腰椎椎间盘退行性变大动物模型的建立方法
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摘要
<正>椎间盘退行性变(IDD)是脊柱外科的常见病和多发病,随着人口老龄化进程的加速,其发生率大幅上升~([1-2])。IDD是引起腰背痛的主要原因~([3-4]),为了寻求治疗腰背痛的有效手段,IDD与椎间盘再生的基础研究近年来受到了广泛关注。由于穿刺或者椎间盘造影会破坏椎间盘完整性,加速退行性变,在人体上进行的椎间盘研究只能进行观察而不能开展直接干预的有创研究,而动物模型在许多方面可以模拟人体IDD的发生过程,从而可
引文
[1]Hoy D,Bain C,Williams G,et al.A systematic review of the global prevalence of low back pain[J].Arthritis Rheum,2012,64(6):2028-2037.
[2]Hoy D,March L,Brooks P,et al.The global burden of low back pain:estimates from the Global Burden of Disease 2010 study[J].Ann Rheum Dis,2014,73(6):968-974.
[3]Molinos M,Almeida CR,Caldeira J,et al.Inflammation in intervertebral disc degeneration and regeneration[J].J R Soc Interface,2015,12(108):20150429.
[4]Oehme D,Goldschlager T,Ghosh P,et al.Cell-based therapies used to treat lumbar degenerative disc disease:a systematic review of animal studies and human clinical trials[J].Stem Cells Int,2015,2015:946031.
[5]Alini M,Eisenstein SM,Ito K,et al.Are animal models useful for studying human disc disorders/degeneration?[J].Eur Spine J,2008,17(1):2-19.
[6]Gullbrand SE,Malhotra NR,Schaer TP,et al.A large animal model that recapitulates the spectrum of human intervertebral disc degeneration[J].Osteoarthritis Cartilage,2017,25(1):146-156.
[7]Oehme D,Goldschlager T,Rosenfeld J,et al.Lateral surgical approach to lumbar intervertebral discs in an ovine model[J].Scientific World J,2012,2012:873726.
[8]Daly C,Ghosh P,Jenkin G,et al.A review of animal models of intervertebral disc degeneration:pathophysiology,regeneration,and translation to the clinic[J].Biomed Res Int,2016,2016:5952165.
[9]罗平,刘玉林,陈仲,等.纤维环穿刺法与纤维环切开法建立兔椎间盘退变模型[J].中国组织工程研究与临床康复,2010,14(11):1955-1958.
[10]康然,黄桂成,李海声,等.纤维环切开法建立猪腰椎间盘退变动物模型[J].实用老年医学,2012,2(2):141-145.
[11]Melrose J,Roberts S,Smith S,et al.Increased nerve and blood vessel ingrowth associated with proteoglycan depletion in an ovine anular lesion model of experimental disc degeneration[J].Spine(Phila Pa 1976),2002,27(12):1278-1285.
[12]彭俊,徐建广.犬椎间盘经皮针刺退变模型的建立[J].中国组织工程研究,2012,16(24):4463-4466.
[13]谢林,顾军,唐田,等.山羊腰椎间盘退变微创动物模型研究[J].实用骨科杂志,2015,(8):709-713.
[14]Lim KZ,Daly CD,Ghosh P,et al.Ovine lumbar intervertebral disc degeneration model utilizing a lateral retroperitoneal drill bit injury[J].J Vis Exp,2017,25(123).
[15]Zhang Y,Drapeau S,An HS,et al.Histological features of the degenerating intervertebral disc in a goat discinjury model[J].Spine(Phila Pa 1976),2011,36(19):1519-1527.
[16]张闻力,李涛,龚全,等.三种方法建立兔椎间盘退变模型的比较研究[J].中国修复重建外科杂志.2010,24(1):41-45.
[17]Le Maitre CL,Pockert A,Buttle DJ,et al.Matrix synthesis and degradation in human intervertebral disc degeneration[J].Biochem Soc Trans,2007,35(Pt 4):652-655.
