应用RAPD标记检测淮山种质资源的遗传多样性
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摘要
采用RAPD标记对7份德化淮山种质进行遗传多态性和遗传距离分析,并构建聚类图。从63个RAPD引物中筛出31个多态性较好的组合,扩增得到了156个多态性标记。结果表明,7份淮山种质依据遗传距离可分为2类,组间的遗传距离为0.32,其中,大铭薯、泉淮1513和泉淮1547的亲缘关系较近,组内的平均遗传距离为0.21,另一类组内的平均遗传距离为0.44。对照田间表型可见,聚类结果相近于传统分类结果。利用RAPD标记能有效地分析淮山的遗传多样性,对杂交优势的利用具有较大的指导意义。
To analyze the genetic polymorphism and genetic distance of 7 Dehua Dioscorea opposita germplasm by RAPD markers,and build the cluster diagram,31 combinations with good polymorphism were screened out from 63 RAPD primers,and 156 polymorphic markers were amplified.The results showed that 7 D. opposita germplasms could be divided into two groups according to genetic distance,and the genetic distance between groups was 0.32.Among them,Damingshu,Quanhuai 1513 and Quanhuai 1547 were closely related,with an average genetic distance of 0.21 in the group,the mean genetic distance in the other group was 0.44.Compared with the field phenotype,the clustering results were similar to the traditional classification results.The genetic diversity of D. opposita can be effectively analyzed by RAPD markers,which is of great guiding significance for the utilization of hybridization.
To analyze the genetic polymorphism and genetic distance of 7 Dehua Dioscorea opposita germplasm by RAPD markers,and build the cluster diagram,31 combinations with good polymorphism were screened out from 63 RAPD primers,and 156 polymorphic markers were amplified.The results showed that 7 D. opposita germplasms could be divided into two groups according to genetic distance,and the genetic distance between groups was 0.32.Among them,Damingshu,Quanhuai 1513 and Quanhuai 1547 were closely related,with an average genetic distance of 0.21 in the group,the mean genetic distance in the other group was 0.44.Compared with the field phenotype,the clustering results were similar to the traditional classification results.The genetic diversity of D. opposita can be effectively analyzed by RAPD markers,which is of great guiding significance for the utilization of hybridization.
引文
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[2] 国家药典委员会.中华人民共和国药典(2010年版第一增补本)[S].北京:中国医药科技出版社,2010.
[3] 巩庆平,程广,袁良敏.山药标准化栽培技术[M].北京:中国农业出版社,2004.
[4] 李晶,秦天才.盾叶薯蓣居群亲缘关系的RAPD分析[J].湖北农业科学,2005(4):20-22.
[5] 李明军,徐鑫,张晓丽,等.山药基因组DNA的提取和RAPD反应条件的优化[J].河南师范大学学报(自然科学版),2007,35(1):140-143.
[6] 华树妹,涂前程,雷伏贵.福建山药种质资源遗传多样性的RAPD分析[J].植物遗传资源学报,2009,10(2):195-200.
[7] 黄春洪,杭悦宇,周义锋,等.我国盾叶薯蓣居群遗传结构分析[J].云南植物研究,2003,25(6):641-647.
[8] MIGNOUNA H D,ASIEDU R.A genetic linkage map of Guinea yam (Dioscorea rotundata Poir.) based on isozyme,RAPD and AFLP markers[M].San Diego,California:[s.n.],1999.
[9] SAGHAI-MAROOF M A,SOLIMAN K M,JORGENSEN R A,et al.Ribosomal DNA spacer-length polymorphisms in barley:Mendelian inheritance,chromosomal location,and population dynamics[J].Proceedings of the national academy of sciences of the United States of America,1984,81(24):8014-8018.
[10] NEI M,LI W H.Mathematical model for studying genetic variation in terms of restriction endouncleases[J].Proceedings of the national academy of sciences of the United States of America,1979,76(10):5269-5273.
[11] R Development Core Team.The R project for statistical computing[Z].2015.