补阳还五汤、少腹逐瘀汤、丹参饮对寒凝血瘀模型大鼠血小板形态与黏附的影响
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摘要
目的从血小板黏附以及纤溶功能分析补阳还五汤、少腹逐瘀汤、丹参饮对寒凝血瘀的治疗的机制。方法选取80只大鼠,按体质量随机分成空白组和寒凝血瘀模型组,补阳还五汤、少腹逐瘀汤和丹参饮高低剂量组,10只/组。大鼠复制寒凝血瘀模型,连续造模15 d,每日灌胃给药,末次颈总动脉取血,测定血小板相关的各项指标。结果与空白组比较,模型组血小板体积分布宽度(PDW)显著升高(P <0.01),纤维连接蛋白(FN)含量显著升高(P <0.05)。与模型组比较,各给药组PDW显著降低(P <0.05),血小板平均体积(MPV)显著降低(P <0.05);丹参饮低剂量组血管性血友病因子(vWF)、FN含量显著降低(P <0.05),其余无显著变化。结论补阳还五汤、少腹逐瘀汤、丹参饮可能是通过降低PDW、MPV,由此降低血小板活性,从而起到了抗栓的作用,其中丹参饮降低血浆内v WF和FN水平,保护内皮细胞,可能是治疗寒凝血瘀证的机制之一。
Objective According to platelet adhesion and fibrinolysis function, the therapeutic mechanism of Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction on cold coagulation and blood stasis was analyzed. Methods 80 rats were randomly divided into blank group and cold coagulation and blood stasis model group according to their body weight, and the low and high dose groups of Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction group, each group contained 10 rats. The rat model of cold coagulation and blood stasis was established, and the model was established for 15 d continuously, intragastric administration was given every day, and the blood samples were taken from the common carotid artery. Results Compared with the blank group, the PDW of the model group was significantly increased(P < 0.01), the content of FN was significantly increased(P < 0.05). Compared with the model group, each group PDW significantly decreased(P < 0.05), MPV were significantly decreased(P < 0.05). Danshen decoction low dose group vWF, FN was significantly decreased(P < 0.05), while no significant change. Conclusion Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction may reduce PDW and MPV, which can reduce the activity of platelet, and thus play a role in anti thrombosis. Danshen decoction can decrease the levels of vWF and FN in the plasma and protect the endothelial cells, which may be one of the mechanisms of the treatment of cold coagulation and blood stasis syndrome.
Objective According to platelet adhesion and fibrinolysis function, the therapeutic mechanism of Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction on cold coagulation and blood stasis was analyzed. Methods 80 rats were randomly divided into blank group and cold coagulation and blood stasis model group according to their body weight, and the low and high dose groups of Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction group, each group contained 10 rats. The rat model of cold coagulation and blood stasis was established, and the model was established for 15 d continuously, intragastric administration was given every day, and the blood samples were taken from the common carotid artery. Results Compared with the blank group, the PDW of the model group was significantly increased(P < 0.01), the content of FN was significantly increased(P < 0.05). Compared with the model group, each group PDW significantly decreased(P < 0.05), MPV were significantly decreased(P < 0.05). Danshen decoction low dose group vWF, FN was significantly decreased(P < 0.05), while no significant change. Conclusion Buyang Huanwu decoction, Shaofu Zhuyu decoction and Danshen decoction may reduce PDW and MPV, which can reduce the activity of platelet, and thus play a role in anti thrombosis. Danshen decoction can decrease the levels of vWF and FN in the plasma and protect the endothelial cells, which may be one of the mechanisms of the treatment of cold coagulation and blood stasis syndrome.
引文
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[12]牛雯颖,张玉昆,肖洪彬,等. 3首活血化瘀方对寒凝血瘀模型大鼠红细胞膜蛋白组成研究[J].吉林中医药,2017, 37(1):58-62.
[13]牛雯颖,张禹,肖洪彬,等.寒凝血瘀模型大鼠红细胞膜生物学研究[J].中国实验方剂学杂志, 2013, 19(12):204.
[14]吴玉生,张富霞,李士林.血瘀证血细胞参数与血液流变学指标的相关性研究[J].安徽中医学院学报, 1996,15(6):51-52.
[15]陈晖.血液病患者血小板MPV、PCT、PDW的检测意义[J].实用全科医学, 2006, 4(4):479.
[16] OSTLUND E, HANSSON LO, BREMME K. Fibronectin is a marker for organ involvement and may refl ect the severity of preeclampsia[J]. Hypertens Pregnancy, 2001, 20(1):79-87.
[17]廖琪,马晓艳,真酌.FN、vWF、GPIb在子痫前期及子痫患者的检测及意义[J].中国伤残医学, 2009, 17(5):30.
[18]綦丽霞,王建荣. VWF检测指导低分子肝素治疗子痫前期的临床研究[J].河北医学, 2015, 21(11):1834-1837.