[18]Acosta FL Jr,Metz L,Adkisson HD,et al.Porcine intervertebral disc repair using allogeneic juvenile articular chondrocytes or mesenchymal stem cells[J].Tissue Eng Part A,2011,17(23-24):3045-3055.
[19]Woiciechowsky C,Abbushi A,Zenclussen ML,et al.Regeneration of nucleus pulposus tissue in an ovine intervertebral disc degeneration model by cell-free resorbable polymer scaffolds[J].J Tissue Eng Regen Med,2014,8(10):811-820.
[20]黄永灿,吕维加,陆瓞骥.干细胞与椎间盘再生:从基础到临床[J].中国矫形外科杂志,2012,20(14):1299-1301.
[21]Hoogendoorn RJ,Wuisman PI,Smit TH,et al.Experimental intervertebral disc degeneration induced by chondroitinase ABC in the goat[J].Spine(Phila Pa1976),2007,32(17):1816-1825.
[22]Gullbrand SE,Malhotra NR,Schaer TP,et al.A large animal model that recapitulates the spectrum of human intervertebral disc degeneration[J].Osteoarthritis Cartilage,2017,25(1):146-156.
[23]Yamada K,Tanabe S,Ueno H,et al.Investigation of the short-term effect of chemonucleolysis with chondroitinase ABC[J].J Vet Med Sci,2001,63(5):521-525.
[24]Melrose J,Taylor TK,Ghosh P,et al.Intervertebral disc reconstitution after chemonucleolysis with chymopapain is dependent on dosage[J].Spine(Phila Pa 1976),1996,21(1):9-17.
[25]任大江,李放.建立椎间盘退变动物模型的方法学回顾[J].中国临床康复,2006,10(8):138-140.
[26]Cinotti G,Della Rocca C,Romeo S,et al.Degenerative changes of porcine intervertebral disc induced by vertebral endplate injuries[J].Spine(Phila Pa 1976),2005,30(2):174-180.
[27]Holm S,Holm AK,Ekstr?m L,et al.Experimental disc degeneration due to endplate injury[J].J Spinal Disord Tech,2004,17(1):64-71.
[28]Wei F,Zhong R,Zhou Z,et al.In vivo experimental intervertebral disc degeneration induced by bleomycin in the rhesus monkey[J].BMC Musculoskelet Disord,2014,15:340.
[29]Wei F,Zhong R,Wang L,et al.Pingyangmycininduced in vivo lumbar disc degeneration model of rhesus monkeys[J].Spine(Phila Pa 1976),2015,40(4):E199-210.
[30]Kang R,Li H,Ringgaard S,et al.Interference in the endplate nutritional pathway causes intervertebral disc degeneration in an immature porcine model[J].Int Orthop,2014,38(5):1011-1017.
[31]Bergknut N,Rutges JP,Kranenburg HJ,et al.The dog as an animal model for intervertebral disc degeneration?[J].Spine(Phila Pa 1976),2012,37(5):351-358.
[32]Platenberg RC,Hubbard GB,Ehler WJ,et al.Spontaneous disc degeneration in the baboon model:magnetic resonance imaging and histopathologic correlation[J].J Med Primatol,2001,30(5):268-272.
[33]Nuckley DJ,Kramer PA,Del Rosario A,et al.Intervertebral disc degeneration in a naturally occurring primate model:radiographic and biomechanical evidence[J].J Orthop Res,2008,26(9):1283-1288.
[34]Yuan W,Che W,Jiang YQ,et al.Establishment of intervertebral disc degeneration model induced by ischemic sub-endplate in rat tail[J].Spine J,2015,15(5):1050-1059.
[35]Liang QQ,Zhou Q,Zhang M,et al.Prolonged upright posture induces degenerative changes in intervertebral discs in rat lumbar spine[J].Spine(Phila Pa 1976),2008,33(19):2052-2058.
