枸杞多糖对膀胱癌细胞的增殖及凋亡作用研究
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摘要
目的研究枸杞多糖对膀胱癌细胞株T-24的增殖及凋亡作用。方法将膀胱癌细胞株T-24分为对照组和低、中、高剂量实验组。空白对照组加入等量的生理盐水,低、中、高剂量实验组分别加入100,200,400μg·m L~(-1)的枸杞多糖。用相差显微镜观察各组膀胱癌细胞株T-24的形态学改变,用流式细胞术检测细胞周期及细胞凋亡率,用细胞计数法(CCK-8)检测各组膀胱癌T-24细胞的增殖情况,用Hoechst33258荧光染色法检测移植瘤细胞凋亡情况。结果倒置相差显微镜下显示,细胞培养过程中不同剂量组、同时间增殖情况不同(高剂量组<中剂量组<低剂量组)。CCK-8结果显示,枸杞多糖抑制膀胱癌细胞株T-24的增殖;流式细胞术检测发现,枸杞多糖促进膀胱癌细胞株T-24凋亡,对浓度有依赖性。高剂量实验组光密度值在S期为38.32±1.24,G2/M期为9.69±1.90,凋亡率为(30.31±1.24)%,与对照组30.29±1.24,30.25±1.23,(21.03±1.16)%相比,差异均有统计学意义(P<0.05)。结论枸杞多糖对膀胱癌细胞株T-24有抑制其增殖、促进凋亡的作用。
Objective To study the effect of lycium barbarum polysaccharide on the proliferation and apoptosis of bladder cancer cell line T-24.Methods The bladder cancer cell line T-24 was divided into control group and low,middle and high dose experimental groups.Control group was given equal amount of normal saline,low,middle and high dose experimental groups were given 100,200,400μg·m L~(-1)lycium barbarum polysaccharide.The morphological changes of bladder cancer cell line T-24 were observed by phase-contrast microscopy.The cell cycle and apoptosis rate were detected by flow cytometry.The T-24cells were detected by cell count kit-8(CCK-8).Proliferation of cells was detected by Hoechst 33258 fluorescent staining.Results The inverted phase contrast microscope showed that the proliferation of different dosage groups at the same time during cell culture was different(high dose group
Objective To study the effect of lycium barbarum polysaccharide on the proliferation and apoptosis of bladder cancer cell line T-24.Methods The bladder cancer cell line T-24 was divided into control group and low,middle and high dose experimental groups.Control group was given equal amount of normal saline,low,middle and high dose experimental groups were given 100,200,400μg·m L~(-1)lycium barbarum polysaccharide.The morphological changes of bladder cancer cell line T-24 were observed by phase-contrast microscopy.The cell cycle and apoptosis rate were detected by flow cytometry.The T-24cells were detected by cell count kit-8(CCK-8).Proliferation of cells was detected by Hoechst 33258 fluorescent staining.Results The inverted phase contrast microscope showed that the proliferation of different dosage groups at the same time during cell culture was different(high dose group
引文
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[2]YU J,ZHU T Y,WANG Z R,et al.A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer[J].Clin Cancer Res,2007,13(24):7296-7304.
[3]丁明孝.细胞生物学实验指南[M].2版.北京:高等教育出版社,2013:187-189.
[4]许川山,ALBERT WANG NING LEUNG.荧光染色法观察MPPa光动力诱导鼻咽癌细胞凋亡[J].中国医学物理学杂志,2005,22(1):401-403.
[5]杜立颖,冯仁青.流式细胞术[M].2版.北京:北京大学出版社,2014:201-206.
[6]刘艳秋,张可华,王运良,等.CCK-8和MTS法检测人羊膜上皮细胞增殖的比较[J].中国康复理论与实践,2012,18(9):827-830.
[7]陈杰.病理诊断免疫组化手册[M].北京:中国协和医科大学出版,2014:177-179.
[8]袁征,单铁英,潘秀兰,等.枸杞多糖诱导T细胞增殖和对肝癌细胞的抑制作用[J].中国实医药,2010,5(4):21-22.