTANOGLU E,et al.Comparison of the effects of melatonin andmethylprednisolone in experimental spinal cord injury[J].Journal of Neurosurgery,2000,93(1Suppl):77.
[7]陈向荣,游思维,金大地.BBB评分评估脊髓损伤大鼠后肢运动功能的探讨[J].中国脊柱脊髓杂志,2004,14(9):547-549.
[8]MATTUCCI S,SPEIDEL J,LIU J,et al.Basic biomechanics of spinal cord injury-How injuries happen in people and how animal models have informed our understanding[J].Clinical Biomechanics,2018.DIO:10.1016/i.clinbiomech.2018.03.020.
[9]江爱龙,刘荣华,陈兰英,等.山楂药理与临床研究进展[J].武警后勤学院学报(医学版),2009,18(2):154-158.
[10]吴晓光,檀荣方,杨岚,等.山楂叶总黄酮对慢性脑缺血大鼠脑组织c-fos、Bcl-2蛋白表达的影响[J].中国老年学,2016,36(1):33-34.
[11]张晨,吕雷锋,李苗,等.藏红花通过抗凋亡、抗炎和抗水肿机制发挥对大鼠脊髓损伤模型的神经保护作用[J].西安交通大学学报(医学版),2017,38(2):280-289.
[12]CHEN X G,CHEN L H,XU R X.Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1on spinal cord injury rats[J].Official Journal of the International Society for Pediatric Neurosurgery,2018,34(8):1479-1487.
[13]ORR M B,GENSEL J C.Spinalcord injury scarring and Inflammation:Therapies Targeting Glial and Inflammatory Responses[J].Neurotherapeutics,2018(3):1-13.
[14]AMINI P A,AKBARI M,FARAHABADI A,et al.Effect of estrogen therapy on TNF-αand iNOS gene expression in spinal cord injury model[J].Acta Med I-ran,2016,54(5):296-301.
[15]朱双龙,段会全,刘英富,等.柴胡皂苷a对大鼠急性脊髓损伤的神经保护作用与机制研究[J].中国修复重建外科杂志,2017(7):825-829.
[16]DONNELLY D J,POPOVICH P G.Inflammation and its role in neuroprotection,axonal regeneration and functional recovery after spinal cord injury[J].Experimental Neurology,2008,209(2):378-388.
[17]ZHANG H,WU F,KONG X,et al.Nerve growth factor improves functional recovery by inhibiting endoplasmic reticulum stress-induced neuronal apoptosis in rats with spinal cord injury[J].Journal of Translational Medicine,2014,12(1):130.