[36]Nasto LA,Ngo K,Leme AS,et al.Investigating the role of DNA damage in tobacco smoking-induced spine degeneration[J].Spine J,2014,14(3):416-423.
[37]Oda H,Matsuzaki H,Tokuhashi Y,et al.Degeneration of intervertebral discs due to smoking:experimental assessment in a rat-smoking model[J].J Orthop Sci,2004,9(2):135-141.
[2]Hoy D,March L,Brooks P,et al.The global burden of low back pain:estimates from the Global Burden of Disease 2010 study[J].Ann Rheum Dis,2014,73(6):968-974.
[3]Molinos M,Almeida CR,Caldeira J,et al.Inflammation in intervertebral disc degeneration and regeneration[J].J R Soc Interface,2015,12(108):20150429.
[4]Oehme D,Goldschlager T,Ghosh P,et al.Cell-based therapies used to treat lumbar degenerative disc disease:a systematic review of animal studies and human clinical trials[J].Stem Cells Int,2015,2015:946031.
[5]Alini M,Eisenstein SM,Ito K,et al.Are animal models useful for studying human disc disorders/degeneration?[J].Eur Spine J,2008,17(1):2-19.
[6]Gullbrand SE,Malhotra NR,Schaer TP,et al.A large animal model that recapitulates the spectrum of human intervertebral disc degeneration[J].Osteoarthritis Cartilage,2017,25(1):146-156.
[7]Oehme D,Goldschlager T,Rosenfeld J,et al.Lateral surgical approach to lumbar intervertebral discs in an ovine model[J].Scientific World J,2012,2012:873726.
[8]Daly C,Ghosh P,Jenkin G,et al.A review of animal models of intervertebral disc degeneration:pathophysiology,regeneration,and translation to the clinic[J].Biomed Res Int,2016,2016:5952165.
[9]罗平,刘玉林,陈仲,等.纤维环穿刺法与纤维环切开法建立兔椎间盘退变模型[J].中国组织工程研究与临床康复,2010,14(11):1955-1958.
[10]康然,黄桂成,李海声,等.纤维环切开法建立猪腰椎间盘退变动物模型[J].实用老年医学,2012,2(2):141-145.
[11]Melrose J,Roberts S,Smith S,et al.Increased nerve and blood vessel ingrowth associated with proteoglycan depletion in an ovine anular lesion model of experimental disc degeneration[J].Spine(Phila Pa 1976),2002,27(12):1278-1285.
[12]彭俊,徐建广.犬椎间盘经皮针刺退变模型的建立[J].中国组织工程研究,2012,16(24):4463-4466.
[13]谢林,顾军,唐田,等.山羊腰椎间盘退变微创动物模型研究[J].实用骨科杂志,2015,(8):709-713.
[14]Lim KZ,Daly CD,Ghosh P,et al.Ovine lumbar intervertebral disc degeneration model utilizing a lateral retroperitoneal drill bit injury[J].J Vis Exp,2017,25(123).
[15]Zhang Y,Drapeau S,An HS,et al.Histological features of the degenerating intervertebral disc in a goat discinjury model[J].Spine(Phila Pa 1976),2011,36(19):1519-1527.
[16]张闻力,李涛,龚全,等.三种方法建立兔椎间盘退变模型的比较研究[J].中国修复重建外科杂志.2010,24(1):41-45.
[17]Le Maitre CL,Pockert A,Buttle DJ,et al.Matrix synthesis and degradation in human intervertebral disc degeneration[J].Biochem Soc Trans,2007,35(Pt 4):652-655.
[18]Acosta FL Jr,Metz L,Adkisson HD,et al.Porcine intervertebral disc repair using allogeneic juvenile articular chondrocytes or mesenchymal stem cells[J].Tissue Eng Part A,2011,17(23-24):3045-3055.