[19]戴霁菲,朱立新,蔡学林,等.血液病患者出血程度与PMP、vWF、FN的关系及临床意义[J].安徽医科大学学报, 2017, 52(7):1050-1053.
[20]李庆生,吴竞生.纤维连接蛋白在止血及血栓形成中的作用[J].血栓与止血学, 2005, 11(1):38-39.
[21] BEUMER S, HEIJNEN HF, IJSSELDIJK MJ, et al. Platelet adhesion to fiborneetin in flow:the importance of von Willebrand factor and glycoproteinⅠb[J].Blood, 1995,86(9):3452-3460.
[22] WUYP, VINK T, SCHIPHORST M, et al. Platelet thrombus formation on collagen at high shear rates is mediated by von Willebrand factor-glycoprotein Ib interaction and inhibited by von Willebrand factor-glycoproteinⅡb/Ⅲa interaction[J].Arterioscler Thromb Vasc Boil, 2000, 20(6):1661-1667.
[2]何淑虹.丹参对花生四烯酸及血脂调节的大鼠实验研究[J].浙江中西医结合杂志, 2005, 15(12):749-750.
[3]高莹,杨积武.栝蒌薤白半夏汤合丹参饮对痰浊血瘀型冠心病患者血脂及血小板功能的影响[J].辽宁中医杂志,2011, 38(2):307-308.
[4]华永庆,段金廒,宿树兰,等.用于不同证型痛经的四物汤类方生物效应评价[J].中国药科大学学报, 2008, 39(1):72.
[5]张帆,张春,李臻.小茴香及其炮制品挥发油对血瘀模型大鼠血液流变性的影响[J].中药药理与临床, 2010,26(5):81-82.
[6]秦华珍,李世阳,黄燕琼,等.干姜、高良姜、丁香3种提取物对寒证大鼠交感神经-肾上腺机能的影响[J].中国实验方剂学杂志, 2010, 16(14):124-127.
[7]安福丽,张仲,相聪坤,等.肉桂挥发油成分分析及其血小板聚集抑制作用研究[J].中国药业, 2009, 18(22):25-27.
[8]徐红梅,刘青云,戴敏,等.赤芍总苷对大鼠血液流变学的影响[J].中国中医药信息杂志, 2002, 9(11):17-19.
[9]王琳琳,丁安伟.赤芍总苷对大鼠血瘀证模型的影响[J].南京中医药大学学报, 2011, 27(6):552-554.
[10]王恩军,靳祎,王亮,等.蒲黄抑制大鼠血栓形成的实验研究[J].军医进修学院学报, 2008, 29(3):227-228.
[11]杨慧玲,李军.蒲黄总黄酮对家兔血液流变学参数和血小板聚集的影响[J].中国实验方剂学杂志, 2012,18(17):244-246.
[12]牛雯颖,张玉昆,肖洪彬,等. 3首活血化瘀方对寒凝血瘀模型大鼠红细胞膜蛋白组成研究[J].吉林中医药,2017, 37(1):58-62.
[13]牛雯颖,张禹,肖洪彬,等.寒凝血瘀模型大鼠红细胞膜生物学研究[J].中国实验方剂学杂志, 2013, 19(12):204.
[14]吴玉生,张富霞,李士林.血瘀证血细胞参数与血液流变学指标的相关性研究[J].安徽中医学院学报, 1996,15(6):51-52.
[15]陈晖.血液病患者血小板MPV、PCT、PDW的检测意义[J].实用全科医学, 2006, 4(4):479.
[16] OSTLUND E, HANSSON LO, BREMME K. Fibronectin is a marker for organ involvement and may refl ect the severity of preeclampsia[J]. Hypertens Pregnancy, 2001, 20(1):79-87.
[17]廖琪,马晓艳,真酌.FN、vWF、GPIb在子痫前期及子痫患者的检测及意义[J].中国伤残医学, 2009, 17(5):30.
[18]綦丽霞,王建荣. VWF检测指导低分子肝素治疗子痫前期的临床研究[J].河北医学, 2015, 21(11):1834-1837.
[19]戴霁菲,朱立新,蔡学林,等.血液病患者出血程度与PMP、vWF、FN的关系及临床意义[J].安徽医科大学学报, 2017, 52(7):1050-1053.
[20]李庆生,吴竞生.纤维连接蛋白在止血及血栓形成中的作用[J].血栓与止血学, 2005, 11(1):38-39.
[21] BEUMER S, HEIJNEN HF, IJSSELDIJK MJ, et al. Platelet adhesion to fiborneetin in flow:the importance of von Willebrand factor and glycoproteinⅠb[J].Blood, 1995,86(9):3452-3460.
[22] WUYP, VINK T, SCHIPHORST M, et al. Platelet thrombus formation on collagen at high shear rates is mediated by von Willebrand factor-glycoprotein Ib interaction and inhibited by von Willebrand factor-glycoproteinⅡb/Ⅲa interaction[J].Arterioscler Thromb Vasc Boil, 2000, 20(6):1661-1667.