基于叶绿体DNA accD-psaI序列分析的广西梨地方品种遗传多样性研究
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摘要
为评价广西梨地方品种的遗传多样性水平,对54份广西梨地方品种的叶绿体DNA高变区域accD-psaI进行测序,并与Genbank中42份梨主栽品种(来自白梨、秋子梨、砂梨、新疆梨、西洋梨和选育品种)的accD-psaI序列做比较。对比发现,广西梨地方品种的accD-psaI序列长度为683～936 bp不等,检测出单倍型(H1～H6)数量6个,简约性信息位点1个,单一突变位点5个,插入/缺失(InDels)片段5个,单倍型多样性(Hd)为0.710,核苷酸多样性(Pi)为0.64×10~(-3)。单倍型H1、H2和H3为常见单倍型,其中H1在广西各地分布最为广泛。Median-joining Network图显示,单倍型H4和H5为原始单倍型,H2、H3和H8为较进化单倍型。2个原始单倍型只在横县梨地方品种中检测到,同时横县的单倍型类型最多,推测横县为广西梨起源与多样性中心。
引文
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[4] 岳晓燕,黄新忠,宗宇,等.福建省梨地方品种的遗传多样性研究[J].园艺学报,2015,42(1):119-130
[5] FERRADINI N,LANCIONI H,TORRICELLI R,et al.Characterization and phylogenetic analysis of ancient Italian landraces of pear[J].Frontiers in Plant Science,2017,8:751
[6] LIU J,ZHENG X Y,POTTER D,et al.Genetic diversity and population structure of Pyrus calleryana (Rosaceae)in Zhejiang province,China [J].Biochemical Systematics and Ecology,2012,45:69-78
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[12] WU J,WANG Y T,XU J B,et al.Diversification and independent domestication of Asian and European pears[J].Genome Biology,2018,19(1):77