[19]Woiciechowsky C,Abbushi A,Zenclussen ML,et al.Regeneration of nucleus pulposus tissue in an ovine intervertebral disc degeneration model by cell-free resorbable polymer scaffolds[J].J Tissue Eng Regen Med,2014,8(10):811-820.
[20]黄永灿,吕维加,陆瓞骥.干细胞与椎间盘再生:从基础到临床[J].中国矫形外科杂志,2012,20(14):1299-1301.
[21]Hoogendoorn RJ,Wuisman PI,Smit TH,et al.Experimental intervertebral disc degeneration induced by chondroitinase ABC in the goat[J].Spine(Phila Pa1976),2007,32(17):1816-1825.
[22]Gullbrand SE,Malhotra NR,Schaer TP,et al.A large animal model that recapitulates the spectrum of human intervertebral disc degeneration[J].Osteoarthritis Cartilage,2017,25(1):146-156.
[23]Yamada K,Tanabe S,Ueno H,et al.Investigation of the short-term effect of chemonucleolysis with chondroitinase ABC[J].J Vet Med Sci,2001,63(5):521-525.
[24]Melrose J,Taylor TK,Ghosh P,et al.Intervertebral disc reconstitution after chemonucleolysis with chymopapain is dependent on dosage[J].Spine(Phila Pa 1976),1996,21(1):9-17.
[25]任大江,李放.建立椎间盘退变动物模型的方法学回顾[J].中国临床康复,2006,10(8):138-140.
[26]Cinotti G,Della Rocca C,Romeo S,et al.Degenerative changes of porcine intervertebral disc induced by vertebral endplate injuries[J].Spine(Phila Pa 1976),2005,30(2):174-180.
[27]Holm S,Holm AK,Ekstr?m L,et al.Experimental disc degeneration due to endplate injury[J].J Spinal Disord Tech,2004,17(1):64-71.
[28]Wei F,Zhong R,Zhou Z,et al.In vivo experimental intervertebral disc degeneration induced by bleomycin in the rhesus monkey[J].BMC Musculoskelet Disord,2014,15:340.
[29]Wei F,Zhong R,Wang L,et al.Pingyangmycininduced in vivo lumbar disc degeneration model of rhesus monkeys[J].Spine(Phila Pa 1976),2015,40(4):E199-210.
[30]Kang R,Li H,Ringgaard S,et al.Interference in the endplate nutritional pathway causes intervertebral disc degeneration in an immature porcine model[J].Int Orthop,2014,38(5):1011-1017.
[31]Bergknut N,Rutges JP,Kranenburg HJ,et al.The dog as an animal model for intervertebral disc degeneration?[J].Spine(Phila Pa 1976),2012,37(5):351-358.
[32]Platenberg RC,Hubbard GB,Ehler WJ,et al.Spontaneous disc degeneration in the baboon model:magnetic resonance imaging and histopathologic correlation[J].J Med Primatol,2001,30(5):268-272.
[33]Nuckley DJ,Kramer PA,Del Rosario A,et al.Intervertebral disc degeneration in a naturally occurring primate model:radiographic and biomechanical evidence[J].J Orthop Res,2008,26(9):1283-1288.
[34]Yuan W,Che W,Jiang YQ,et al.Establishment of intervertebral disc degeneration model induced by ischemic sub-endplate in rat tail[J].Spine J,2015,15(5):1050-1059.
[35]Liang QQ,Zhou Q,Zhang M,et al.Prolonged upright posture induces degenerative changes in intervertebral discs in rat lumbar spine[J].Spine(Phila Pa 1976),2008,33(19):2052-2058.
[36]Nasto LA,Ngo K,Leme AS,et al.Investigating the role of DNA damage in tobacco smoking-induced spine degeneration[J].Spine J,2014,14(3):416-423.
[37]Oda H,Matsuzaki H,Tokuhashi Y,et al.Degeneration of intervertebral discs due to smoking:experimental assessment in a rat-smoking model[J].J Orthop Sci,2004,9(2):135-141.